Published ahead of print on March 1, 2007, doi:10.1164/rccm.200607-941OC Am. J. Respir. Crit. Care Med., Volume 175, Number 11, June 2007, 1158-1164 A more recent version of this article appeared on June 1, 2007
Submitted on July 12, 2006 Lung Cells From Neonates Show a Mesenchymal Stem Cell PhenotypeKenneth T Hennrick1,1 Departments of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, USA, 2 Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA, 3 Departments of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, USA; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA * To whom correspondence should be addressed. E-mail: mhershen{at}umich.edu.
Rationale: Mesenchymal stem cells have been isolated from adult bone marrow, peripheral blood, adipose tissue, trabecular bone, articular synovium and bronchial submucosa.
Objectives: We hypothesized that the lungs of premature infants undergoing mechanical ventilation contain fibroblast-like cells with features of mesenchymal stem cells. Methods: Tracheal aspirate fluid from mechanically-ventilated premature (< 30 wk gestation) infants Key words: bronchopulmonary dysplasia, fibroblast, monocyte chemoattractant protein, prematurity
This article has been cited by other articles:
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7 days of age was obtained from routine suctioning and plated on plastic culture dishes. Measurements and Main Results: Eleven of twenty patients studied demonstrated fibroblast-like cells, which were identified as early as 6 h after plating. Cells were found to express the mesenchymal stem cell markers STRO-1, CD73, CD90, CD105 and CD166, as well as CCR2b, CD13, prolyl 4-hydroxylase and 
-smooth muscle actin. Cells were negative for the hematopoietic and endothelial cell markers CD11b, CD31, CD34 or CD45. Tracheal aspirate monocyte chemoattractant protein-1 levels were 9-fold higher in aspirates in which fibroblastlike
cells were found, and cells demonstrated chemotaxis in response to monocyte chemoattractant protein. Placement of cells into appropriate media resulted in adipogenic,
osteogenic and myofibroblastic differentiation. Patients from whom mesenchymal stem cells were isolated tended to require more days of mechanical ventilation and supplemental oxygen. Conclusions: Together, these data demonstrate that tracheal aspirate fluid from premature,
mechanically-ventilated infants contains fibroblasts with cell markers and differentiation potential typically found in mesenchymal stem cells.
