Published ahead of print on March 30, 2006, doi:10.1164/rccm.200509-1461OC Am. J. Respir. Crit. Care Med., Volume 174, Number 1, July 2006, 31-40 A more recent version of this article appeared on July 1, 2006
Submitted on September 19, 2005 Impaired Alveolar Macrophage Response to Haemophilus Antigens in Chronic Obstructive Lung DiseaseCharles S Berenson1*,1 Department of Veterans Affairs Western New York Healthcare System, Infectious Disease Division, State University of New York at Buffalo School of Medicine, Buffalo, New York, USA, 2 Laboratory of Flow Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA * To whom correspondence should be addressed. E-mail: berenson{at}acsu.buffalo.edu.
Rationale: Interactions of nontypeable Haemophilus influenzae (NTHI) with macrophages are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). However, the immunologic mechanisms that mediate NTHI-macrophage inflammation are poorly understood. Outer membrane protein (OMP) P6 and lipooligosaccharide (LOS) of NTHI are potent immunomodulators. We theorized that alveolar macrophages in COPD possess fundamental immune defects that permit NTHI to evade host responses. Objective: To test this hypothesis, we obtained human alveolar and blood macrophages from: 1) ex-smokers with COPD, 2) ex-smokers without COPD and 3) nonsmokers. Methods: Alveolar and blood macrophages of each donor were incubated with purified LOS and OMP P6, as well as OMP P2 and the total outer membrane (OM) preparation (0.1-1ug/ml). Measurements: Supernatants (24 hours) were assayed for IL-1 Key words: nontypeable Haemophilus influenzae; macrophage; alveolar; cytokine; COPD
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