help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on December 23, 2004, doi:10.1164/rccm.200407-953OC

Am. J. Respir. Crit. Care Med., Volume 171, Number 8, April 2005, 806-813

A more recent version of this article appeared on April 15, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200407-953OCv1
171/8/806    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hollingsworth II, J. W
Right arrow Articles by Schwartz, D. A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hollingsworth II, J. W
Right arrow Articles by Schwartz, D. A

Submitted on July 26, 2004
Accepted on December 13, 2004

The Critical Role of Hematopoietic Cells in Lipopolysaccharide Induced Airway Inflammation

John W Hollingsworth II1*, Benny J Chen2, David M Brass3, Katie Berman3, M. Dee Gunn4, Donald N Cook3, and David A Schwartz1

1 Division of Pulmonary, Allergy, and Critical Care Medicine, Duke University Medical Center, Durham, NC, USA; Pulmonary and Critical Care Medicine, Veterans Administration Medical Center, Durham, NC, USA, 2 Division of Cellular Therapy, Duke University Medical Center, Durham, NC, USA, 3 Division of Pulmonary, Allergy, and Critical Care Medicine, Duke University Medical Center, Durham, NC, USA, 4 Division of Cardiology, Duke University Medical Center, Durham, NC, USA

* To whom correspondence should be addressed. E-mail: holli017{at}mc.duke.edu.

Rapid and selective recruitment of neutrophils into the airspace in response to lipopolysaccharide (LPS) facilitates the clearance of bacterial pathogens. However, neutrophil infiltration can also participate in the development and progression of environmental airway disease. Previous data have revealed that toll-like receptor 4 (tlr4) is required for neutrophil recruitment to the lung following either inhaled or systemically administrated LPS from E. coli. While many cell types express tlr4, endothelial cell expression of tlr4 is specifically required to sequester neutrophils in the lung in response to systemic endotoxin. To identify the cell types requiring trl4 expression for neutrophil recruitment following inhaled LPS, we generated chimeric mice separately expressing tlr4 on either hematopoietic cells, or on structural lung cells. Neutrophil recruitment into the airspace was completely restored in tlr4-/- mice receiving wild type bone marrow. By contrast, wild type animals receiving tlr4-/- marrow had dramatically reduced neutrophil recruitment. Moreover, adoptive transfer of wild type alveolar macrophages also restored the ability of tlr4-/- recipient mice to recruit neutrophils to the lung. These data demonstrate the critical role of hematopoietic cells and alveolar macrophages in initiating LPS induced neutrophil recruitment from the vascular space to the airspace.
Key words: toll-like receptor, macrophage, neutrophil, innate immunity, lipopolysaccharide




This article has been cited by other articles:


Home page
 J. Immunol.Home page
L. C. Parker, E. C. Prestwich, J. R. Ward, E. Smythe, A. Berry, M. Triantafilou, K. Triantafilou, and I. Sabroe
A Phosphatidylserine Species Inhibits a Range of TLR- but Not IL-1{beta}-Induced Inflammatory Responses by Disruption of Membrane Microdomains
J. Immunol., October 15, 2008; 181(8): 5606 - 5617.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Zhao, J. W. Zmijewski, E. Lorne, G. Liu, Y.-J. Park, Y. Tsuruta, and E. Abraham
Activation of AMPK attenuates neutrophil proinflammatory activity and decreases the severity of acute lung injury
Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L497 - L504.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Hadina, J. P. Weiss, P. B. McCray Jr., K. Kulhankova, and P. S. Thorne
MD-2-Dependent Pulmonary Immune Responses to Inhaled Lipooligosaccharides: Effect of Acylation State
Am. J. Respir. Cell Mol. Biol., June 1, 2008; 38(6): 647 - 654.
[Abstract] [Full Text] [PDF]

Home page
 Postgrad. Med. J.Home page
I Sabroe, L C Parker, P M A Calverley, S K Dower, and M K B Whyte
Pathological networking: a new approach to understanding COPD
Postgrad. Med. J., May 1, 2008; 84(991): 259 - 264.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
K. P. Mollen, R. M. Levy, J. M. Prince, R. A. Hoffman, M. J. Scott, D. J. Kaczorowski, R. Vallabhaneni, Y. Vodovotz, and T. R. Billiar
Systemic inflammation and end organ damage following trauma involves functional TLR4 signaling in both bone marrow-derived cells and parenchymal cells
J. Leukoc. Biol., January 1, 2008; 83(1): 80 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
I. Sabroe, L. C. Parker, S. K. Dower, and M. K. B. Whyte
Practical and Conceptual Models of Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, December 1, 2007; 4(8): 606 - 610.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
I. Sabroe, L. C Parker, P. M A Calverley, S. K Dower, and M. K B Whyte
Pathological networking: a new approach to understanding COPD
Thorax, August 1, 2007; 62(8): 733 - 738.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. W. Hollingsworth, Z. Li, D. M. Brass, S. Garantziotis, S. H. Timberlake, A. Kim, I. Hossain, R. C. Savani, and D. A. Schwartz
CD44 Regulates Macrophage Recruitment to the Lung in Lipopolysaccharide-Induced Airway Disease
Am. J. Respir. Cell Mol. Biol., August 1, 2007; 37(2): 248 - 253.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. W. Hollingsworth, S. R. Kleeberger, and W. M. Foster
Ozone and Pulmonary Innate Immunity
Proceedings of the ATS, July 1, 2007; 4(3): 240 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Brass, J. W. Hollingsworth, E. McElvania-Tekippe, S. Garantziotis, I. Hossain, and D. A. Schwartz
CD14 is an essential mediator of LPS-induced airway disease
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L77 - L83.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
N. Chaudhuri, M. K. B. Whyte, and I. Sabroe
Reducing the Toll of Inflammatory Lung Disease
Chest, May 1, 2007; 131(5): 1550 - 1556.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
I. Sabroe, L. C. Parker, D. H. Dockrell, D. E. Davies, S. K. Dower, and M. K. B. Whyte
Targeting the Networks that Underpin Contiguous Immunity in Asthma and Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., February 15, 2007; 175(4): 306 - 311.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
G. E. Morris, L. C. Parker, J. R. Ward, E. C. Jones, M. K. B. Whyte, C. E. Brightling, P. Bradding, S. K. Dower, and I. Sabroe
Cooperative molecular and cellular networks regulate Toll-like receptor-dependent inflammatory responses
FASEB J, October 1, 2006; 20(12): 2153 - 2155.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Abraham, J. A. Nick, T. Azam, S. H. Kim, J.-P. Mira, D. Svetkauskaite, Q. He, M. Zamora, J. Murphy, J. S. Park, et al.
Peripheral blood neutrophil activation patterns are associated with pulmonary inflammatory responses to lipopolysaccharide in humans.
J. Immunol., June 15, 2006; 176(12): 7753 - 7760.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
A. E. Medvedev, I. Sabroe, J. D. Hasday, and S. N. Vogel
Invited review: Tolerance to microbial TLR ligands: molecular mechanisms and relevance to disease
Innate Immunity, June 1, 2006; 12(3): 133 - 150.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
J. W. Hollingsworth, G. S. Whitehead, K. L. Lin, H. Nakano, M. D. Gunn, D. A. Schwartz, and D. N. Cook
TLR4 Signaling Attenuates Ongoing Allergic Inflammation
J. Immunol., May 15, 2006; 176(10): 5856 - 5862.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. E. Wenzel and R. Covar
Update in asthma 2005.
Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 698 - 706.
[Full Text] [PDF]

Home page
 BloodHome page
J. W. Chien, L. P. Zhao, J. A. Hansen, W. H. Fan, T. Parimon, and J. G. Clark
Genetic variation in bactericidal/permeability-increasing protein influences the risk of developing rapid airflow decline after hematopoietic cell transplantation
Blood, March 1, 2006; 107(5): 2200 - 2207.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. M. Hajjar, H. Harowicz, H. D. Liggitt, P. J. Fink, C. B. Wilson, and S. J. Skerrett
An Essential Role for Non-Bone Marrow-Derived Cells in Control of Pseudomonas aeruginosa Pneumonia
Am. J. Respir. Cell Mol. Biol., November 1, 2005; 33(5): 470 - 475.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2004 American Thoracic Society 		  ATS State of the Art 2009