help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on September 11, 2003, doi:10.1164/rccm.200306-837OC

Am. J. Respir. Crit. Care Med., Volume 168, Number 11, December 2003, 1346-1352

A more recent version of this article appeared on December 1, 2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200306-837OCv1
168/11/1346    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lewinsohn, D. A
Right arrow Articles by Lewinsohn, D. M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lewinsohn, D. A
Right arrow Articles by Lewinsohn, D. M

Submitted on June 26, 2003
Accepted on September 9, 2003

Mycobacterium tuberculosis -specific CD8+ T cells preferentially recognize heavily infected cells

Deborah A Lewinsohn1*, Amy S Heinzel2, James M Gardner3, Liqing Zhu4, Mark R Alderson4, and David M Lewinsohn5

1 Division of Infectious Diseases and Pediatrics, Oregon Health & Science University, Portland, Oregon, USA; Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon, USA; Vaccine and Gene Therapy Center, Oregon Health & Science University, Portland, Oregon, USA, 2 Division of Pulmonary and Critical Medicine and Department of Medicine, Oregon Health & Science University, Portland, Oregon, USA; Medicine, Portland VA Medical Center, Portland, Oregon, USA, 3 Division of Infectious Diseases and Pediatrics, Oregon Health & Science University, Portland, Oregon, USA, 4 Immunology, Corixa Corporation, Seattle, Oregon, USA, 5 Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon, USA; Vaccine and Gene Therapy Center, Oregon Health & Science University, Portland, Oregon, USA; Division of Pulmonary and Critical Medicine and Department of Medicine, Oregon Health & Science University, Portland, Oregon, USA

* To whom correspondence should be addressed. E-mail: lewinsde{at}ohsu.edu.

Both CD4+ and CD8+ T cells are important for successful immunity to tuberculosis and have redundant effector functions, such as cytolysis and release of potent anti-mycobacterial cytokines such as interferon-{gamma} and tumor necrosis factor-{alpha}. We hypothesized that CD8+ T cells play a unique role in host defense to Mycobacterium tuberculosis infection as well. Possibilities include preferential and/or enhanced release of granular constituents and/or preferential recognition of heavily infected cells. Utilizing human, Mycobacterium tuberculosis-specific, CD4+ and CD8+ T cell clones, we demonstrate that, following recognition of antigen presenting cells displaying peptide antigen, CD4+ T cells preferentially release IFN-{gamma}, whereas CD8+ T cells preferentially lyse antigen presenting cells. Furthermore, utilizing dendritic cells infected with Mycobacterium tuberculosis expressing green fluorescent protein, we show that CD8+ T cells preferentially recognize heavily infected cells that constitute the minority of infected cells. These data support the hypothesis that the central role of CD8+ T cells in the control of infection with Mycobacterium tuberculosis may be that of surveillance; in essence, recognition of cells in which the containment of Mycobacterium tuberculosisis no longer effective.
Key words: CD8-Positive T-Lymphocytes; CD4-Positive T-Lymphocytes; Cytotoxic T-Lymphocytes; Antigen Presentation




This article has been cited by other articles:


Home page
 ThoraxHome page
I Petrache, K Diab, K S Knox, H L Twigg III, R S Stephens, S Flores, and R M Tuder
HIV associated pulmonary emphysema: a review of the literature and inquiry into its mechanism
Thorax, May 1, 2008; 63(5): 463 - 469.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Billeskov, C. Vingsbo-Lundberg, P. Andersen, and J. Dietrich
Induction of CD8 T Cells against a Novel Epitope in TB10.4: Correlation with Mycobacterial Virulence and the Presence of a Functional Region of Difference-1
J. Immunol., September 15, 2007; 179(6): 3973 - 3981.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. M. Lewinsohn, J. E. Grotzke, A. S. Heinzel, L. Zhu, P. J. Ovendale, M. Johnson, and M. R. Alderson
Secreted Proteins from Mycobacterium tuberculosis Gain Access to the Cytosolic MHC Class-I Antigen-Processing Pathway
J. Immunol., July 1, 2006; 177(1): 437 - 442.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. Carranza, E. Juarez, M. Torres, J. J. Ellner, E. Sada, and S. K. Schwander
Mycobacterium tuberculosis Growth Control by Lung Macrophages and CD8 Cells from Patient Contacts
Am. J. Respir. Crit. Care Med., January 15, 2006; 173(2): 238 - 245.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
I. Smith, C. Nathan, and H. H. Peavy
Progress and New Directions in Genetics of Tuberculosis: An NHLBI Working Group Report
Am. J. Respir. Crit. Care Med., December 15, 2005; 172(12): 1491 - 1496.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Lazarevic, D. Nolt, and J. L. Flynn
Long-Term Control of Mycobacterium tuberculosis Infection Is Mediated by Dynamic Immune Responses
J. Immunol., July 15, 2005; 175(2): 1107 - 1117.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Shams, P. Klucar, S. E. Weis, A. Lalvani, P. K. Moonan, H. Safi, B. Wizel, K. Ewer, G. T. Nepom, D. M. Lewinsohn, et al.
Characterization of a Mycobacterium tuberculosis Peptide That Is Recognized by Human CD4+ and CD8+ T Cells in the Context of Multiple HLA Alleles
J. Immunol., August 1, 2004; 173(3): 1966 - 1977.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. Tobin
Tuberculosis, Lung Infections, Interstitial Lung Disease, Social Issues and Journalology in AJRCCM 2003
Am. J. Respir. Crit. Care Med., January 15, 2004; 169(2): 288 - 300.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2003 American Thoracic Society 		  ATS PAR News