help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on December 12, 2002, doi:10.1164/rccm.200208-889OC

Am. J. Respir. Crit. Care Med., Volume 167, Number 8, April 2003, 1057-1063

A more recent version of this article appeared on April 15, 2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
200208-889OCv1
167/8/1057    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gajic, O.
Right arrow Articles by Hubmayr, R. D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gajic, O.
Right arrow Articles by Hubmayr, R. D

Submitted on August 21, 2002
Accepted on December 9, 2002

Ventilator-Induced Cell Wounding and Repair in the Intact Lung

Ognjen Gajic1*, Jaeho Lee1, Clinton H Doerr1, Jorge C Berrios1, Jeffrey L Myers2, and Rolf D Hubmayr3

1 Thoracic Diseases Research Unit, Division of Pulmonary and Critical Care Medicine, Department of Medicine, Mayo Clinic, Rochester, MN, USA, 2 Department of Pathology, Mayo Clinic, Rochester, MN, USA, 3 Thoracic Diseases Research Unit, Division of Pulmonary and Critical Care Medicine, Department of Medicine, Mayo Clinic, Rochester, MN, USA; Department of Physiology & Biophysics, Mayo Clinic, Rochester, MN, USA

* To whom correspondence should be addressed. E-mail: gajic.ognjen{at}mayo.edu.

We tested the hypothesis that cells of ventilator-injured lungs are subject to reversible plasma membrane stress failure. Rat lungs were perfused with the membrane impermeable fluorescent marker propidium iodide and randomized to one of four ventilation strategies. Subpleural lung regions were imaged with confocal microscopy and cell injury quantified as the number of propidium iodide positive cells per alveolus. The number of injured cells was significantly greater in lungs ventilated with large tidal volumes and zero end-expiratory pressure than in lungs ventilated with small tidal volumes and positive end-expiratory pressure (p < 0.01). Cell injury correlated with lung weight gain, change in dynamic compliance, and histologic injury scores. In a second set of experiments, lungs were mechanically ventilated for 30 minutes at high tidal volume settings while propidium iodide was perfused either during or after injurious ventilation. Labeling after removal of injurious stress revealed significantly fewer injured cells (0.25 ± 0.09 to 0.08 ± 0.08, p < 0.01). We conclude that cells of ventilator-injured lungs are subject to reversible plasma membrane stress failure.
Key words: ventilator-induced lung injury, plasma membrane resealing, confocal microscopy, isolated perfused rat lung, stress failure




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. P. Desai, K. E. Chapman, and C. M. Waters
Mechanical stretch decreases migration of alveolar epithelial cells through mechanisms involving Rac1 and Tiam1
Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L958 - L965.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
T. R. Martin
Interactions between Mechanical and Biological Processes in Acute Lung Injury
Proceedings of the ATS, April 15, 2008; 5(3): 291 - 296.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
R. A. Oeckler and R. D. Hubmayr
Ventilator-associated lung injury: a search for better therapeutic targets
Eur. Respir. J., December 1, 2007; 30(6): 1216 - 1226.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. Liu, Y. Hotta, A. R. Graveline, O. V. Evgenov, E. S. Buys, K. D. Bloch, F. Ichinose, and W. M. Zapol
Congenital NOS2 deficiency prevents impairment of hypoxic pulmonary vasoconstriction in murine ventilator-induced lung injury
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1300 - L1305.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. E. Sinclair, R. C. Molthen, S. T. Haworth, C. A. Dawson, and C. M. Waters
Airway Strain during Mechanical Ventilation in an Intact Animal Model
Am. J. Respir. Crit. Care Med., October 15, 2007; 176(8): 786 - 794.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. E. Perlman and J. Bhattacharya
Alveolar expansion imaged by optical sectioning microscopy
J Appl Physiol, September 1, 2007; 103(3): 1037 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Fisher and S. S. Margulies
Modeling the effect of stretch and plasma membrane tension on Na+-K+-ATPase activity in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L40 - L53.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Frank, C. M. Wray, D. F. McAuley, R. Schwendener, and M. A. Matthay
Alveolar macrophages contribute to alveolar barrier dysfunction in ventilator-induced lung injury
Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1191 - L1198.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. B. Allen, B. T. Suratt, L. Rinaldi, J. M. Petty, and J. H. T. Bates
Choosing the frequency of deep inflation in mice: balancing recruitment against ventilator-induced lung injury
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L710 - L717.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Naire and O. E. Jensen
Epithelial cell deformation during surfactant-mediated airway reopening: a theoretical model
J Appl Physiol, August 1, 2005; 99(2): 458 - 471.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. B. Allen, L. A. Pavone, J. D. DiRocco, J. H. T. Bates, and G. F. Nieman
Pulmonary impedance and alveolar instability during injurious ventilation in rats
J Appl Physiol, August 1, 2005; 99(2): 723 - 730.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. Caironi, F. Ichinose, R. Liu, R. C. Jones, K. D. Bloch, and W. M. Zapol
5-Lipoxygenase Deficiency Prevents Respiratory Failure during Ventilator-induced Lung Injury
Am. J. Respir. Crit. Care Med., August 1, 2005; 172(3): 334 - 343.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. E. Vlahakis and R. D. Hubmayr
Cellular Stress Failure in Ventilator-injured Lungs
Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1328 - 1342.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. H. Doerr, O. Gajic, J. C. Berrios, S. Caples, M. Abdel, J. F. Lymp, and R. D. Hubmayr
Hypercapnic Acidosis Impairs Plasma Membrane Wound Resealing in Ventilator-injured Lungs
Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1371 - 1377.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. L. Fisher, I. Levitan, and S. S. Margulies
Plasma Membrane Surface Increases with Tonic Stretch of Alveolar Epithelial Cells
Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2): 200 - 208.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. Tobin
Critical Care Medicine in AJRCCM 2003
Am. J. Respir. Crit. Care Med., January 15, 2004; 169(2): 239 - 253.
[Full Text] [PDF]

Home page
 Eur Respir JHome page
L. Gattinoni, E. Carlesso, P. Cadringher, F. Valenza, F. Vagginelli, and D. Chiumello
Physical and biological triggers of ventilator-induced lung injury and its prevention
Eur. Respir. J., November 16, 2003; 22(47_suppl): 15s - 25s.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. L. McNeil
Cell Suffering and Its Prevention in Lung
Am. J. Respir. Crit. Care Med., April 15, 2003; 167(8): 1046 - 1047.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2002 American Thoracic Society 		  ATS State of the Art Course 2008