Published ahead of print on September 22, 2005, doi:10.1164/rccm.200502-274OC
© 2005 American Thoracic Society doi: 10.1164/rccm.200502-274OC
Proteomic Patterns of Preinvasive Bronchial LesionsDivision of Allergy, Pulmonary, and Critical Care Medicine, Department of Medicine; Departments of Biostatistics and Pathology; Mass Spectrometry Research Center; Division of Hematology and Oncology, Departments of Surgery and Cancer Biology, Vanderbilt-Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine; Veterans Affairs Medical Center, Nashville, Tennessee; and Department of Medicine and Pathology, University of Colorado Health Science Center, Denver, Colorado Correspondence and requests for reprints should be addressed to Pierre P. Massion, M.D., Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt-Ingram Comprehensive Cancer Center, 2220 Pierce Avenue, 640 Preston Research Building, Nashville, TN 37232-6838. E-mail: pierre.massion{at}vanderbilt.edu Purpose: A proteomics approach is warranted to further elucidate the molecular steps involved in lung tumor development. We asked whether we could classify preinvasive lesions of airway epithelium according to their proteomic profile. Experimental Design: We obtained matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiles from 10-µm sections of fresh-frozen tissue samples: 25 normal lung, 29 normal bronchial epithelium, and 20 preinvasive and 36 invasive lung tumor tissue samples from 53 patients. Proteomic profiles were calibrated, binned, and normalized before analysis. We performed class comparison, class prediction, and supervised hierarchic cluster analysis. We tested a set of discriminatory features obtained in a previously published dataset to classify this independent set of normal, preinvasive, and invasive lung tissues. Results: We found a specific proteomic profile that allows an overall predictive accuracy of over 90% of normal, preinvasive, and invasive lung tissues. The proteomic profiles of these tissues were distinct from each other within a disease continuum. We trained our prediction model in a previously published dataset and tested it in a new blinded test set to reach an overall 74% accuracy in classifying tumors from normal tissues. Conclusions: We found specific patterns of protein expression of the airway epithelium that accurately classify bronchial and alveolar tissue with normal histology from preinvasive bronchial lesions and from invasive lung cancer. Although further study is needed to validate this approach and to identify biomarkers of tumor development, this is a first step toward a new proteomic characterization of the human model of lung cancer tumorigenesis.
Key Words: early detection • mass spectrometry • preneoplasia • profiling This article has been cited by other articles:
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