help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on November 20, 2003, doi:10.1164/rccm.200307-1004OC

Am. J. Respir. Crit. Care Med., Volume 169, Number 5, March 2004, 573-577

A more recent version of this article appeared on March 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200307-1004OCv1
169/5/573    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dorman, S. C
Right arrow Articles by Sehmi, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dorman, S. C
Right arrow Articles by Sehmi, R.

Submitted on July 23, 2003
Accepted on November 19, 2003

Sputum CD34+IL-5R{alpha}+ cells increase after allergen: evidence for in situ eosinophilopoeisis

Sandra C Dorman1, Ann Efthimiadis2, Irene Babirad2, Rick M Watson1, Judah A Denburg1, Frederick E Hargreave2, Paul M O'Byrne1, and Roma Sehmi2*

1 Medicine, McMaster University, Hamilton, Ontario, Canada, 2 St. Joseph's Hospital, Firestone Institute for Respiratory Health, Hamilton, Ontario, Canada

* To whom correspondence should be addressed. E-mail: sehmir{at}mcmaster.ca.

Eosinophil-lineage committed progenitors increase in the bone marrow of asthmatics developing allergen-induced airway hyperresponsiveness and eosinophilia. Also, higher numbers of circulating eosinophil/basophil colony forming units have been demonstrated 24h after allergen inhalation and in bronchial and nasal biopsies of allergic individuals. These cells may undergo in-situ eosinophilopoiesis, suggesting that following allergen inhalation, progenitor cells traffic from the bone marrow to the airways providing an ongoing source of effector cells. To examine this possibility, CD34+ and CD34+IL-5R{alpha}+ cells were measured in induced sputum from allergic asthmatics at baseline, 7 and 24h following allergen and diluent inhalation, using flow cytometry. Isolated early responders (n=9) were contrasted to dual responders (n=9), who develop allergen-induced sputum and blood eosinophilia and airway hyperresponsiveness, and to normal controls. At baseline, there were significantly fewer sputum eosinophils and CD34+ cells in normals compared to asthmatics. Sputum CD34+ cells increased at 7h following allergen inhalation in both groups of asthmatics, which was sustained at 24h in the dual responder group only, associated with sustained increases in sputum CD34+IL-5R{alpha}+ cells, eosinophils and IL-5. These results indicate that eosinophil progenitors can migrate to the airways and may differentiate towards an eosinophilic phenotype.
Key words: Stem Cell Mobilization, Asthma, Airway




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
H. H. Kariyawasam, M. Aizen, J. Barkans, D. S. Robinson, and A. B. Kay
Remodeling and Airway Hyperresponsiveness but Not Cellular Inflammation Persist after Allergen Challenge in Asthma
Am. J. Respir. Crit. Care Med., May 1, 2007; 175(9): 896 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
J. A. Denburg and S. F. van Eeden
Bone marrow progenitors in inflammation and repair: new vistas in respiratory biology and pathophysiology.
Eur. Respir. J., March 1, 2006; 27(3): 441 - 445.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. Fabbri, S. P. Peters, I. Pavord, S. E. Wenzel, S. C. Lazarus, W. MacNee, F. Lemaire, and E. Abraham
Allergic Rhinitis, Asthma, Airway Biology, and Chronic Obstructive Pulmonary Disease in AJRCCM in 2004
Am. J. Respir. Crit. Care Med., April 1, 2005; 171(7): 686 - 698.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. J. Sterk and P. S. Hiemstra
Eosinophil Progenitors in Sputum: Throwing out the Baby with the Bath Water?
Am. J. Respir. Crit. Care Med., March 1, 2004; 169(5): 549 - 550.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2003 American Thoracic Society 		  ATS Quiz on Sleep Study Tracings