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Published ahead of print on December 4, 2003, doi:10.1164/rccm.200305-663OC

Am. J. Respir. Crit. Care Med., Volume 169, Number 4, February 2004, 494-498

A more recent version of this article appeared on February 15, 2004
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Submitted on May 23, 2003
Accepted on November 24, 2003

Regulation of mite allergen pulsed murine dendritic cells by respiratory syncytial virus

Yuki Kondo1, Hiroto Matsuse1*, Ikuko Machida1, Tetsuya Kawano1, Sachiko Saeki1, Shinya Tomari1, Yasushi Obase1, Chizu Fukushima1, and Shigeru Kohno1

1 Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan

* To whom correspondence should be addressed. E-mail: hmatsuse{at}net.nagasaki-u.ac.jp.

Dendritic cells (DCs) are the only antigen-presenting cells that determine T cell differentiation and play an important role in both allergy and viral infection. Respiratory syncytial virus (RSV) can infect DCs and affect their functions. The aim of the present study was to determine the interaction between RSV infection and Dermatophagoides farinae (Df) allergen sensitization on the development of allergy at DC level. Murine bone marrow-derived DCs were prepared and treated as Control, Df-pulsed, UV-inactivated RSV (UVRSV)-challenged, RSV-infected, Df-pulsed plus UVRSV-challenged, Df-pulsed plus RSV-infected. In in vitro experiments, we compared the expression of costimulatory molecules and cytokine production between the six groups of DCs. These DCs were then intranasally inoculated into another group of naive mice, followed by intranasal challenge with Df allergen to develop allergic airway inflammation in vivo. In comparison with Df-DCs, Df-RSV-DCs showed Th1-favored expression of costimulatory molecules and cytokine production. Allergic airway inflammation induced by intranasal instillation of Df-DCs was abrogated when infected with RSV, which was associated with a concomitant suppression of Th2 response in the lung. Our results indicated that RSV suppresses Df-DCs to induce Th2 response both in vitro and in vivo through regulation of expression of surface markers and production of immunoregulatory cytokines.
Key words: Dermatophagoides farinae, animal model, cytokine, asthma




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