help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on May 18, 2006, doi:10.1164/rccm.200509-1420OC

Am. J. Respir. Crit. Care Med., Volume 174, Number 4, August 2006, 379-385

A more recent version of this article appeared on August 15, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200509-1420OCv1
174/4/379    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chan, V.
Right arrow Articles by Hirst, S. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chan, V.
Right arrow Articles by Hirst, S. J

Submitted on September 12, 2005
Accepted on May 16, 2006

Extracellular Matrix Regulates Enhanced Eotaxin Expression in Asthmatic Airway Smooth Muscle Cells

Vivien Chan1, Janette K Burgess2, Jonathan C Ratoff3, Brian J O'Connor3, Anne Greenough1, Tak H Lee1, and Stuart J Hirst1*

1 MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, King's College London School of Medicine, London, United Kingdom; Division of Asthma, Allergy and Lung Biology, King's College London School of Medicine, London, United Kingdom, 2 Woolcock Institute of Medical Research, Department of Pharmacology, University of Sydney, Sydney, Australia, 3 Division of Asthma, Allergy and Lung Biology, King's College London School of Medicine, London, United Kingdom

* To whom correspondence should be addressed. E-mail: stuart.hirst{at}kcl.ac.uk.

Rationale: Altered airway smooth muscle (ASM) function and enrichment of the extracellular matrix (ECM) with fibronectin and collagen are key features of asthma. Previously, we have reported these ECM proteins enhance ASM synthetic function. Objective: We compared ASM cultured from endobronchial biopsies from subjects with and without asthma to assess if asthmatic cells were hypersecretory and determined whether the underlying mechanism involved autocrine ECM production. Methods and measurements: Cells from subjects with and without asthma were cultured on plastic or in plates pre-coated with ECM proteins. Cytokine production was evaluated by enzyme-linked immunosorbent assay and by reverse transcriptase-polymerase chain reaction. Function blocking integrin antibodies were used to identify integrin involvement. Results: Baseline eotaxin and its production following stimulation with interleukin (IL)-13, IL-1{beta} or tumor necrosis factor-{alpha} was increased (2.5-to-6.0-fold) in ASM cells cultured from asthmatics compared with healthy subjects. When seeded on ECM from asthmatic ASM, IL-13-dependent eotaxin release from healthy or asthmatic ASM was enhanced compared with culture on healthy ECM. The ECM substrates fibronectin and type I collagen each enhanced IL-13-dependent eotaxin release and Western immunoblot indicated that fibronectin expression was higher in asthmatic ASM cells. Integrin blocking antibodies revealed that {alpha}5{beta}1 was required for more than 50% of the enhanced IL-13-dependent eotaxin release by ASM cells from asthmatics whilst {alpha}2{beta}1 or {alpha}v{beta}3 neutralization lacked effect. Conclusion: The data indicate that ASM cultured from asthmatics are hypersecretory compared with cells from healthy donors and that autocrine fibronectin secretion acting via {alpha}5{beta}1 in part underlies this effect.
Key words: airway remodeling, airway smooth muscle, eotaxin, extracellular matrix, integrin




This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. E. John, Y. M. Zhu, C. E. Brightling, L. Pang, and A. J. Knox
Human Airway Smooth Muscle Cells from Asthmatic Individuals Have CXCL8 Hypersecretion Due to Increased NF-{kappa}B p65, C/EBP{beta}, and RNA Polymerase II Binding to the CXCL8 Promoter
J. Immunol., October 1, 2009; 183(7): 4682 - 4692.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Mahn, S. J. Hirst, S. Ying, M. R. Holt, P. Lavender, O. O. Ojo, L. Siew, D. E. Simcock, C. G. McVicker, V. Kanabar, et al.
Diminished sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) expression contributes to airway remodelling in bronchial asthma
PNAS, June 30, 2009; 106(26): 10775 - 10780.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Fukuyama, T. Nakano, T. Matsumoto, B. G. G. Oliver, J. K. Burgess, A. Moriwaki, K. Tanaka, M. Kubo, T. Hoshino, H. Tanaka, et al.
Pulmonary Suppressor of Cytokine Signaling-1 Induced by IL-13 Regulates Allergic Asthma Phenotype
Am. J. Respir. Crit. Care Med., June 1, 2009; 179(11): 992 - 998.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. C. Ceresa, A. J. Knox, and S. R. Johnson
Use of a three-dimensional cell culture model to study airway smooth muscle-mast cell interactions in airway remodeling
Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L1059 - L1066.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Z. Li, S. Garantziotis, W. Jia, E. N. Potts, S. Lalani, Z. Liu, Y.-W. He, W. M. Foster, and J. W. Hollingsworth
The extracellular matrix protein mindin regulates trafficking of murine eosinophils into the airspace
J. Leukoc. Biol., January 1, 2009; 85(1): 124 - 131.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. E. Simcock, V. Kanabar, G. W. Clarke, K. Mahn, C. Karner, B. J. O'Connor, T. H. Lee, and S. J. Hirst
Induction of Angiogenesis by Airway Smooth Muscle From Patients with Asthma
Am. J. Respir. Crit. Care Med., September 1, 2008; 178(5): 460 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. Zuyderduyn, M. B. Sukkar, A. Fust, S. Dhaliwal, and J. K. Burgess
Treating asthma means treating airway smooth muscle cells
Eur. Respir. J., August 1, 2008; 32(2): 265 - 274.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
H. Meurs, R. Gosens, and J. Zaagsma
Airway hyperresponsiveness in asthma: lessons from in vitro model systems and animal models
Eur. Respir. J., August 1, 2008; 32(2): 487 - 502.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
J. E. Ward, T. Harris, T. Bamford, A. Mast, M. C. F. Pain, C. Robertson, D. Smallwood, T. Tran, J. Wilson, and A. G. Stewart
Proliferation is not increased in airway myofibroblasts isolated from asthmatics
Eur. Respir. J., August 1, 2008; 32(2): 362 - 371.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. Siddiqui, F. Hollins, and C. E. Brightling
What can we learn about airway smooth muscle from the company it keeps?
Eur. Respir. J., July 1, 2008; 32(1): 9 - 11.
[Full Text] [PDF]

Home page
 Eur Respir JHome page
B. B. Araujo, M. Dolhnikoff, L. F. F. Silva, J. Elliot, J. H. N. Lindeman, D. S. Ferreira, A. Mulder, H. A. P. Gomes, S. M. Fernezlian, A. James, et al.
Extracellular matrix components and regulators in the airway smooth muscle in asthma
Eur. Respir. J., July 1, 2008; 32(1): 61 - 69.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Farahi, A. S. Cowburn, P. D. Upton, J. Deighton, A. Sobolewski, E. Gherardi, N. W. Morrell, and E. R. Chilvers
Eotaxin-1/CC Chemokine Ligand 11: A Novel Eosinophil Survival Factor Secreted by Human Pulmonary Artery Endothelial Cells
J. Immunol., July 15, 2007; 179(2): 1264 - 1273.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. C. Moore and S. P. Peters
Update in Asthma 2006
Am. J. Respir. Crit. Care Med., April 1, 2007; 175(7): 649 - 654.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. J. Ammit, L. M. Moir, B. G. Oliver, J. M. Hughes, H. Alkhouri, Q. Ge, J. K. Burgess, J. L. Black, and M. Roth
Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L199 - L206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. E. Wenzel and S. Balzar
Myofibroblast or smooth muscle: do in vitro systems adequately replicate tissue smooth muscle?
Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 364 - 365.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2006 American Thoracic Society 		  ATS 2008 State of the Art Course