Published ahead of print on October 31, 2002, doi:10.1164/rccm.200204-293OC
American Journal of Respiratory and Critical Care Medicine Vol 167. pp. 158-163, (2003)
© 2003 American Thoracic Society
Increasing Concentration of Inhaled Saline with or without Amiloride
Effect on Mucociliary Clearance in Normal Subjects
Namita Sood,
William D. Bennett,
Kirby Zeman,
James Brown,
Carla Foy,
Richard C. Boucher and
Michael R. Knowles
Division of Pulmonary and Critical Care Medicine, Cystic Fibrosis/Pulmonary Research Center, and Center for Environmental Medicine and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; and Division of Pulmonary and Critical Care Medicine, Ohio State University, Columbus, Ohio
Correspondence and requests for reprints should be addressed to Namita Sood, M.D., Division of Pulmonary and Critical Care Medicine, Ohio State University, 201, Heart & Lung Research Institute, 473 West 12th Avenue, Columbus, OH 43210. E-mail: sood-1{at}medctr.osu.edu
Mucociliary clearance is determined by ciliary activity and rheology of airway surface liquid. To test the hypothesis that mucociliary clearance would increase after inhalation of an osmotically active agent that would increase the volume of airway surface liquid, we measured mucociliary clearance in 16 normal subjects after inhalation of varying tonicities of saline alone, and after pretreatment with a Na+ channel blocker (amiloride). Subjects inhaled vehicle (0.12% saline) or amiloride, followed by inhalation of 0.12, 0.9, or 7% saline. Subsequently, mucociliary clearance rates were measured by  scintigraphy of inhaled 99mTc Fe2O3. Mucociliary clearance of whole and peripheral lung was increased (approximately twofold) after inhalation of increasing concentrations of saline (p < 0.04). Pretreatment with amiloride increased mucociliary clearance rates (approximately twofold) after inhalation of 0.12 and 0.9% saline (p < 0.05), but not 7% saline. The rates of mucociliary clearance by pretreatment with amiloride and 7% saline alone (approximately 1.4% per minute) approached the rapid mucociliary clearance rates (approximately 2.0% per minute) reported in systemic pseudohypoaldosteronism, which has loss-of-function mutations of the epithelial Na+ channel and an increased volume of airway surface liquid. We conclude that maneuvers that increase the volume of airway surface liquid are associated with increased rates of mucociliary clearance in normal subjects.
Key Words: mucociliary clearance • airway surface liquid • epithelial Na+ channel • aerosolized NaCl
This article has been cited by other articles:
|
|
|
|
 
M. Harris, D. Firsov, G. Vuagniaux, M. J. Stutts, and B. C. Rossier
A Novel Neutrophil Elastase Inhibitor Prevents Elastase Activation and Surface Cleavage of the Epithelial Sodium Channel Expressed in Xenopus laevis Oocytes
J. Biol. Chem.,
January 5, 2007;
282(1):
58 - 64.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. H. Levin, S. Sullivan, D. Nielson, B. Yang, W. E. Finkbeiner, and A. S. Verkman
Hypertonic Saline Therapy in Cystic Fibrosis: EVIDENCE AGAINST THE PROPOSED MECHANISM INVOLVING AQUAPORINS
J. Biol. Chem.,
September 1, 2006;
281(35):
25803 - 25812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. H. Donaldson, W. D. Bennett, K. L. Zeman, M. R. Knowles, R. Tarran, and R. C. Boucher
Mucus Clearance and Lung Function in Cystic Fibrosis with Hypertonic Saline
N. Engl. J. Med.,
January 19, 2006;
354(3):
241 - 250.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Hirsh, J. R. Sabater, A. Zamurs, R. T. Smith, A. M. Paradiso, S. Hopkins, W. M. Abraham, and R. C. Boucher
Evaluation of Second Generation Amiloride Analogs as Therapy for Cystic Fibrosis Lung Disease
J. Pharmacol. Exp. Ther.,
December 1, 2004;
311(3):
929 - 938.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Tobin
Pediatrics, Surfactant, and Cystic Fibrosis in AJRCCM 2003
Am. J. Respir. Crit. Care Med.,
January 15, 2004;
169(2):
277 - 287.
[Full Text]
[PDF]
|
|
|
Copyright © 2003 American Thoracic Society
|
|
|
