help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on December 3, 2004, doi:10.1164/rccm.200408-1010OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Supplement
Right arrow All Versions of this Article:
200408-1010OCv1
171/5/426    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wood, L. G.
Right arrow Articles by Gibson, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wood, L. G.
Right arrow Articles by Gibson, P. G.
American Journal of Respiratory and Critical Care Medicine Vol 171. pp. 426-430, (2005)
© 2005 American Thoracic Society
doi: 10.1164/rccm.200408-1010OC

Original Article

Induced Sputum 8-Isoprostane Concentrations in Inflammatory Airway Diseases

Lisa G. Wood, Manohar L. Garg, Jodie L. Simpson, Trevor A. Mori, Kevin D. Croft, Peter A. B. Wark and Peter G. Gibson

Department of Respiratory and Sleep Medicine, Hunter Medical Research Institute, John Hunter Hospital; Discipline of Nutrition and Dietetics, School of Health Sciences, Faculty of Health, University of Newcastle, Newcastle; School of Medicine and Pharmacology, University of Western Australia, Perth, Australia

Correspondence and requests for reprints should be addressed to Professor Peter G. Gibson, M.B.B.S., Department of Respiratory and Sleep Medicine, John Hunter Hospital, Locked Bag 1, Hunter Region Mail Centre, NSW, 2310, Australia. E-mail: peter.gibson{at}hunter.health.nsw.gov.au

Induced sputum 8-iso-prostaglandin F2{alpha} (PGF2{alpha}) concentrations may be a useful marker of oxidative stress in airways disease. This study examines oxidative stress (measured by 8-iso-PGF2{alpha}) in airway disease according to disease type (asthma and bronchiectasis), disease activity (stable and acute asthma), and disease pattern (intermittent, mild, moderate, and severe persistent asthma). We compared subjects with stable asthma (n = 71) and bronchiectasis (n = 23) with healthy control subjects (n = 29). Another group of patients with asthma (n = 39) were assessed during and after acute exacerbation. Induced sputum 8-iso-PGF2{alpha} concentrations were validated and found to be elevated in subjects with stable asthma and bronchiectasis versus control subjects (median [interquartile range] 216 [103–389] and 698 [264–1,613] ng/L vs. 123 [41–290] ng/L, p < 0.001) and increased as clinical asthma pattern worsened (intermittent 115 [42–153], mild persistent 116 [89–229] ng/L, moderate persistent 183 [110–317] ng/L, severe persistent 387 [102–587] ng/L; p = 0.010). Sputum 8-iso-PGF2{alpha} concentrations were elevated during acute asthma and decreased with recovery (458 [227–950] ng/L vs. 214 [148–304] ng/L, p = 0.0002). We conclude that 8-iso-PGF2{alpha} is involved in the pathophysiology of inflammatory airway diseases, being related to disease type, pattern, and activity. Analysis of 8-iso-PGF2{alpha} concentrations in induced sputum provides a useful tool for monitoring oxidative stress and investigating strategies aimed at reducing oxidative stress in airways disease.

Key Words: isoprostanes • lipid peroxidation • oxidative stress




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Ito, C. Herbert, J. S. Siegle, C. Vuppusetty, N. Hansbro, P. S. Thomas, P. S. Foster, P. J. Barnes, and R. K. Kumar
Steroid-Resistant Neutrophilic Inflammation in a Mouse Model of an Acute Exacerbation of Asthma
Am. J. Respir. Cell Mol. Biol., November 1, 2008; 39(5): 543 - 550.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. Fuschillo, A. De Felice, and G. Balzano
Mucosal inflammation in idiopathic bronchiectasis: cellular and molecular mechanisms
Eur. Respir. J., February 1, 2008; 31(2): 396 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
V. L. Kinnula, H. Ilumets, M. Myllarniemi, A. Sovijarvi, and P. Rytila
8-Isoprostane as a marker of oxidative stress in nonsymptomatic cigarette smokers and COPD
Eur. Respir. J., January 1, 2007; 29(1): 51 - 55.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
P A B Wark and P G Gibson
Asthma exacerbations {middle dot} 3: Pathogenesis.
Thorax, October 1, 2006; 61(10): 909 - 915.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. E. Wenzel and R. Covar
Update in asthma 2005.
Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 698 - 706.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. J. Boyton, J. Smith, R. Ward, M. Jones, L. Ozerovitch, R. Wilson, M. Rose, J. Trowsdale, and D. M. Altmann
HLA-C and Killer Cell Immunoglobulin-like Receptor Genes in Idiopathic Bronchiectasis
Am. J. Respir. Crit. Care Med., February 1, 2006; 173(3): 327 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Higashi, N. Higashi, T. Tsuburai, Y. Takeuchi, M. Taniguchi, H. Mita, A. Saito, K. Takatori, K. Arimura, and K. Akiyama
Involvement of eicosanoids and surfactant protein D in extrinsic allergic alveolitis
Eur. Respir. J., December 1, 2005; 26(6): 1069 - 1073.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Coll. Nutr.Home page
L. G. Wood, M. L. Garg, R. J. Blake, S. Garcia-Caraballo, and P. G. Gibson
Airway and Circulating Levels of Carotenoids in Asthma and Healthy Controls
J. Am. Coll. Nutr., December 1, 2005; 24(6): 448 - 455.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2005 American Thoracic Society 		  ATS Coding and Billing Quarterly