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ABSTRACT |
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ABSTRACT
INTRODUCTION
METHODS
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DISCUSSION
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The cysteinyl leukotrienes (CysLTs) are important mediators of human asthma. Pharmacologic and clinical studies show that the CysLTs exert most of their bronchoconstrictive and proinflammatory effects through activation of a putative, 7-transmembrane domain, G-protein-coupled receptor, the CysLT1 receptor. The initial molecular characterization of the CysLT1 receptor showed by in situ hybridization, the presence of CysLT1 receptor messenger RNA (mRNA) in human lung smooth-muscle cells and lung macrophages. We confirmed the results of these in situ hybridization analyses for the CysLT1 receptor, and produced the first immunohistochemical characterization of the CysLT1 receptor protein in human lung. The identification of the CysLT1 receptor in the lung is consistent with the antibronchoconstrictive and antiinflammatory actions of CysLT1 receptor antagonists. We also report the expression of CysLT1 receptor mRNA and protein in most peripheral blood eosinophils and pregranulocytic CD34+ cells, and in subsets of monocytes and B lymphocytes.
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INTRODUCTION |
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
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The cysteinyl leukotrienes (CysLTs), leukotriene (LT)C4, LTD4, and LTE4, are produced by LTC4 synthase from the precursor leukotriene LTA4, which is in turn a product of 5-lipoxygenation of the polyunsaturated fatty acid arachidonic acid (1, 2). LTC4 synthase has been shown to be produced by inflammatory cells, and particularly by eosinophils, and the gene for this enzyme is encoded on chromosome 5q13, a locus rich in genes encoding proinflammatory cytokines such as interleukin (IL)-4, IL-5, and IL-13 (3). CysLTs are the most potent bronchoconstrictors known and have been identified in urine and tissues from patients with a number of respiratory diseases, such as asthma, virus-induced wheezing, and bronchial hyperreactivity (4). At least two human CysLT receptors have been defined pharmacologically (8). Most of the biologic activities ascribed to CysLTs, including bronchospasm, plasma exudation, vasoconstriction, mucus secretion, and eosinophil recruitment, are mediated through interaction with the CysLT1 receptor subtype (9). The CysLT1 receptor is also the target of the antiasthmatic CysLT1 receptor antagonists montelukast (Singulair; Merck & Co., West Point, PA) (10), zafirlukast (Accolate; Zeneca Pharmaceuticals, Wilmington, DE) (15), and pranlukast (Onon; SmithKline Beecham, Harlow, UK) (16).
The complementary DNA (cDNA) for the CysLT1 receptor has been recently cloned, and encodes a 337-amino acid, G-protein-coupled receptor (GPCR) putatively spanning the 7-transmembrane domain (17, 18). The major intracellular signaling pathway for activation of the recombinant CysLT1 receptor is via calcium release (17, 18). A second CysLT receptor, CysLT2, has been described pharmacologically and appears to be implicated in some of the vascular effects of CysLTs (19). In the human monocytic THP-1 cell, there are at least two signaling pathways activated by CysLTs, which respectively lead to an increase in intracellular calcium that results in activation of mitogen-activated kinase (MAP kinase), and a pertussis toxin-sensitive chemotactic response pathway (20).
The molecular cloning of the CysLT1 receptor has allowed investigation of the expression of this target for asthma therapy. In Northern blot analyses of human tissues, the highest level of expression of the CysLT1 receptor was found in peripheral blood leukocytes, followed by spleen, lung, pancreas, small intestine, and a number of other tissues (17, 18). Reverse transcription-polymerase chain reaction (RT-PCR) has revealed the mRNA for the receptor in a number of cell lines, including THP-1 cells and the human lymphoblastic U937 cell line (K. R. Lynch, personal communication). RT-PCR showed no difference in overall lung expression of the receptor mRNA between normal and asthmatic individuals (18). However, since the in situ pattern of the CysLT1 receptor in human lung showed very confined localization to smooth-muscle cells and some macrophages, detailed in situ hybridization and immunohistochemical comparisons of diseased and normal samples will be needed to address pathologic variation in the expression of the CysLT1 receptor. To date, there have been no descriptions of the CysLT1 receptor protein in any tissues or cells. We describe here the distribution of the CysLT1 receptor protein in smooth-muscle cells of normal human lung and in macrophages and peripheral blood leukocytes.
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METHODS |
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
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Production of Antisera and Immunoblot Analyses
Recombinant CysLT1 receptor was expressed in Escherichia coli as a lambda repressor CysLT1 receptor-histidine-tagged fusion protein (R. Breyer, Vanderbilt University, U.S. Patent #09/293179), and the purified antigen was used to raise the goat anti-CysLT1 receptor-specific antisera described in these studies. A goat was injected with 0.5 mg of purified recombinant CysLT1 receptor fusion protein in complete Freund's adjuvant, and received three subsequent booster doses of 0.25 mg of the same antigen in incomplete Freund's adjuvant. The antiserum so obtained was titrated with an enzyme-linked immunosorbent assay (ELISA) against the purified recombinant CysLT1 receptor, and was shown to specifically recognize the receptor protein at a dilution of ~ 1:1,000. For immunoblot analyses, purified recombinant CysLT1 receptor (8 to 160 ng) was separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis on 4%- to 20%-gradient Novex gels and blotted onto nitrocellulose overnight at 100 mA. Blots were blocked for 1 h in 5% milk in Tris-buffered saline containing 0.1% Tween (pH 7.4) (TTBS). Blots were incubated with immune antisera at a 1:5,000 dilution in 5% milk/TTBS for 60 min, washed three times in TTBS, and incubated with a sheep anti goat second antibody (Amersham, Arlington Heights, IL) at a 1:3,000 dilution in 3% milk/TTBS for 60 min. Blots were washed three times in Tris-buffered saline containing 0.3% Tween-20, rinsed with TBS, and subjected to enhanced chemiluminescence (ECL) detection (Amersham) according to the manufacturer's instructions.
Purification of Peripheral Blood Mononuclear Cells Minus T Cells, T Cells, and Eosinophils
Standard blood-cell purification methods were used to obtain partially purified preparations from normal donors. Peripheral blood mononuclear cells (PBMC) were isolated from buffy-coat preparations by centrifugation over lymphocyte separation medium (LSM) (ICN). T cells were rosetted by the incubation of PBMC with neuraminidase-treated sheep red blood cells (SRBC) and were pelleted through LSM. The SRBC were removed by lysis with ammonium chloride potassium (ACK) lysis buffer (Gibco/BRL, Rockville, MD). T-cell-depleted PBMC accumulated at the interface of the LSM. Eosinophils were prepared from peripheral blood from donors known to have increased peripheral blood eosinophils counts as a result of seasonal allergies, but with no history of asthmatic disease. Erythrocytes were removed by hypotonic lysis of the pelleted cells, followed by negative selection with anti-CD16 microbeads (Miltenyi Biotech, Auburn, CA) according to the manufacturer's instructions. Purity of eosinophil preparations was > 90%, with some contaminating monocytes and neutrophils. All preparations of cells were either pelleted in 4% paraformaldehyde or were fresh frozen before in situ hybridization or immunohistochemistry.
In Situ Hybridization and Immunohistochemistry
Purified blood cells were prepared for histologic analysis by one of the
following two methods: (1) fresh-frozen cells were rinsed in phosphate-buffered saline (PBS) (Sigma, St. Louis, MO), made with diethylpyrocarbonate-treated water (Eppendorf, Westbury, NY), resuspended in
ornithine carbamyltransferase (OCT) solution (Miles, Elkhart, IN), and
frozen at 
20° C; or (2) cells were pelleted and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA), dehydrated in graded ethanols, and processed to paraffin. Frozen normal
lung biopsy samples (National Disease Research Interchange, Philadelphia, PA) or frozen purified cells were embedded in OCT solution, sectioned at 8 µm, thaw-mounted onto Superfrost Plus slides (Fisher Scientific, Pittsburgh, PA) and fixed for 30 min in 4% paraformaldehyde.
Cells embedded in paraffin were dewaxed, rehydrated, and digested for
10 min in 10 µg/ml proteinase K (Boehringer Mannheim, Indianapolis,
IN) at room temperature. Oligonucleotide antisense and sense CysLT1 receptor mRNA probes were prepared, and in situ hybridization was
done as previously described (17). Bound probe was detected with the
TSA direct red fluorescence in situ hybridization tyramide amplification kit (NEN Life Sciences, Boston, MA) according to the manufacturer's instructions. Double-label cell identification immunohistochemistry was performed on specimens that showed a fluorescence signal for
CysLT1 receptor mRNA. Cells were rinsed in PBS, blocked with 10%
normal donkey serum (Jackson Immunoresearch, West Grove, PA),
and incubated for 2 h with the appropriate cell-surface identification
marker according to the manufacturer's recommended procedures.
Identification marker antisera used on PBMC preparations were CD19
(Pharmingen, San Diego, CA), a marker for B-precursor and B cells;
CD18 (Pharmingen), a marker for monocytes and granulocytes; CD34
(Pharmingen), a marker for pluripotent hematopoietic stem cells; LN5
(Zymed, San Francisco, CA), a macrophage marker; CD14 (Serotec,
Oxford, UK), a marker for monocytes, granulocytes, and dendritic
cells; and an antiserum to the IL-5 receptor 
-subunit (R&D Systems,
Inc., Minneapolis, MN).
Identification-marker antisera used on T cells were CD4 (Dako, Carpinteria, CA) and CD8 (Dako). The identification marker for eosinophils was the CCR3 receptor, detected with an antiserum from Bruce Daugherty (Merck Research Labs) (21). In the last-named case, immunohistochemistry was done on saponin-treated cells with extensive blocking. Immune-specific signal was detected with the appropriate fluorescein isothiocyanate-labeled donkey secondary antisera (Jackson Immunoresearch). Immune-complexed sections were rinsed and counterstained with DAPI (Molecular Probes, Eugene, OR). Images were acquired, processed, and quantified with a Nikon E1000 microscope (Nikon, Tokyo, Japan), Micromax CCD camera (Princeton Instruments, San Diego, CA), and Metamorph imaging program (Universal Imaging, West Chester, PA).
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RESULTS |
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
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In order to investigate the distribution of CysLT1 receptor mRNA and protein in human lung and peripheral blood cells, we made a specific oligonucleotide probe, 5'-GAACATAATAGACCACACGGAGAGGCAGTG-3' (antisense) and antiserum (17). The oligonucleotide antisense probe had previously been shown to recognize CysLT1 receptor mRNA in human lung muscle cells and macrophages (17). The antiserum, first described in the present study, showed specific recognition of the purified CysLT1 receptor on immunoblot analysis (Figure 1A). We were unable to detect the receptor in immunoblot analyses of lung tissue or COS cells transfected with the CysLT1 receptor membrane fractions (data not shown). We calculated from the expression levels of the CysLT1 receptor in human lung or in COS CysLT1 receptor-transfected membranes (both having about 50 fmol/mg protein), and from the sensitivity of the antiserum to recombinant receptor protein, that unless the receptor was concentrated in some fashion, its concentration would be below the detection limits of Western blot analysis. The monomeric recombinant CysLT1 receptor had a molecular weight of approximately 42 kD, but much larger amounts of dimerized and oligomerized receptors were observed in these preparations even in the presence of the denaturing gel detergents (Figure 1A). No CysLT1 receptor signal was seen with antiserum adsorbed with 10 µg/ml purified CysLT1 recombinant receptor or with control, nonimmune goat antisera (Figures 1B and 1C).
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Since immunohistochemistry is more sensitive than immunoblot analysis, we conducted immunostaining on COS cells transiently transfected with the CysLT1 receptor and with COS cells alone, and compared the results (Figures 1D through 1G). COS cells alone showed no signal with the antisera (Figure 1D), in contrast to the positive staining observed in CysLT1 receptor-transfected COS cells (Figure 1E). No signal was seen in the same sample with normal goat serum (Figure 1G), and the positive staining for CysLT1 receptor on COS CysLT1-transfected cells was eliminated by preadsorption with recombinant CysLT1 receptor protein (Figure 1F).
In normal human lung, CysLT1 receptor mRNA was expressed in smooth-muscle fibers (Figures 2A and 2B), in accord with our previously reported findings (17). Using the specific CysLT1 antiserum, we found that CysLT1 receptor protein coincided with expression of the receptor mRNA (Figure 2C). The specificity of the immunohistochemical identification of the CysLT1 receptor protein was shown by the absence of signal after preincubation of the antiserum with recombinant CysLT1 protein (Figure 2D). In addition, CysLT1 receptor mRNA and protein were identified in human lung interstitial macrophages by colocalization with LN5 panmacrophage antigen (Figures 2E through 2I). At higher magnification, the CysLT1 receptor protein signal appeared to be punctated (Figures 2H and 2I). The intimate spatial relationship of a lung macrophage and a smooth-muscle cell, both expressing the CysLT1 receptor, is shown in Figures 2H and 2I.
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Because CysLTs were observed in lung macrophages, and
have been suggested to contribute significantly to the inflammatory components of asthma (22), we investigated the expression of the CysLT1 receptor in human peripheral blood
cells. In PBMC from which T cells had been removed, we observed CysLT1 receptor mRNA and protein in about 20% of
the total cell population (Figures 3A through 3D; Table 1).
Subsets of CysLT1-positive PBMC coexpressed CD14 (Figures 4A through 4C) and CD19 (Figures 4D through 4F). These antigenic markers have been used previously to identify monocytic and B-lymphocytic cells, respectively (23, 24). In the
PBMC population, immunoreactive labeling of the CysLT1 receptor appeared in clusters (Figures 3C and 4A). The punctate appearance of the CysLT1 receptor protein in peripheral
blood cells may reflect oligomerization of the receptor and/or
membrane compartmentalization with other proteins or lipids,
as has been shown for the chemotactic formyl-met-leu-phe
receptor (25). In the PBMC preparation, all of the rarely found
pregranulocytic CD34+-staining cells (< 1 per 1,000 cells) (25)
coexpressed the CysLT1 receptor (Figures 4G through 4I).
The CysLT1 receptor was also found to be coexpressed with
some, but not all, cells expressing the IL-5 receptor -subunit
(Figures 4J through 4L).
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Because eosinophils have been suggested to exacerbate inflammation in asthma, and CysLTs have been shown to be potent chemoattractants for eosinophils both in vitro and in vivo (27), we next investigated whether the CysLT1 receptor is expressed on human peripheral blood eosinophils. In purified populations of eosinophils from hypereosinophilic but nonasthmatic subjects, we observed specific expression of CysLT1 receptor mRNA and protein (Figures 5A and 5D). The majority of these cells also showed immunoreactivity for the CCR3 receptor, a beta-chemokine receptor expressed on eosinophils (21) (Figures 5D through 5F; Table 1).
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In a population of T cells purified from our samples of peripheral blood cells from nonasthmatic subjects, the CysLT1 receptor mRNA or protein was rarely present, in contrast to the abundant CysLT1 receptor expression demonstrated on eosinophils and some classes of monocytes (CD14+) and B lymphocytes (CD19+) (Figures 6A through 6D; Table 1). We were able to demonstrate both CD4+ and CD8+ helper T cells in this preparation, and neither of these subsets of T cells expressed the CysLT1 receptor (Figures 6A through 6D). CysLT1 receptor expression may be upregulated in lymphocytes from asthmatic subjects. Quantification of the expression of CysLT1 receptor mRNA and protein in populations of normal human peripheral blood cells is shown in Table 1.
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DISCUSSION |
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ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
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We report here the immunohistochemical identification of the CysLT1 receptor protein in normal human lung and normal human peripheral blood cells, in accord with the in situ expression of CysLT1 mRNA in human lung smooth-muscle cells and macrophages demonstrated by the molecular cloning of the CysLT1 receptor (17). With a panel of peripheral blood cell markers, we further found that the receptor is expressed in cells of particular relevance to asthma and atopy, namely eosinophils, monocytes/macrophages, and B lymphocytes, and in CD34+ granulocytic precursor cells. The demonstration of the CysLT1 receptor on eosinophils concurs with observations that CysLT1 receptor antagonists inhibit eosinophil chemotaxis and airway inflammation (30, 31), and that CysLTs partly mediate eotaxin-induced bronchial hyperresponsiveness and eosinophilia in IL-5-transgenic mice (32). The expression of the CysLT1 receptor on monocytes is consistent with previous CysLT1 receptor binding characterizations and with functional activation studies of premyelocytic HL-60 and THP-1 cells (9). Ours is the first demonstration of the CysLT1 receptor on B lymphocytes, although the receptor has been well characterized on the lymphoblastoid U937 cell line, and B lymphocytes have been shown to produce leukotrienes under some conditions (9, 33). The demonstration of the CysLT1 receptor on CD34+ pregranulocytic cells is intriguing, given the detection of increased numbers of CD34+ cells in bronchial biopsy specimens from atopic asthmatic and atopic nonasthmatic subjects (34). The receptor was neither in peripheral polymorphonuclear leukocytes nor in the majority of T cells, in accord with most reports in the literature (9).
The expression of the CysLT receptor on eosinophils and
monocytes, both of which are cell types capable of synthesizing
CysLTs, suggests that both autocrine and paracrine activation
may occur after stimulation by the appropriate inflammatory
signal. The expression of the CysLT1 receptor on pregranulocytic CD34+ cells raises the possibility that under some conditions, CysLTs may influence the differentiation pathway of
these cells. A subset of cells expressing the IL-5 receptor -chain (common to the IL-5, IL-3, and granulocyte-macrophage
colony-stimulating factor receptors) also coexpressed the
CysLT1 receptor. We hypothesize that CysLTs prime the maturation of CD34+ precursor cells to eosinophils, monocytes/
macrophages, and subsets of lymphocytic cells in the peripheral blood compartment (Figure 7). During an asthmatic exacerbation, CysLTs produced in the lung by eosinophils and macrophages (and also by mast cells in an atopic patient) may
activate bronchial smooth-muscle CysLT1 receptors, producing
bronchoconstriction. In addition, the CysLTs may act in an
autocrine and paracrine fashion on CysLT1 receptors on interstitial precursor granulocytes, eosinophils, and macrophages,
thereby amplifying both the immediate lung damage and expanding the populations of inflammatory cells in the lung
(Figure 7). Such a positive feedback mechanism may in part
explain the clinically observed progressively refractory bronchospasm and inflammation that characterize asthma.
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Our characterization of the expression of the CysLT1 receptor in the lung is consistent with that in many pharmacologic and clinical studies of the effects of CysLTs and specific CysLT1 receptor antagonists. In addition, the localization of the CysLT1 receptor on subsets of B-lymphocytic (CD19+) and pregranulocytic (CD34+) cells suggests undiscovered roles of CysLTs in these cell types. Future work will focus on the molecular expression and activation of the receptor in asthma and other diseases.
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Footnotes |
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Correspondence and requests for reprints should be addressed to David J. Figueroa, Department of Pharmacology, Merck Research Laboratories, Sumneytown Pike, West Point, PA 19486.
(Received in original form March 16, 2000 and in revised form June 29, 2000).
Acknowledgments: The authors thank Drs. H. Zweerink, J. Mudgett, M. Cascieri, F. Nantel, A. W. Ford-Hutchinson, C. T. Caskey, and T. Reiss for support of this project.
Supported in part by NIH Grants DK46205 and GM-15431 (R.M.B).
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INTRODUCTION
METHODS
RESULTS
DISCUSSION
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 M. Profita, A. Sala, A. Bonanno, L. Siena, M. Ferraro, R. Di Giorgi, A. M. Montalbano, G. D. Albano, R. Gagliardo, and M. Gjomarkaj Cysteinyl Leukotriene-1 Receptor Activation in a Human Bronchial Epithelial Cell Line Leads to Signal Transducer and Activator of Transcription 1-Mediated Eosinophil Adhesion J. Pharmacol. Exp. Ther., June 1, 2008; 325(3): 1024 - 1030. [Abstract] [Full Text] [PDF]
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 W. Jiang, S. R. Hall, M. P.W. Moos, R. Y. Cao, S. Ishii, K. O. Ogunyankin, L. G. Melo, and C. D. Funk Endothelial Cysteinyl Leukotriene 2 Receptor Expression Mediates Myocardial Ischemia-Reperfusion Injury Am. J. Pathol., March 1, 2008; 172(3): 592 - 602. [Abstract] [Full Text] [PDF]
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 A. K. Mehta, S. N. Gaur, N. Arora, and B. P. Singh Effect of choline chloride in allergen-induced mouse model of airway inflammation Eur. Respir. J., October 1, 2007; 30(4): 662 - 671. [Abstract] [Full Text] [PDF]
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 D. A. Deshpande, R. M. Pascual, S.-W. Wang, D. M. Eckman, E. C. Riemer, C. D. Funk, and R. B. Penn PKC-dependent regulation of the receptor locus dominates functional consequences of cysteinyl leukotriene type 1 receptor activation FASEB J, August 1, 2007; 21(10): 2335 - 2342. [Abstract] [Full Text] [PDF]
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C. Thompson, A. Cloutier, Y. Bosse, M. Thivierge, C. L. Gouill, P. Larivee, P. P. McDonald, J. Stankova, and M. Rola-Pleszczynski CysLT1 Receptor Engagement Induces Activator Protein-1- and NF-{kappa}B-Dependent IL-8 Expression Am. J. Respir. Cell Mol. Biol., December 1, 2006; 35(6): 697 - 704. [Abstract] [Full Text] [PDF]
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D. C. Kim, F. I. Hsu, N. A. Barrett, D. S. Friend, R. Grenningloh, I-C. Ho, A. Al-Garawi, J. M. Lora, B. K. Lam, K. F. Austen, et al. Cysteinyl Leukotrienes Regulate Th2 Cell-Dependent Pulmonary Inflammation J. Immunol., April 1, 2006; 176(7): 4440 - 4448. [Abstract] [Full Text] [PDF]
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 W. R. Henderson Jr., G. K. S. Chiang, Y.-t. Tien, and E. Y. Chi Reversal of Allergen-induced Airway Remodeling by CysLT1 Receptor Blockade Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 718 - 728. [Abstract] [Full Text] [PDF]
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 J. Zhang, M. A. Alston, H. Huang, and R. L. Rabin Human T cell cytokine responses are dependent on multidrug resistance protein-1 Int. Immunol., March 1, 2006; 18(3): 485 - 493. [Abstract] [Full Text] [PDF]
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J. J. Lima, S. Zhang, A. Grant, L. Shao, K. G. Tantisira, H. Allayee, J. Wang, J. Sylvester, J. Holbrook, R. Wise, et al. Influence of Leukotriene Pathway Polymorphisms on Response to Montelukast in Asthma Am. J. Respir. Crit. Care Med., February 15, 2006; 173(4): 379 - 385. [Abstract] [Full Text] [PDF]
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 V. Capra, S. Ravasi, M. R. Accomazzo, S. Citro, M. Grimoldi, M. P. Abbracchio, and G. E. Rovati CysLT1 receptor is a target for extracellular nucleotide-induced heterologous desensitization: a possible feedback mechanism in inflammation J. Cell Sci., December 1, 2005; 118(23): 5625 - 5636. [Abstract] [Full Text] [PDF]
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J. Zhu, Y.-S. Qiu, D. J. Figueroa, V. Bandi, H. Galczenski, K. Hamada, K. K. Guntupalli, J. F. Evans, and P. K. Jeffery Localization and Upregulation of Cysteinyl Leukotriene-1 Receptor in Asthmatic Bronchial Mucosa Am. J. Respir. Cell Mol. Biol., December 1, 2005; 33(6): 531 - 540. [Abstract] [Full Text] [PDF]
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 M. Fujii, H. Tanaka, and S. Abe Interferon-{gamma} Up-regulates Expression of Cysteinyl Leukotriene Type 2 Receptors on Eosinophils in Asthmatic Patients Chest, November 1, 2005; 128(5): 3148 - 3155. [Abstract] [Full Text] [PDF]
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W. Busse and M. Kraft Cysteinyl Leukotrienes in Allergic Inflammation: Strategic Target for Therapy Chest, April 1, 2005; 127(4): 1312 - 1326. [Abstract] [Full Text] [PDF]
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 S. Naik, C. K. Billington, R. M. Pascual, D. A. Deshpande, F. P. Stefano, T. A. Kohout, D. M. Eckman, J. L. Benovic, and R. B. Penn Regulation of Cysteinyl Leukotriene Type 1 Receptor Internalization and Signaling J. Biol. Chem., March 11, 2005; 280(10): 8722 - 8732. [Abstract] [Full Text] [PDF]
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 P. J. Barnes Distribution of Receptor Targets in the Lung Proceedings of the ATS, December 1, 2004; 1(4): 345 - 351. [Abstract] [Full Text] [PDF]
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K. Okunishi, M. Dohi, K. Nakagome, R. Tanaka, and K. Yamamoto A Novel Role of Cysteinyl Leukotrienes to Promote Dendritic Cell Activation in the Antigen-Induced Immune Responses in the Lung J. Immunol., November 15, 2004; 173(10): 6393 - 6402. [Abstract] [Full Text] [PDF]
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T. C. Beller, A. Maekawa, D. S. Friend, K. F. Austen, and Y. Kanaoka Targeted Gene Disruption Reveals the Role of the Cysteinyl Leukotriene 2 Receptor in Increased Vascular Permeability and in Bleomycin-induced Pulmonary Fibrosis in Mice J. Biol. Chem., October 29, 2004; 279(44): 46129 - 46134. [Abstract] [Full Text] [PDF]
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Y. Kanaoka and J. A. Boyce Cysteinyl Leukotrienes and Their Receptors: Cellular Distribution and Function in Immune and Inflammatory Responses J. Immunol., August 1, 2004; 173(3): 1503 - 1510. [Abstract] [Full Text] [PDF]
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A. D. Goldbart, J. L. Goldman, R. C. Li, K. R. Brittian, R. Tauman, and D. Gozal Differential Expression of Cysteinyl Leukotriene Receptors 1 and 2 in Tonsils of Children With Obstructive Sleep Apnea Syndrome or Recurrent Infection Chest, July 1, 2004; 126(1): 13 - 18. [Abstract] [Full Text] [PDF]
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 G. Yang, A. Haczku, H. Chen, V. Martin, H. Galczenski, Y. Tomer, C. R. Van Beisen, J. F. Evans, R. A. Panettieri, and C. D. Funk Transgenic smooth muscle expression of the human CysLT1 receptor induces enhanced responsiveness of murine airways to leukotriene D4 Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L992 - L1001. [Abstract] [Full Text] [PDF]
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K. Parameswaran, R. Watson, G. M. Gauvreau, R. Sehmi, and P. M. O'Byrne The Effect of Pranlukast on Allergen-induced Bone Marrow Eosinophilopoiesis in Subjects with Asthma Am. J. Respir. Crit. Care Med., April 15, 2004; 169(8): 915 - 920. [Abstract] [Full Text] [PDF]
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 T. C. Beller, D. S. Friend, A. Maekawa, B. K. Lam, K. F. Austen, and Y. Kanaoka Cysteinyl leukotriene 1 receptor controls the severity of chronic pulmonary inflammation and fibrosis PNAS, March 2, 2004; 101(9): 3047 - 3052. [Abstract] [Full Text] [PDF]
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D. E. Sloniewsky, K. M. Ridge, Y. Adir, F. P. Fries, A. Briva, J. I. Sznajder, and P. H. S. Sporn Leukotriene D4 Activates Alveolar Epithelial Na,K-ATPase and Increases Alveolar Fluid Clearance Am. J. Respir. Crit. Care Med., February 1, 2004; 169(3): 407 - 412. [Abstract] [Full Text] [PDF]
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E. A. Mellor, N. Frank, D. Soler, M. R. Hodge, J. M. Lora, K. F. Austen, and J. A. Boyce Expression of the type 2 receptor for cysteinyl leukotrienes (CysLT2R) by human mast cells: Functional distinction from CysLT1R PNAS, September 30, 2003; 100(20): 11589 - 11593. [Abstract] [Full Text] [PDF]
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T. Nagase, N. Uozumi, T. Aoki-Nagase, K. Terawaki, S. Ishii, T. Tomita, H. Yamamoto, K. Hashizume, Y. Ouchi, and T. Shimizu A potent inhibitor of cytosolic phospholipase A2, arachidonyl trifluoromethyl ketone, attenuates LPS-induced lung injury in mice Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L720 - L726. [Abstract] [Full Text] [PDF]
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K. Chibana, Y. Ishii, T. Asakura, and T. Fukuda Up-Regulation of Cysteinyl Leukotriene 1 Receptor by IL-13 Enables Human Lung Fibroblasts to Respond to Leukotriene C4 and Produce Eotaxin J. Immunol., April 15, 2003; 170(8): 4290 - 4295. [Abstract] [Full Text] [PDF]
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 C. Brink, S.-E. Dahlen, J. Drazen, J. F. Evans, D. W. P. Hay, S. Nicosia, C. N. Serhan, T. Shimizu, and T. Yokomizo International Union of Pharmacology XXXVII. Nomenclature for Leukotriene and Lipoxin Receptors Pharmacol. Rev., March 1, 2003; 55(1): 195 - 227. [Abstract] [Full Text] [PDF]
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K. J. Serio, S. C. Johns, L. Luo, C. R. Hodulik, and T. D. Bigby Lipopolysaccharide Down-Regulates the Leukotriene C4 Synthase Gene in the Monocyte-Like Cell Line, THP-1 J. Immunol., February 15, 2003; 170(4): 2121 - 2128. [Abstract] [Full Text] [PDF]
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W. M. Abraham Of Mice and Men Am. J. Respir. Cell Mol. Biol., January 1, 2003; 28(1): 1 - 4. [Full Text] [PDF]
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T. Aoki-Nagase, T. Nagase, Y. Oh-hashi, T. Shindo, Y. Kurihara, Y. Yamaguchi, H. Yamamoto, T. Tomita, E. Ohga, R. Nagai, et al. Attenuation of antigen-induced airway hyperresponsiveness in CGRP-deficient mice Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L963 - L970. [Abstract] [Full Text] [PDF]
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 C. Bandeira-Melo, L. J. Woods, M. Phoofolo, and P. F. Weller Intracrine Cysteinyl Leukotriene Receptor-mediated Signaling of Eosinophil Vesicular Transport-mediated Interleukin-4 Secretion J. Exp. Med., September 16, 2002; 196(6): 841 - 850. [Abstract] [Full Text] [PDF]
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A. Maekawa, K. F. Austen, and Y. Kanaoka Targeted Gene Disruption Reveals the Role of Cysteinyl Leukotriene 1 Receptor in the Enhanced Vascular Permeability of Mice Undergoing Acute Inflammatory Responses J. Biol. Chem., May 31, 2002; 277(23): 20820 - 20824. [Abstract] [Full Text] [PDF]
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E. A. Mellor, K. F. Austen, and J. A. Boyce Cysteinyl Leukotrienes and Uridine Diphosphate Induce Cytokine Generation by Human Mast Cells Through an Interleukin 4-regulated Pathway that Is Inhibited by Leukotriene Receptor Antagonists J. Exp. Med., March 4, 2002; 195(5): 583 - 592. [Abstract] [Full Text] [PDF]
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M. J. TOBIN Asthma, Airway Biology, and Nasal Disorders in AJRCCM 2001 Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 598 - 618. [Full Text] [PDF]
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W. R. HENDERSON JR., L.-O. TANG, S.-J. CHU, S.-M. TSAO, G. K. S. CHIANG, F. JONES, M. JONAS, C. PAE, H. WANG, and E. Y. CHI A Role for Cysteinyl Leukotrienes in Airway Remodeling in a Mouse Asthma Model Am. J. Respir. Crit. Care Med., January 1, 2002; 165(1): 108 - 116. [Abstract] [Full Text] [PDF]
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Y. AMRANI, P. E. MOORE, R. HOFFMAN, S. A. SHORE, and R. A. PANETTIERI JR. Interferon-gamma Modulates Cysteinyl Leukotriene Receptor-1 Expression and Function in Human Airway Myocytes Am. J. Respir. Crit. Care Med., December 1, 2001; 164(11): 2098 - 2101. [Abstract] [Full Text] [PDF]
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 F. Bautz, C. Denzlinger, L. Kanz, and R. Mohle Chemotaxis and transendothelial migration of CD34+ hematopoietic progenitor cells induced by the inflammatory mediator leukotriene D4 are mediated by the 7-transmembrane receptor CysLT1 Blood, June 1, 2001; 97(11): 3433 - 3440. [Abstract] [Full Text] [PDF]
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