Published ahead of print on June 9, 2005, doi:10.1164/rccm.200503-439OC Am. J. Respir. Crit. Care Med., Volume 172, Number 6, September 2005, 738-744 A more recent version of this article appeared on September 15, 2005
Submitted on March 21, 2005 3P Microsatellite Alterations in Exhaled Breath Condensate from Non-small Cell Lung Cancer PatientsGiovanna Elisiana Carpagnano1*,1 Institute of Respiratory Disease, University of Foggia, Foggia, Italy, 2 ISPA, CNR, Bari, Italy, 3 Institute of Respiratory Disease, University of Bari, Bari, Italy, 4 Clinical Experimental Oncology Lab, National Cancer Institute, Bari, Italy, 5 Department of Thoracic Surgery, San Paolo Hospital, Bari, Italy * To whom correspondence should be addressed. E-mail: ge.carpagnano{at}unifg.it.
Rationale: The still high mortality for lung cancer urgently requires the availability of new noninvasive diagnostic tools destined to more effective early diagnosis and screening programs. Recently, exhaled breath condensate (EBC) has been proposed as a useful tool to obtain biological information on lung cancer disease. Objectives: The present study checked for the feasibility of microsatellite alteration (MA) analysis in the EBC-DNA from NSCLC patients with respect to alterations found in whole blood (WB) DNA. Methods: Thirty patients with a histological evidence of NSCLC and 20 healthy subjects were enrolled. All subjects had allelotyping analysis on DNA from EBC and WB-DNA of a panel of microsatellites (D3S2338, D3S1266, D3S1300, D3S1304, D3S1289) located in chromosomal region 3p. Results from healthy and cancer subjects, and from EBC and WB were compared. Furthermore, the relationships with smoking habit and clinical- pathological tumour features were considered. Measurements and Main Results: MA were found in 53% of EBC-DNA and in 10% of WB-DNA loci investigated from NSCLC patients (p< 10-6); conversely, MA were present only in 13% in EBC-DNA and 2% in WB-DNAs informative loci of healthy subjects. In NSCLC patients a direct association between number of MA detected in EBC-DNA and tobacco consumption was observed. Conclusions: In the present paper, for the first time we provide evidence that EBC-DNA is highly sensitive in detecting MA from NSCLC and healthy subjects. Furthermore, MA information seem to be directly related with tobacco consumption and then, potentially applicable to screening and early diagnosis programs for NSCLC patients. Key words: DNA,LOH,chromosome 3, NSCLC susceptibility
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