Published ahead of print on May 18, 2006, doi:10.1164/rccm.200509-1420OC
American Journal of Respiratory and Critical Care Medicine Vol 174. pp. 379-385, (2006)
© 2006 American Thoracic Society
doi: 10.1164/rccm.200509-1420OC
Extracellular Matrix Regulates Enhanced Eotaxin Expression in Asthmatic Airway Smooth Muscle Cells
Vivien Chan,
Janette K. Burgess,
Jonathan C. Ratoff,
Brian J. O'Connor,
Anne Greenough,
Tak H. Lee and
Stuart J. Hirst
King's College London School of Medicine, Medical Research Council and Asthma UK Centre in Allergic Mechanisms of Asthma, and Division of Asthma, Allergy, and Lung Biology, London, United Kingdom; Department of Pharmacology, University of Sydney; and Woolcock Institute of Medical Research, Sydney, Australia
Correspondence and requests for reprints should be addressed to Stuart J. Hirst, Ph.D., King's College London School of Medicine, MRC & Asthma UK Centre in Allergic Mechanisms of Asthma, Thomas Guy House, Guy's Hospital Campus, London SE1 9RT, UK. E-mail: stuart.hirst{at}kcl.ac.uk
Rationale: Altered airway smooth muscle (ASM) function and enrichment of the extracellular matrix (ECM) with fibronectin and collagen are key features of asthma. Previously, we have reported these ECM proteins enhance ASM synthetic function.
Objective: We compared ASM cultured from endobronchial biopsies from subjects with and without asthma to assess if asthmatic cells were hypersecretory and determined whether the underlying mechanism involved autocrine ECM production.
Methods and Measurements: Cells from subjects with and without asthma were cultured on plastic or in plates precoated with ECM proteins. Cytokine production was evaluated by enzyme-linked immunosorbent assay and by reverse transcriptasepolymerase chain reaction. Function-blocking integrin antibodies were used to identify integrin involvement.
Results: Baseline eotaxin and its production after stimulation with interleukin (IL)-13, IL-1 , or tumor necrosis factor- was increased (2.5- to 6.0-fold) in ASM cells cultured from subjects with asthma compared with healthy subjects. When seeded on ECM from asthmatic ASM, IL-13dependent eotaxin release from healthy or asthmatic ASM was enhanced compared with culture on healthy ECM. The ECM substrates fibronectin and type I collagen each enhanced IL-13dependent eotaxin release, and Western immunoblot indicated that fibronectin expression was higher in asthmatic ASM cells. Integrin-blocking antibodies revealed that 5 1 was required for more than 50% of the enhanced IL-13dependent eotaxin release by ASM cells from subjects with asthma, whereas 2 1 or v 3 neutralization lacked effect.
Conclusion: The data indicate that ASM cells cultured from subjects with asthma are hypersecretory compared with cells from healthy donors and that autocrine fibronectin secretion acting via 5 1 in part underlies this effect.
Key Words: airway remodeling airway smooth muscle asthma eotaxin extracellular matrix integrin
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