help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on September 22, 2005, doi:10.1164/rccm.200412-1714OC

Am. J. Respir. Crit. Care Med., Volume 172, Number 11, December 2005, 1412-1415

A more recent version of this article appeared on December 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200412-1714OCv1
172/11/1412    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Plant, B. J
Right arrow Articles by Donnelly, S. C
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Plant, B. J
Right arrow Articles by Donnelly, S. C

Submitted on December 21, 2004
Accepted on September 20, 2005

Cystic Fibrosis, Disease Severity and a Macrophage Migration Inhibitory Factor Polymorphism

Barry J Plant1, Charles G Gallagher1, Richard Bucala2, John A Baugh1, Sally Chappell3, Linda Morgan3, Clare M O'Connor1, Kevin Morgan3, and Seamas C Donnelly1*

1 Department of Medicine, University College Dublin, The Conway Institute of Biomolecular and Biomedical Research and The Dublin Molecular Medicine Centre, Dublin, Ireland, 2 Department of Medicine and Pathology, Yale University School of Medicine, New Haven, Connecticut, USA, 3 Queen's Medical Centre, University of Nottingham, Institute of Genetics, School of Molecular Medical Sciences, Nottingham, England, United Kingdom

* To whom correspondence should be addressed. E-mail: seamas.donnelly{at}ucd.ie.

RATIONALE: MIF is a key pro-inflammatory mediator. It contributes towards an exaggerated gramnegative inflammatory response via its ability to induce TLR4 (Toll like receptor-4) expression. Studies have shown that MIF knock-out mice have less aggressive pseudomonas infection (compared to wild-type.) OBJECTIVES: To assess whether a novel functional MIF polymorphism was associated with clinical prognosis in a patient cohort with chronic gram-negative infection, namely Cystic Fibrosis. METHODS: Collected Genomic DNA was analysed via PCR amplification for the polymorphic region for the CATT repeat polymorphism. Individuals may have a 5,6,7 or 8-CATT tetranucleotide repeat unit on each allele. The 5-CATT repeat allele exhibits the lowest MIF promoter activity. MEASUREMENTS AND MAIN RESULTS: Stable CF patients (n=167) and a matched control group (n=166) were enrolled. In patients with CF, the MIF5+ group had a decreased incidence of Pseudomonas aeruginosa colonisation (Odds ratio 0.25; 95% confidence interval 0.09 to 0.65; p=0.004) and a significant reduction in the risk of pancreatic insufficiency (Odds ratio 0.27; 95% confidence interval 0.07 to 1.0; p =0.05). A trend towards milder disease activity in the MIF5+ group was seen with all other parameters. CONCLUSIONS: The results support the concept of a regulatory role for MIF in CF.
Key words: MIF, polymorphism, cystic fibrosis.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. A. Kevill, V. Bhandari, M. Kettunen, L. Leng, J. Fan, Y. Mizue, J. D. Dzuira, M. Reyes-Mugica, C. L. McDonald, J. A. Baugh, et al.
A Role for Macrophage Migration Inhibitory Factor in the Neonatal Respiratory Distress Syndrome
J. Immunol., January 1, 2008; 180(1): 601 - 608.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
H. Flaster, J. Bernhagen, T. Calandra, and R. Bucala
The Macrophage Migration Inhibitory Factor-Glucocorticoid Dyad: Regulation of Inflammation and Immunity
Mol. Endocrinol., June 1, 2007; 21(6): 1267 - 1280.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
B. J. Plant, S. Ghani, M. J. O'Mahony, L. Morgan, C. M. O'Connor, K. Morgan, J. A. Baugh, and S. C. Donnelly
Sarcoidosis and MIF gene polymorphism: a case-control study in an Irish population
Eur. Respir. J., February 1, 2007; 29(2): 325 - 329.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. F. McKone, J. Shao, D. D. Frangolias, C. L. Keener, C. A. Shephard, F. M. Farin, M. R. Tonelli, P. D. Pare, A. J. Sandford, M. L. Aitken, et al.
Variants in the Glutamate-Cysteine-Ligase Gene Are Associated with Cystic Fibrosis Lung Disease
Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 415 - 419.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
F. J. Accurso
Update in cystic fibrosis 2005.
Am. J. Respir. Crit. Care Med., May 1, 2006; 173(9): 944 - 947.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2005 American Thoracic Society 		  ATS Clinical Skills Tests