This Article Full Text Full Text (PDF) All Versions of this Article: 200912-1845OCv1 182/1/92    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Kheirandish-Gozal, L. Articles by Gozal, D. PubMed PubMed Citation Articles by Kheirandish-Gozal, L. Articles by Gozal, D.

Endothelial Progenitor Cells and Vascular Dysfunction in Children with Obstructive Sleep Apnea

¹ Section of Pediatric Sleep Medicine, Department of Pediatrics, University of Chicago, Chicago, Illinois; and ² Division of Pediatric Sleep Medicine, Department of Pediatrics, University of Louisville, Louisville, Kentucky

Correspondence and requests for reprints should be addressed to Leila Kheirandish-Gozal, M.D., Section of Pediatric Sleep Medicine, Department of Pediatrics, The University of Chicago, 5721 S. Maryland Avenue, MC 8000, Suite K-160, Chicago, IL 60637. E-mail: lgozal{at}peds.bsd.uchicago.edu

Rationale: Endothelial dysfunction is a potential complication of obstructive sleep apnea syndrome (OSAS) in children ascribed to systemic inflammatory changes. However, not all children with OSAS will manifest endothelial dysfunction.

Objectives: The variability in endothelial function in pediatric OSAS may be related to the ability to recruit repair mechanisms such as endothelial progenitor cells (EPCs).

Methods: Prepubertal nonhypertensive children with or without polysomonographically confirmed OSAS had endothelial function assessed in a morning fasted state using a modified hyperemic test involving cuff-induced occlusion of the radial and ulnar arteries. Blood was drawn and EPCs were assessed by flow cytometry and triple staining using antibodies against CD133, CD34, and vascular endothelial growth factor receptor-2 after isolation of peripheral blood mononuclear cells. SDF-1 levels were measured by ELISA.

Measurements and Main Results: Eighty children with OSAS (mean age 8.2 ± 1.4 yr, mean body mass index [BMI] z-score, 1.43 ± 0.3) and 20 controls (CO) matched for BMI, age, sex, and ethnicity were studied. Significant delays to peak capillary reperfusion after occlusion release (Tmax) occurred in OSAS children, but substantial variability was present. Despite similar OSAS severity, EPC counts, and stromal cell–derived factor-1 (SDF-1) levels were significantly lower among the 20 OSAS children with the longest Tmax, when compared with either the 20 children with normal Tmax values or to CO ( P < 0.01). Furthermore, Tmax was significantly and inversely correlated with EPCs (r², 0.51; P < 0.01), but neither EPCs nor Tmax were associated with apnea-hyponea index (AHI).

Conclusions: Endothelial dysfunction is frequently present in OSAS. Variance in endothelial functional phenotype may not only reside in the individual susceptibility but also in the ability to recruit endothelial repair mechanisms.

Key Words: sleep apnea • endothelial progenitor cells • endothelial function • serum lipids • atherosclerosis

AT A GLANCE COMMENTARY Scientific Knowledge on the Subject Obstructive sleep apnea leads to abnormal endothelial function in some but not all children afflicted with this condition. The reasons underlying such differences are unclear. What This Study Adds to the Field The number of circulating endothelial progenitor cells in children with sleep apnea may be an important determinant of the magnitude of endothelial dysfunction.