Published ahead of print on December 18, 2002, doi:10.1164/rccm.200206-589OC Am. J. Respir. Crit. Care Med., Volume 167, Number 7, April 2003, 991-998 A more recent version of this article appeared on April 1, 2003
Submitted on July 31, 2002 TA-19, a novel protein antigen of Trichosporon asahii, in summer-type hypersensitivity pneumonitisYasujiro Matsunaga1,1 Pulmonary Medicine, Tokyo Medical and Dental University, Tokyo, Japan, 2 Pulmonary Medicine, Tokyo Medical and Dental University, Tokyo, Japan; Internal Medicine, Tokyo Metropolitan Bokutoh Hospital, Tokyo, Japan * To whom correspondence should be addressed. E-mail: usuibokutoh{at}hotmail.com.
The most common form of hypersensitivity pneumonitis (HP) in Japan is summer-type hypersensitivity pneumonitis (SHP), caused by the inhalation of Trichosporon asahii or Trichosporon mucoides. To seek protein antigens relevant to the immunopathogenesis of SHP, we constructed a cDNA expression library of Trichosporon asahii, a major causative yeast species of SHP. Using the immunoscreening method, we identified and cloned a novel gene encoding a 19-kDa protein, named TA-19, that proved to be specifically recognized in the bronchoalveolar lavage (BAL) fluids and sera of patients with SHP. IgG, IgA, and IgM antibodies to the recombinant TA-19 protein were significantly elevated in the sera as well as in the BAL fluids from SHP patients compared to those from non-SHP groups. This protein also induced SHP-specific proliferation of the mononuclear cells from both the peripheral blood and BAL. These results reveal that TA-19 derived from Trichosporon asahii may play a relevant role in specific cellular and humoral immune responses in patients with SHP. Key words: cDNA expression library, Antigen-specific antibody, Lymphocyte proliferation assay, Bronchoalveolar lavage, G-X-X-X-Q-X-W motif
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