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Published ahead of print on June 7, 2004, doi:10.1164/rccm.200401-127OC

Am. J. Respir. Crit. Care Med., Volume 170, Number 5, September 2004, 516-519

A more recent version of this article appeared on September 1, 2004
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Submitted on January 29, 2004
Accepted on June 1, 2004

Discrimination of Human Lung Neoplasm from Normal Lung by Two Target Genes

Hans-Stefan Hofmann1*, Gesine Hansen2, Stefan Burdach3, Babett Bartling1, Rolf-Edgar Silber1, and Andreas Simm1

1 Department of Cardio-Thoracic Surgery, Martin-Luther University Halle-Wittenberg, Halle, Germany, 2 Children's Cancer Research Center and Department of Paediatrics, Martin-Luther University Halle-Wittenberg, Halle, Germany, 3 Department of Paediatrics, Technical University of Munich, Munich, Germany

* To whom correspondence should be addressed. E-mail: stefan.hofmann{at}medizin.uni-halle.de.

Simple tools for discrimination of lung tissues can be useful in a fast machine-aided diagnosis e.g. by tumour specific microarrays. We demonstrate that an easy ratio technique, based on the expression levels of only two genes differentially expressed in lung tumour and normal lung samples, allows discrimination of normal and neoplastic lung with a sensitivity of 100% and specificity of 90.5%. DNA microarray analysis of 99 lung tumour samples and 15 normal lung tissues revealed that receptor for advanced glycation end products (RAGE) mRNA is reduced four fold (p=7.8x10-11) and Cyclin-B2 mRNA is up-regulated two fold (p=5.9x10-18) in lung carcinoma compared to normal lung. The microarray-calculated expression ratio of RAGE/Cyclin-B2 was used in PCR analysis of 84 independent blinded samples to discriminate tumour and corresponding normal lung tissues. In 94.7 percent of the samples this quotient correctly distinguished non-small cell lung cancer from normal lung tissue, suggesting the RAGE/Cyclin-B2 quotient as a potential means for diagnosis of lung cancer.
Key words: Lung cancer, Lung metastases, Tissue-Discrimination, RAGE, Cyclin-B2




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