help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on October 22, 2004, doi:10.1164/rccm.200310-1416OC

Am. J. Respir. Crit. Care Med., Volume 171, Number 3, February 2005, 224-230

A more recent version of this article appeared on February 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200310-1416OCv1
171/3/224    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cho, S.-H.
Right arrow Articles by Johnston, S. L
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cho, S.-H.
Right arrow Articles by Johnston, S. L

Submitted on October 20, 2003
Accepted on October 18, 2004

Increased Interleukin-4,-5 and Interferon-{gamma} in Airway CD4+ and CD8+ T cells in Atopic Asthma

Sang-Heon Cho1, Luminita A Stanciu2*, Stephen T Holgate3, and Sebastian L Johnston2

1 Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea, Republic of, 2 Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom, 3 Infection, Inflammation and Repair Division, University of Southampton, Southampton, United Kingdom

* To whom correspondence should be addressed. E-mail: l.stanciu{at}imperial.ac.uk.

Increased Th2 cytokine production in asthma is widely accepted, but excess production by asthmatic human airway CD4+ T cells has not been demonstrated, nor has a relationship with disease severity. The importance of airway CD8+ T cell type 1 & type 2 cytokine production in asthma is unknown. We investigated frequencies of IFN-{gamma}, IL-4 and IL-5 producing CD4+ and CD8+ blood and sputum T cells from asthmatic and normal subjects and compared between cell subsets, subject groups and body compartments with and without in vitro stimulation and investigated relationships between cytokine production and asthma severity. Production of IL-4, IL-5 and IFN-{gamma} by unstimulated sputum CD4+ and CD8+ T cells was increased in asthmatics and related to disease severity, more for CD8+ than CD4+ T cells. Frequencies of sputum CD8+ T cells producing type 1 and type 2 cytokines were similar to those of CD4+ T cells. In vitro stimulation polarised peripheral blood cytokine production towards IFN-{gamma} production, significantly more in asthmatic than normal subjects. These data demonstrate increased type 1 and 2 cytokine production in CD4+ and CD8+ T cells in sputum and relate production to disease severity. Findings in blood did not reflect those in airways.
Key words: human; T lymphocytes; asthma; sputum; flow cytometry




This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y.-S. Kim, S.-W. Hong, J.-P. Choi, T.-S. Shin, H.-G. Moon, E.-J. Choi, S. G. Jeon, S.-Y. Oh, Y. S. Gho, Z. Zhu, et al.
Vascular Endothelial Growth Factor Is a Key Mediator in the Development of T Cell Priming and Its Polarization to Type 1 and Type 17 T Helper Cells in the Airways
J. Immunol., October 15, 2009; 183(8): 5113 - 5120.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
H.-B. Kim, C.-K. Kim, K. Iijima, T. Kobayashi, and H. Kita
Protein Microarray Analysis in Patients With Asthma: Elevation of the Chemokine PARC/CCL18 in Sputum
Chest, February 1, 2009; 135(2): 295 - 302.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Bosco and P. G. Holt
Genome-wide expression profiling of T-cells in childhood wheeze
Eur. Respir. J., November 1, 2008; 32(5): 1138 - 1140.
[Full Text] [PDF]

Home page
 JEMHome page
M. Okamoto, K. Takeda, A. Joetham, H. Ohnishi, H. Matsuda, C. H. Swasey, B. J. Swanson, K. Yasutomo, A. Dakhama, and E. W. Gelfand
Essential role of Notch signaling in effector memory CD8+ T cell-mediated airway hyperresponsiveness and inflammation
J. Exp. Med., May 12, 2008; 205(5): 1087 - 1097.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. Shannon, P. Ernst, Y. Yamauchi, R. Olivenstein, C. Lemiere, S. Foley, L. Cicora, M. Ludwig, Q. Hamid, and J. G. Martin
Differences in Airway Cytokine Profile in Severe Asthma Compared to Moderate Asthma*
Chest, February 1, 2008; 133(2): 420 - 426.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Joubert, S. Lajoie-Kadoch, M. Welman, S. Dragon, S. Letuvee, B. Tolloczko, A. J. Halayko, A. S. Gounni, K. Maghni, and Q. Hamid
Expression and Regulation of CCR1 by Airway Smooth Muscle Cells in Asthma
J. Immunol., January 15, 2008; 180(2): 1268 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Koya, N. Miyahara, K. Takeda, S. Matsubara, H. Matsuda, C. Swasey, A. Balhorn, A. Dakhama, and E. W. Gelfand
CD8+ T Cell-Mediated Airway Hyperresponsiveness and Inflammation Is Dependent on CD4+IL-4+ T Cells
J. Immunol., September 1, 2007; 179(5): 2787 - 2796.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. B. Early, E. Barekzi, J. Negri, K. Hise, L. Borish, and J. W. Steinke
Concordant Modulation of Cysteinyl Leukotriene Receptor Expression by IL-4 and IFN-{gamma} on Peripheral Immune Cells
Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 715 - 720.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y.-K. Kim, S.-Y. Oh, S. G. Jeon, H.-W. Park, S.-Y. Lee, E.-Y. Chun, B. Bang, H.-S. Lee, M.-H. Oh, Y.-S. Kim, et al.
Airway Exposure Levels of Lipopolysaccharide Determine Type 1 versus Type 2 Experimental Asthma
J. Immunol., April 15, 2007; 178(8): 5375 - 5382.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. E. Wenzel and R. Covar
Update in asthma 2005.
Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 698 - 706.
[Full Text] [PDF]

Home page
 ThoraxHome page
E Truyen, L Coteur, E Dilissen, L Overbergh, L J Dupont, J L Ceuppens, and D M A Bullens
Evaluation of airway inflammation by quantitative Th1/Th2 cytokine mRNA measurement in sputum of asthma patients
Thorax, March 1, 2006; 61(3): 202 - 208.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. N. Georas, J. Guo, U. De Fanis, and V. Casolaro
T-helper cell type-2 regulation in allergic disease
Eur. Respir. J., December 1, 2005; 26(6): 1119 - 1137.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. L. J. van Rensen, J. K. Sont, C. E. Evertse, L. N. A. Willems, T. Mauad, P. S. Hiemstra, P. J. Sterk, and the AMPUL Study Group
Bronchial CD8 Cell Infiltrate and Lung Function Decline in Asthma
Am. J. Respir. Crit. Care Med., October 1, 2005; 172(7): 837 - 841.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2004 American Thoracic Society 		  ATS Quiz on Sleep Study Tracings