Published ahead of print on May 16, 2007, doi:10.1164/rccm.200611-1722OC
© 2007 American Thoracic Society doi: 10.1164/rccm.200611-1722OC
Chronic Alcoholism Alters Systemic and Pulmonary Glutathione Redox Status1 Division of Neonatology, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia; and 2 Division of Pulmonary and Critical Care, Department of Medicine, University of Colorado at Denver and Health Sciences Center, Denver, Colorado Correspondence and requests for reprints should be addressed to Lou Ann S. Brown, Ph.D., Department of Pediatrics, Emory University, 2015 Uppergate Drive, Atlanta, GA 30322. E-mail: lbrow03{at}emory.edu
Rationale: Previous studies have linked the development and severity of acute respiratory distress syndrome with a history of alcohol abuse. In clinical studies, this association has been centered on depletion of pulmonary glutathione and subsequent chronic oxidant stress. Objectives: The impact on redox potential of the plasma or pulmonary pools, however, has never been reported. Methods: Plasma and bronchoalveolar lavage fluid were collected from otherwise healthy alcohol-dependent subjects and control subjects matched by age, sex, and smoking history. Measurements and Main Results: Redox potential was calculated from measured reduced and oxidized glutathione in plasma and lavage. Among subjects who did and did not smoke, lavage fluid glutathione redox potential was more oxidized in alcohol abusers by approximately 40 mV, which was not altered by dilution. This oxidation of the airway lining fluid associated with chronic alcohol abuse was independent of smoking history. A shift by 20 mV in plasma glutathione redox potential, however, was noted only in subjects who both abused alcohol and smoked. Conclusions: Chronic alcoholism was associated with alveolar oxidation and, with smoking, systemic oxidation. However, systemic oxidation did not accurately reflect the dramatic alcohol-induced oxidant stress in the alveolar space. Although there was compensation for the oxidant stress caused by smoking in control groups, the capacity to maintain a reduced environment in the alveolar space was overwhelmed in those who abused alcohol. The significant alcohol-induced chronic oxidant stress in the alveolar space and the subsequent ramifications may be an important modulator of the increased incidence and severity of acute respiratory distress syndrome in this vulnerable population.
Key Words: glutathione • alcoholism • oxidative stress • pulmonary
The lungs are constantly exposed to environmental and endogenous oxidants. The ability to neutralize these oxidants is essential to maintain pulmonary health. There are major differences between cellular and extracellular compartments in concentration of the thiol/disulfide systems and the relative redox states. Since Cantin and colleagues' report, published in 1987, on the high glutathione (GSH) concentration in the airspace (more than 20-fold than that in the plasma and over 90% in the reduced form), GSH has been considered a primary antioxidant in the alveolar space (1). Although the alveolar space is exposed to the oxidants present in cigarette smoke, the alveolar GSH pool is increased by 80% with no significant oxidant stress, as evidenced by the minimal shift toward the oxidized moiety oxidized glutathione (GSSG) (1). In the airspace, this high GSH concentration defends the lung against endogenously produced oxidants as well as environmental oxidants. Limited availability of GSH in the alveolar space is associated with a number of pulmonary diseases, including cystic fibrosis, acute respiratory distress syndrome (ARDS), chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis, idiopathic interstitial pneumonia, and diffuse fibrosing alveolitis (2–4).
Previous studies reported a 2.7-fold elevation in the risk for ARDS in otherwise healthy alcohol abusers (5–7). Although the underlying cause remains unclear, recent research has elucidated a number of potential contributors, with one of the major causes being chronic oxidative stress. As outlined in a review by Guidot and Hart (8), the alcoholic lungs in ethanol-fed animal models are under increased oxidative stress, have altered nitric oxide metabolism, express abnormal amounts of transforming growth factor (TGF)-
Subject Enrollment Recruitment of subjects who have been chronically dependent on alcohol was made at the detoxification unit at the Veterans' Affairs Hospital in Atlanta, Georgia. Nonsmoking and smoking control subjects were enrolled from those who replied to postings at the different Emory University hospitals as well as from the community. The details of the recruitment process and selection criteria were previously reported (10). Basically, the alcoholic status was confirmed by a score of greater than 3 on the Short Michigan Alcohol Screening Test (SMAST) survey. Those with a score of 0 were considered as control subjects. Exclusion criteria included subjects with a prior history of cardiac disease, liver dysfunction, kidney disease, diabetes mellitus, lung disease, human immunodeficiency virus infection, gastrointestinal bleeding, or concomitant illicit drug use.
Collection and Processing of BALF
Protein and pH Measurements of BALF
HPLC Measurement of Thiols
Redox Potential Calculations
Statistical Analysis
Patient Demographics As a group, subjects with alcohol dependence were slightly older than the control subjects, although control subjects were recruited by age matching. The distribution in sex did not differ between the groups (Table 1).
Basic Parameters of BALF Samples As reported in Table 2, the median pH and corresponding ranges in the BALF of control subjects who did or did not smoke were 6.55 (6.45–6.83) and 6.51 (6.39–6.63), respectively. These were not different from BALF pH of the alcohol-dependent subjects who also did (6.37 [6.27–6.69]) or did not smoke (6.53 [6.44–6.68]). The urea concentrations in BALF or the dilution factor calculated as previously mentioned did not differ with smoking or alcohol status.
GSH and GSSG Concentrations and Redox Potential in the Alveolar Fluids Previous work in this group reported a decrease in total GSH concentration in the ELF with chronic alcoholism, from 576 to 79 µM (10). In this study, there was an alcohol-associated fourfold decrease in BALF total GSH regardless of smoking status (nonsmokers: 8.3 µM [4.7, 10.1] vs. 2.1 µM [1.2, 3.2]; smokers: 25.2 [7.84, 33.5] vs. 4.6 µM [2.18, 7.26], p < 0.05). The total glutathione pool (GSH + GSSG) in the ELF was also significantly depleted with chronic alcoholism, by eightfold among nonsmokers (400.4 [201.8, 556.7] vs. 53.4 µM [36.1, 68.5], p < 0.05) and twofold among smokers (546.3 [264.8, 818.8] vs. 253.1 µM [55.3, 517.1]). Chronic alcohol abuse was associated with a depletion of BALF GSH by more than 60% in both nonsmokers and smokers (p < 0.002) (Figure 1). The depletion was apparently alcohol dependent because stratifying by smoking status did not result in significant differences. A positive smoking history elevated GSSG by fivefold (0.11 [0.03–0.33] vs. 0.94 µM [0.15–2.05], p = 0.008). A similar increase in BALF GSSG concentration due to smoking was noted among subjects with chronic alcoholism (0.22 [0.05–0.49] vs. 1.02 µM [0.42–2.80]). Because the GSH concentrations decreased and the GSSG concentration increased, the percentage present as the oxidized moiety (%GSSG) was assessed and, in the control groups, found to be twofold higher in smokers than nonsmokers (p = 0.02). Among subjects who abused alcohol, %GSSG was approximately fourfold greater in smokers (Figure 2). Correspondingly, both groups of alcohol-dependent subjects were more oxidized by the %GSSG measure compared with the appropriate control population (with and without a smoking history; p < 0.003).
Despite the increased GSH concentration in the BALF of the smoking control group, the redox potential for the GSH/GSSG pair as calculated from the Nernst equation was not statistically different from values of nonsmoking control subjects (Figure 3). When the two groups of nonsmokers were compared, a history of alcohol abuse resulted in a significantly more oxidized alveolar microenvironment as evidenced by a shift in the GSH/GSSG redox potential of 40 mV in the BALF (p < 0.004). An even greater shift (50 mV) toward an oxidized state associated with alcohol dependence was apparent among smokers (p < 0.001). A history of smoking alone, however, did not alter GSH redox potential of subjects with chronic alcoholism.
To consider conditions more representative of the ELF, redox potential for GSH was calculated after the dilution of the GSH and GSSG pools by the lavage procedure was corrected by the urea method. As observed in the BALF, the Eh for the GSH/GSSG pair in ELF was not statistically different in the control groups (i.e., not altered by a smoking history) (Figure 4). When the two nonsmoking groups were compared, a history of alcohol abuse resulted in a 50-mV shift in the ELF toward a more oxidized state (p < 0.01). A similar pattern was observed in the two smoking groups, with a 45-mV shift toward an oxidized state in those subjects who abused alcohol (p < 0.01). As observed in the BALF, there was minimal change in the redox pair within the alcohol-independent or alcohol-dependent groups when stratified according to their smoking history.
If we assume that the pH of the ELF could be estimated by multiplying the BALF H+ ion (derived by BALF pH) with the urea dilution factor, then, indeed, the low ELF pH leads to a shift in redox potential toward the more oxidized state, from a median of –170 to –50 mV for nonsmoking control subjects. However, a similar shift applies to the other groups as well. Therefore, the differential in the redox potential values between alcoholics and their respective smoking control subjects remained the same and was independent of whether the dilution of pH was adjusted. The effect of alcoholism was still associated with an approximate 30-mV shift of redox potential toward the more oxidized state: 1.0 versus –51.9 mV among nonsmokers and –10.1 versus –39.1 mV among smokers.
GSH and GSSG Concentrations and Redox Potential in Plasma
This oxidative shift was not detected by merely comparing plasma GSH concentration (Figure 6). Median plasma GSH concentrations were not statistically different at 2.9 (0.5–4.8) and 3.6 µM (2.0–6.4), respectively, for subjects who did or did not abuse alcohol. Even among smokers, chronic alcohol dependence did not significantly decrease plasma GSH (2.4 [1.4–4.6] vs. 4.1 µM [3.4–6.8]). Alcohol abusers, regardless of smoking status, had similar plasma GSH levels.
Systemic GSH oxidant status was also evaluated in terms of %GSSG, calculated as the percentage of the total plasma GSH present as GSSG. A history of alcohol abuse was associated with increased %GSSG, by approximately 1% among nonsmokers, and aggravated by the combination of smoking and alcohol dependence to more than threefold that of smokers (p = 0.02) (Figure 7). As was the case for both Eh (GSH) and GSH concentration, %GSSG did not differ by smoking status among subjects with a history of alcoholism.
Alcohol is the most frequently abused drug in the world, claiming 100,000 lives annually and costing more than $100 billion per year in alcohol-related problems (17). In the United States, 15 to 20 million individuals are classified as alcoholics, and in patients admitted to general hospitals, 20 to 40% have alcohol-related disorders (17). In trauma patients, the risk of pulmonary complications, such as respiratory failure requiring mechanical ventilation, is higher if there is a history of alcohol abuse (7). In septic shock patients, 70% of alcohol-dependent patients developed ARDS compared with 31% among others. After controlling for potential confounding variables, the relative risk of ARDS associated with chronic alcohol abuse was 3.7 (6). In these studies, a history of alcohol abuse alone did not result in acute lung injury. Rather, alcohol abuse increased the incidence and severity of ARDS when superimposed on a second injury, such as trauma, sepsis, or aspiration. Several studies indicated that changes in plasma GSH and GSSG concentrations or deviation from a redox potential of approximately –140 mV provided a measure of systemic oxidant stress (18–20). In the current study, the plasma GSH, %GSSG, and the redox potential for the GSH/GSSG thiol pair of nonsmoking control subjects were similar to those reported elsewhere. Studies by Jones and coworkers (21) demonstrated a 11-mV shift in the redox potential toward a more oxidized state in subjects who had a smoking history. That oxidation of the plasma pool in subjects who smoke was not observed in the current study as measured by decreased GSH, increased GSSG, or changes in the GSH/GSSSG redox potential. It is unclear why different results were obtained. However, it may due to sample size because the current study was not powered to detect significance in an 11-mV change in the redox potential. In addition, the study by Samiec and colleagues (20) reported an age-related increase in the GSH/GSSG redox potential of 0.7 mV per year after age 45. In the current study, the subjects ranged in age from 34 to 56 years, a factor that could also contribute to variance in the redox potential.
In a study by Loguercio and coworkers (22) that included 10 subjects with chronic alcohol abuse, depletion of plasma GSH has previously been reported. The current study did not display the same effects of alcohol on plasma GSH levels. Concurrent alcohol abuse and smoking significantly increased the percentage of the glutathione pool present as GSSG. This increase in plasma %GSSG was mirrored in smoking alcoholics by a similar shift in the redox potential of the GSH/GSSG pair toward an oxidized state ( As observed by others (1), the GSH concentration in the ELF was 140-fold greater than that present in the plasma, with less than 5% of the ELF pool present as the oxidized moiety. After correction for dilution by the lavage procedure, the redox potential of the ELF was approximately –190 mV, a redox potential that approaches that of tissues (–185 to –258 mV) (24). When the GSH/GSSG redox potential of the plasma and the ELF were compared, the ELF was 30 mV more reduced than plasma. In the BALF, a smoking history is associated with a significant increase in the GSH pool, with 98% remaining in the reduced form (1). In the current study, a similar increase in lavage fluid GSH concentration was suggested in smoking control subjects. However, this smoking-associated increase in GSH was accompanied by an increase in GSSG. As a result, smoking was not associated with a significant shift in the GSH/GSSG redox potential in the BALF or in the ELF of the control groups. In previous studies by this research group, a history of alcohol abuse was associated with an 80% decreased in the GSH content of the lavage fluid and a fourfold increase in the %GSSG (10, 11). In the current study, a similar decrease in the GSH pool was observed in those subjects who abused alcohol. Furthermore, this decrease in GSH associated with alcohol abuse was independent of their smoking history. When the GSSG pool was assessed, alcohol abuse resulted in a three- to fourfold increase in those subjects, independent of their smoking history. When the GSH/GSSG redox potential of the BALF or the ELF was determined, the alveolar lining fluid was approximately 40 mV more oxidized in subjects with a history of alcoholism. This dramatic increase in alveolar oxidant stress was independent of their smoking histories. Therefore, the ELF redox potential of subjects who abused alcohol was only 15 mV more reduced than the corresponding plasma sample. This was in sharp contrast to a 30-mV ELF-to-plasma potential difference observed in control subjects.
In the alveolar space, GSH protects by neutralizing environmentally or endogenously derived free radical species. As a major antioxidant in the alveolar space, it is used not only to eliminate peroxides but to also maintain vitamins C and E in their reduced and functional forms (25). Alveolar epithelial cells and airspace cells are dependent on the availability of GSH in the ELF and dramatic decreases could promote intracellular oxidant stress and negatively impact cellular functions and cell viability. Oxidant stress can promote lipid peroxidation, protein cross-linking, protein fragmentation, and DNA damage and strand breaks. The effects of the extracellular GSH redox state on cell apoptosis, nuclear factor (NF)- The redox potential of GSH in plasma was approximately 18 mV more oxidized in subjects with chronic alcoholism compared with age-, sex-, and smoking-matched control subjects. This suggested that a history of alcohol abuse was associated with systemic oxidant stress, which was not detectable in GSH measurements alone. The shift in redox potential toward the more oxidized state was significantly greater in the BALF and the ELF. Thus, the changes in the redox potential of the plasma underestimated the alcohol-induced changes in the redox potential of the alveolar space. When comparing GSH or GSSG concentrations in the BALF, there is a need to establish a valid normalization for lavage dilution. Similarly, the determination of the actual redox potential of the GSH/GSSG pair in the ELF is dependent on correction for the dilution due to the lavage procedure. However, the GSH/GSSG redox potential of the BALF and the ELF both shifted by 40 mV in the otherwise healthy subjects with a history of alcoholism. In other words, a normalization factor was not needed to determine the degree by which decreases in GSH and increases in GSSG translated into oxidant stress in the alveolar space. Rather, determination of the redox state of the GSH/GSSG thiol pair provided a dynamic and quantitative measure of oxidant stress in the alveolar space (16). There are undoubtedly multiple mechanisms that could potentially contribute to the altered balance between GSH and GSSG, including increased cellular uptake and utilization. Our data represent only the free GSH and GSSG, and decreased GSH could be partially explained by a shift from free to protein-bound GSH. Of course, chronic alcoholism may alter the activity of enzymes that affect GSH and GSSG concentration and balance (e.g., glutathione reductase and glutathione peroxidase). In addition, increased utilization for the detoxification of other oxidants could account for this decrease in the GSH pool. Human studies undoubtedly require consideration of a large number of factors that may contribute to the observed findings. In these subjects, there are a number of dietary factors that may alter plasma thiol state, including sulfur amino acid intake, availability of glutamine, dietary antioxidants, inducers of GSH synthesis, and redox active micronutrients such as selenium, flavin, and niacin (39). Chronic alcoholism has been associated with deficiency in folate and other B vitamins that may participate in methionine and cysteine synthesis (40, 41). Specific transporters move extracellular amino acids into the cells, and although studies in alcoholic micropig models have suggested down-regulation of transporters involved in folate and B-vitamin absorption, the effects of alcohol on these mechanisms in the lung remain unstudied. Evaluations of these numerous contributors in human studies would better explain the observations and reveal effective targets to decrease the risk of ARDS in this vulnerable population. In summary, individuals with a history of chronic alcohol abuse manifest significant oxidant stress in the alveolar space as defined by decreased GSH, increased GSSG, and an oxidative shift in the redox potential. The degree of alcohol-induced oxidant stress in the alveolar space was not accurately reflected systemically. Because the oxidant stress in the ELF was not exacerbated by a smoking history, alcohol abuse was suggested as the driving factor in alveolar GSH depletion and oxidant stress. In the smoking control groups, there was compensation for the oxidant stress caused by smoking and the redox potential in the alveolar space was not altered. However, the capacity to maintain a reduced GSH pool was overwhelmed in the alveolar space of subjects with long-term alcohol abuse and suggested that history of alcohol abuse resulted in a greater oxidant burden than a history of smoking. Therefore, GSH deficiency and the subsequent ramifications of significant chronic oxidant stress in the alveolar space may explain the increased incidence and severity of ARDS in this vulnerable population.
Supported by the National Institute of Alcohol Abuse and Addiction grants R21 AA015335 (L.A.S.B. and M.M.), R01 AA-014435 (M.M.), and 1 P50 AA135757 (L.A.S.B., M.M., and E.L.B.). Originally Published in Press as DOI: 10.1164/rccm.200611-1722OC on May 16, 2007 Conflict of Interest Statement: None of the authors has a financial relationship with a commercial entity that has an interest in the subject of this manuscript. Received in original form November 28, 2007; accepted in final form May 16, 2007
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ABSTRACT
AT A GLANCE COMMENTARY
, and are in a chronic proinflammatory state, with up-regulated expressions of IL-1
. In this animal model, chronic ethanol ingestion depleted the GSH pool in the alveolar epithelial lining fluid (ELF) by as much as 80% (
-glutamyl-glutamate (
17 mV). Therefore, the significantly more oxidized redox potential of the GSH/GSSG pair and the greater %GSSG suggested a systemic oxidative stress, particularly in alcohol abusers who also smoked, a behavior characteristic of 80% of all alcoholics (
B activation, activator protein (AP)-1 activation, and cell signaling are areas of recent research in pulmonary pathological processes (
