help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on June 9, 2005, doi:10.1164/rccm.200411-1523OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Supplement
Right arrow All Versions of this Article:
200411-1523OCv1
172/5/597    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Masri, F. A.
Right arrow Articles by Aulak, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Masri, F. A.
Right arrow Articles by Aulak, K. S.
American Journal of Respiratory and Critical Care Medicine Vol 172. pp. 597-605, (2005)
© 2005 American Thoracic Society
doi: 10.1164/rccm.200411-1523OC

Original Article

Abnormalities in Nitric Oxide and Its Derivatives in Lung Cancer

Fares A. Masri, Suzy A. A. Comhair, Thomas Koeck, Weiling Xu, Allison Janocha, Sudakshina Ghosh, Raed A. Dweik, Joseph Golish, Michael Kinter, Dennis J. Stuehr, Serpil C. Erzurum and Kulwant S. Aulak

Departments of Pathobiology, Pulmonary and Critical Care Medicine, and Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation; and Cleveland State University, Cleveland, Ohio

Correspondence and requests for reprints should be addressed to Kulwant S. Aulak, Ph.D., Cleveland Clinic Foundation, 9500 Euclid Avenue/NB30, Cleveland, OH 44195. E-mail: aulakk{at}ccf.org

Rationale: A cellular prooxidant state promotes cells to neoplastic growth, in part because of modification of proteins and their functions. Reactive nitrogen species formed from nitric oxide (NO) or its metabolites, can lead to protein tyrosine nitration, which is elevated in lung cancer. Objective: To determine the alteration in these NO derivatives and the role they may play in contributing to lung carcinogenesis. Methods: We analyzed levels of NO, nitrite (NO2–), nitrate (NO3–), and the location of the protein nitration and identified the proteins that are modified. Measurements and Main Results: Although exhaled NO and NO2– were increased, endothelial NO synthase or inducible NO synthase expression was similar in the tumor and tumor-free regions. However, immunohistochemistry showed that nitrotyrosine was increased in the tumor relative to non–tumor-bearing sections. We used proteomics to identify the modified proteins (two-dimensional polyacrylamide gel electrophoresis; mass spectrometry). Both the degree of nitration and the protein nitration profile were altered. We identified more than 25 nitrated proteins, including metabolic enzymes, structural proteins, and proteins involved in prevention of oxidative damage. Alterations of the biology of NO metabolites and nitration of proteins may contribute to the mutagenic processes and promote carcinogenesis. Conclusions: This study provides evidence in favor of a role for reactive nitrogen and oxygen species in lung cancer.

Key Words: lung cancer • nitric oxide • nitrotyrosine • protein nitration • proteomics




This article has been cited by other articles:


Home page
 Eur Respir JHome page
I. Horvath, Z. Lazar, N. Gyulai, M. Kollai, and G. Losonczy
Exhaled biomarkers in lung cancer
Eur. Respir. J., July 1, 2009; 34(1): 261 - 275.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. E. Nethery, S. Ghosh, S. C. Erzurum, and J. A. Kern
Inactivation of neuregulin-1 by nitration
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L287 - L293.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Y. Terasaki, T. Akuta, M. Terasaki, T. Sawa, T. Mori, T. Okamoto, M. Ozaki, M. Takeya, and T. Akaike
Guanine Nitration in Idiopathic Pulmonary Fibrosis and Its Implication for Carcinogenesis
Am. J. Respir. Crit. Care Med., September 15, 2006; 174(6): 665 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
R. Mehra, B. A. Moore, K. Crothers, J. Tetrault, and D. A. Fiellin
The association between marijuana smoking and lung cancer: a systematic review.
Arch Intern Med, July 10, 2006; 166(13): 1359 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Peikert, U. Specks, C. Farver, S. C. Erzurum, and S. A.A. Comhair
Melanoma Antigen A4 Is Expressed in Non-Small Cell Lung Cancers and Promotes Apoptosis.
Cancer Res., May 1, 2006; 66(9): 4693 - 4700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. R. Jett and Y. E. Miller
Update in lung cancer 2005.
Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 695 - 697.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
I. Dalle-Donne, R. Rossi, R. Colombo, D. Giustarini, and A. Milzani
Biomarkers of Oxidative Damage in Human Disease
Clin. Chem., April 1, 2006; 52(4): 601 - 623.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2005 American Thoracic Society