Published ahead of print on March 4, 2004, doi:10.1164/rccm.200211-1278OC Am. J. Respir. Crit. Care Med., Volume 169, Number 10, May 2004, 1135-1143 A more recent version of this article appeared on May 15, 2004
Submitted on November 26, 2002 Differential Gene Expression in Gram-Negative and Gram-Positive SepsisSung-Liang Yu1,1 Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, 2 Department of Medical Research, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, 3 Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, 4 Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, 5 Department of Medical Research, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan; Institute of Biomedical Sciences and Molecular Biology, National Chung Hsing University, Taichung, Taiwan; Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, 6 Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan; Department of Medical Research, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan * To whom correspondence should be addressed. E-mail: fylin1{at}ha.mc.ntu.edu.tw.
Sepsis is the most common cause of death in patients in the intensive care unit. Genome-wide gene expression analysis can provide insights into the molecular alterations of sepsis. In following, total mRNA was extracted from the livers of six uninfected control mice and 60 septic mice following infusion of either live E. coli or S. aureus. Using a murine cDNA microarray system, changes in gene expression were monitored at 6 time points (uninfected, 2, 8, 24, 48, and 72 hours). Overall, 4.8% of 6,144 assessed genes were differentially regulated with a greater than two-fold change across all time points. Most of the genes with altered expression were commonly present in gram-negative and gram-positive sepsis, but the expressions of 17 genes were different between both types of sepsis at particular time points after infection. The microarray results support the hypothesis that both gram-positive and gram-negative sepsis share a final common pathway involved in the pathogenesis of sepsis, but certain genes are differentially expressed under distinct regulation. These results may provide insights into the pathogenesis of sepsis and may also help identify some altered genes that can serve as new targets for diagnostic tools and therapeutic strategies. Key words: microarray, liver, bacterial infection
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