Online Data Supplement to Liver-Lung Interactions Following E. coli Bacteremic Sepsis and Secondary Hepatic Ischemia/Reperfusion InjuryFigure E1. Representative photomicrographs of CAE-stained ischemic liver sections obtained from animals at t = 195 min after bacteremic infection with 109 E. coli serotype O55:B5 or infusion of 0.9% NaCl at t = 0, with and without subsequent liver ischemia and brief (1-h) reperfusion. (a) EC + I/R; (b) EC + Sham; (c) NS + I/R; (d) NS + Sham. Images were obtained using Tri-X Kodak film without filtration at a nominal magnification of x 100. Arrows designate polymorphonuclear leukocytes; asterisks designate vacuoles. Bar = 50 :m. Download Figure E1aDownload Figure E1bDownload Figure E1cDownload Figure E1dFigure E2. Mean values (± SEM) for organ W/D ratios after bacteremic infection with 109 E. coli serotype O55:B5 (EC) or infusion of 0.9% NaCl (NS) at t = 0, with and without subsequent liver ischemia and brief (1-h) reperfusion. *p < 0.05 EC + I/R versus cecal W/D values of EC + Sham, NS + I/R, and NS + Sham groups. Download Figure E2Figure E3. Mean values (± SEM) for organ W/D ratios after bacteremic infection with 109 E. coli serotype O55:B5 or infusion of 0.9% NaCl at t = 0, with and without subsequent liver ischemia and longer-term (24-h) reperfusion. *p < 0.05 versus lung W/D values of EC + Sham, NS + I/R, and NS + Sham groups. Download Figure E3Figure E4. Mean values (± SEM) for rectal temperature after bacteremic infection with 109 E. coli serotype O55:B5 or infusion of 0.9% NaCl at t = 0, with and without subsequent liver ischemia and longer-term (24-h) reperfusion. There were no significant intergroup differences. Download Figure E4Figure E5. Mean values (± SEM) for mean arterial pressure after bacteremic infection with 109 E. coli serotype O55:B5 or infusion of 0.9% NaCl at t = 0, with and without subsequent liver ischemia and longer-term (24-h) reperfusion. There were no significant intergroup differences. Download Figure E5Figure E6. Mean values (± SEM) for pulse rate after bacteremic infection with 109 E. coli serotype O55:B5 or infusion of 0.9% NaCl at t = 0, with and without subsequent liver ischemia and long-term (24-h) reperfusion. There were no significant intergroup differences. Download Figure E6Figure E7. Mean values (± SEM) for respiratory frequency after bacteremic infection with 109 E. coli serotype O55:B5 or infusion of 0.9% NaCl at t = 0, with and without subsequent liver ischemia and longer-term (24-h) reperfusion. *p < 0.05 versus within-group baseline value; Hp < 0.05 versus time-matched NS + Sham value. Download Figure E7These files are in Adobe Acrobat (PDF) format. If you have not installed and configured the Adobe Acrobat Reader on your system, please see Help with Printing for instructions.
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