Published ahead of print on February 5, 2003, doi:10.1164/rccm.200207-654OC Am. J. Respir. Crit. Care Med., Volume 167, Number 8, April 2003, 1131-1138 A more recent version of this article appeared on April 15, 2003
Submitted on July 4, 2002 Biologically Active ICAM-1 is Shed as Dimers by a Regulated Mechanism in the Inflamed Pleural SpaceMario Melis1,1 Istituto di Fisiopatologia Respiratoria, Consiglio Nazionale delle Ricerche, Palermo, Italy, 2 Istituto di Medicina Generale e Pneumologia, Universita degli Studi di Palermo, Palermo, Italy, 3 Departments of Microbiology and Infectious Diseases and Internal Medicine, University of Calgary, Calgary, Alberta, Canada * To whom correspondence should be addressed. E-mail: marco{at}ifr.pa.cnr.it.
ICAM-1 is an adhesion molecule that plays a crucial role in cell-cell interactions involved in the recruitment of cells and immune responses. Under some circumstances, ICAM-1 is found as a soluble protein that has the potential to influence the nature of immunoinflammatory responses. By examining cells and fluid from the pleural compartment of patients with cancer, tuberculosis and congestive heart failure, the cellular source, conformation, control, and biological activity of soluble ICAM-1 (sICAM-1) was investigated. The results suggest that dimeric sICAM-1 was released locally in the pleural compartment of tuberculous and malignant effusions. sICAM-1, was shed from preexpressed surface ICAM-1 rather than produced de novo, and both CD45-positive leukocytes and cytokeratin-positive epithelial and mesothelial cells expressed ICAM-1 suggesting multiple cellular sources for sICAM-1. The expression of sICAM-1 was regulated since pleural macrophages caused release of sICAM-1 via a TNF- Key words: Pleural effusion; ICAM-1; Mononuclear phagocyte; Lung cancer; Tuberculosis.
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-dependent mechanism. The functional significance of sICAM-1 was demonstrated by showing that pleural sICAM-1 interfered with the conjugate formation between LAK cells and K562 cells, suggesting that pleural sICAM-1 plays an immunosuppressive role by inhibiting adhesion of cytotoxic lymphocytes and tumor cells. Thus, sICAM-1 is shed from the surface of cells in a regulated manner and has the potential to influence the immune response in the pleural space.
