Published ahead of print on March 15, 2007, doi:10.1164/rccm.200601-134OC
© 2007 American Thoracic Society doi: 10.1164/rccm.200601-134OC
Two-dimensional Analysis of Elements and Mononuclear Cells in Hard Metal Lung Disease1 Division of Respiratory Medicine, Graduate School of Medical and Dental Sciences, Niigata University; 2 Electron Probe Microanalysis Laboratory, Center of Instrumental Analysis, Niigata University; 3 Department of Internal Medicine, JA Niigata Kouseiren Uonuma Hospital; 4 Division of Dental Biomaterial Science, Graduate School of Medical and Dental Sciences, Niigata University; and 5 Department of General Medicine, Niigata University Medical and Dental Hospital, Niigata, Japan Correspondence and requests for reprints should be addressed to Toshinori Takada, M.D., Ph.D., Division of Respiratory Medicine, Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-dori, Niigata 951-8510, Japan. E-mail: ttakada{at}med.niigata-u.ac.jp Rationale: Hard metal lung disease is caused by exposure to hard metal, a synthetic compound that combines tungsten carbide with cobalt as well as a number of other metals. Interstitial lung disease caused by hard metal is uniquely characterized by giant cell interstitial pneumonia. The pathogenesis of hard metal lung disease is unclear. Objectives: To elucidate the distribution of inhaled hard metal and reactive inflammatory cells in biopsy lung tissue from patients with hard metal lung disease. Methods: Seventeen patients with interstitial lung disease in which tungsten was detected and five control subjects were studied. Detection and mapping of elements were performed with an electron probe microanalyzer equipped with a wavelength dispersive spectrometer. We immunohistochemically stained mononuclear cells, in tissue samples available from five patients, with anti-human CD4, CD8, CD20, CD68, and CD163 antibodies, and compared the distribution of positive cells with hard metal elements. Measurements and Main Results: Thirteen of 17 patients were pathologically diagnosed as having giant cell interstitial pneumonia. Tungsten and cobalt were accumulated in the centrilobular fibrotic lesions, but were never found in the control lungs. CD8+ lymphocytes and CD163+ monocyte-macrophages were distributed predominantly in centrilobular fibrotic lesions around the hard metal elements. CD163+ colocalized with tungsten. Small numbers of CD8+ and CD163+ cells were also immunohistochemically shown in peribronchiolar areas and alveolar walls. Conclusions: Macrophages may phagocytose inhaled tungsten via CD163 and play an important role in forming the fibrotic lesion of hard metal lung disease with cytotoxic T lymphocytes.
Key Words: electron probe microanalysis • hard metal • interstitial lung disease • CD163 antigen • CD8-positive T lymphocytes
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