help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on October 22, 2004, doi:10.1164/rccm.200408-1046OC

Am. J. Respir. Crit. Care Med., Volume 171, Number 3, February 2005, 217-223

A more recent version of this article appeared on February 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200408-1046OCv1
171/3/217    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nguyen, T. T-B
Right arrow Articles by Hirst, S. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nguyen, T. T-B
Right arrow Articles by Hirst, S. J

Submitted on August 17, 2004
Accepted on October 18, 2004

{beta}1 Integrins Mediate Enhancement of Airway Smooth Muscle Proliferation by Collagen and Fibronectin

Trang T-B Nguyen1, Jeremy P.T. Ward1, and Stuart J Hirst1*

1 Department of Asthma, Allergy and Respiratory Science, The Guy's, King's and St. Thomas' School of Medicine, King's College London, London, United Kingdom

* To whom correspondence should be addressed. E-mail: stuart.hirst{at}kcl.ac.uk.

Airway smooth muscle (ASM) accumulation and enrichment of the extracellular matrix (ECM) with type I collagen and fibronectin are major pathological features of airway remodeling in asthma. These ECM components confer enhanced ASM proliferation in vitro but a requirement for specific integrin ECM receptors has not been examined. Here, we examined the mitogen, platelet-derived growth factor (PDGF)-BB on {beta}1-integrin expression on human ASM cells cultured on these ECM substrates and defined the involvement of specific integrins in cell attachment and proliferation using integrin-neutralizing antibodies. PDGF-BB-dependent proliferation was enhanced 2- to 3-fold by monomeric type I collagen or fibronectin and to a lesser extent by vitronectin; other interstitial ECM components (fibrillar type I and III collagen and tenascin-C) had no effect. Excepting increased {alpha}3 expression induced by PDGF-BB and monomeric type I collagen or fibronectin, {alpha}1, {alpha}2, {alpha}4, {alpha}5, {alpha}v and {alpha}v{beta}3 integrins were unchanged compared with unstimulated cells on plastic. Blocking antibodies revealed {alpha}2{beta}1 and {alpha}v{beta}3 mediated attachment to monomeric type I collagen, whereas attachment to fibronectin required {alpha}5{beta}1. In contrast, enhancement of PDGF-BB-dependent proliferation by either monomeric type I collagen or fibronectin required {alpha}2{beta}1, {alpha}4{beta}1 and {alpha}5{beta}1 integrins. These data suggest multiple {beta}1-integrins regulate enhanced ASM proliferative responses.
Key words: Asthma, airway remodeling, airway smooth muscle, proliferation, extracellular matrix




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. G. J. Dekkers, D. Schaafsma, T. Tran, J. Zaagsma, and H. Meurs
Insulin-Induced Laminin Expression Promotes a Hypercontractile Airway Smooth Muscle Phenotype
Am. J. Respir. Cell Mol. Biol., October 1, 2009; 41(4): 494 - 504.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. Wu, H. Du, X. Wang, C. Mei, G. C. Sieck, and Q. Qian
Characterization of Primary Cilia in Human Airway Smooth Muscle Cells
Chest, August 1, 2009; 136(2): 561 - 570.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. M. Misior, D. A. Deshpande, M. J. Loza, R. M. Pascual, J. D. Hipp, and R. B. Penn
Glucocorticoid- and Protein Kinase A-Dependent Transcriptome Regulation in Airway Smooth Muscle
Am. J. Respir. Cell Mol. Biol., July 1, 2009; 41(1): 24 - 39.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
S. R. Singh and I. P. Hall
Airway Myofibroblasts and Their Relationship with Airway Myocytes and Fibroblasts
Proceedings of the ATS, January 1, 2008; 5(1): 127 - 132.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. Tran, K. Ens-Blackie, E. S. Rector, G. L. Stelmack, K. D. McNeill, G. Tarone, W. T. Gerthoffer, H. Unruh, and A. J. Halayko
Laminin-Binding Integrin {alpha}7 Is Required for Contractile Phenotype Expression by Human Airway Myocytes
Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 668 - 680.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. Lu, S. Xie, M. B. Sukkar, X. Lu, M. F. Scully, and K. F. Chung
Inhibition of Airway Smooth Muscle Adhesion and Migration by the Disintegrin Domain of ADAM-15
Am. J. Respir. Cell Mol. Biol., October 1, 2007; 37(4): 494 - 500.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. G. McVicker, S.-Y. Leung, V. Kanabar, L. M. Moir, K. Mahn, K. F. Chung, and S. J. Hirst
Repeated Allergen Inhalation Induces Cytoskeletal Remodeling in Smooth Muscle from Rat Bronchioles
Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 721 - 727.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. G. J. Dekkers, D. Schaafsma, S. A. Nelemans, J. Zaagsma, and H. Meurs
Extracellular matrix proteins differentially regulate airway smooth muscle phenotype and function
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1405 - L1413.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
E. D. Fixman, A. Stewart, and J. G. Martin
Basic mechanisms of development of airway structural changes in asthma
Eur. Respir. J., February 1, 2007; 29(2): 379 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. J. Ammit, L. M. Moir, B. G. Oliver, J. M. Hughes, H. Alkhouri, Q. Ge, J. K. Burgess, J. L. Black, and M. Roth
Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L199 - L206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
V. Chan, J. K. Burgess, J. C. Ratoff, B. J. O'Connor, A. Greenough, T. H. Lee, and S. J. Hirst
Extracellular Matrix Regulates Enhanced Eotaxin Expression in Asthmatic Airway Smooth Muscle Cells
Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 379 - 385.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2004 American Thoracic Society 		  CCM abstracts