Published ahead of print on March 23, 2006, doi:10.1164/rccm.200509-1485OC
© 2006 American Thoracic Society doi: 10.1164/rccm.200509-1485OC
Intratracheal Recombinant Surfactant Protein D Prevents Endotoxin Shock in the Newborn Preterm LambDivision of Pulmonary Biology, Cincinnati Children's Hospital, University of Cincinnati, Cincinnati, Ohio; and Genzyme Corporation, Framingham, Massachusetts Correspondence and requests for reprints should be addressed to Machiko Ikegami, M.D., Ph.D.,Professor of Pediatrics, Cincinnati Children's Hospital, Division of Pulmonary Biology, 3333 Burnet Avenue, Cincinnati, Ohio 45229-3039. E-mail: machiko.Ikegami{at}cchmc.org Rationale: The susceptibility of neonates to pulmonary and systemic infection has been associated with the immaturity of both lung structure and the immune system. Surfactant protein (SP) D is a member of the collectin family of innate immune molecules that plays an important role in innate host defense of the lung. Objectives: We tested whether treatment with recombinant human SP-D influenced the response of the lung and systemic circulation to intratracheally administered Escherichia coli lipopolysaccharides. Methods: After intratracheal lipopolysaccharide instillation, preterm newborn lambs were treated with surfactant and ventilated for 5 h. Measurement: Survival rate, physiologic lung function, lung and systemic inflammation, and endotoxin level in plasma were evaluated.
Main Results: In control lambs, intratracheal lipopolysaccharides caused septic shock and death associated with increased endotoxin in plasma. In contrast, all lambs treated with recombinant human SP-D were physiologically stable and survived. Leakage of lipopolysaccharides from the lungs to the systemic circulation was prevented by intratracheal recombinant human SP-D. Recombinant human SP-D prevented systemic inflammation and decreased the expression of IL-1 Conclusions: Intratracheal recombinant human SP-D prevented shock caused by endotoxin released from the lung during ventilation in the premature newborn.
Key Words: cytokines • lung compliance • pulmonary surfactant • respiratory distress syndrome • sepsis This article has been cited by other articles:
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, IL-8, and IL-6 in the spleen and liver. Likewise, recombinant human SP-D decreased IL-1
