help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on May 6, 2004, doi:10.1164/rccm.200301-030OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow OnlineSupplement
Right arrow All Versions of this Article:
200301-030OCv1
170/3/306    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Peebles, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Peebles, R. S., Jr.
American Journal of Respiratory and Critical Care Medicine Vol 170. pp. 306-312, (2004)
© 2004 American Thoracic Society

Original Article

Respiratory Syncytial Virus in Allergic Lung Inflammation Increases Muc5ac and Gob-5

Koichi Hashimoto, Barney S. Graham, Samuel B. Ho, Kenneth B. Adler, Robert D. Collins, Sandra J. Olson, Weisong Zhou, Tatsuo Suzutani, Phillip W. Jones, Kasia Goleniewska, Jamye F. O'Neal and R. Stokes Peebles, Jr.

Vanderbilt University, School of Medicine, Nashville, Tennessee; Viral Pathogenesis Laboratory and Clinical Trial Core, National Institutes of Health, Bethesda, Maryland; University of Minnesota, Veterans Affairs Medical Center, Minneapolis, Minnesota; North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina; and Fukushima Medical University, Fukushima, Japan

Correspondence and requests for reprints should be addressed to R. Stokes Peebles, Jr., M.D., Center for Lung Research, T-1217 MCN, Vanderbilt University Medical Center, Nashville, TN 37232-2650. E-mail: stokes.peebles{at}vanderbilt.edu

Respiratory syncytial virus (RSV) is associated with wheezing and childhood asthma. We previously reported that RSV infection prolongs methacholine-induced airway hyperresponsiveness in ovalbumin (OVA)-sensitized mice. In addition, allergically sensitized RSV-infected (OVA/RSV) mice had more abundant airway epithelial mucus production compared with OVA mice 14 days after infection, whereas there was almost no mucus in mice that were only RSV infected. We hypothesized that this increased mucus was associated with mucosal expression of Muc5ac, a mucus gene expression in airways, and gob-5, a member of the Ca2+-activated chloride channel family. By histochemical analysis, we found that there was significantly increased staining for gob-5 and Muc5ac in the airways of OVA/RSV mice compared with either OVA mice or allergically sensitized mice that were challenged with inactivated RSV, and virtually no detectable staining in the RSV group. These findings were confirmed by Western blot analysis. The increased mucus expression in the OVA/RSV group was associated with increased lung levels of interleukin-17, a factor known to stimulate airway mucin gene expression. The impact of virus infection combined with allergic inflammation on mucus production may partially explain the more severe disease and airway hyperresponsiveness associated with RSV in the setting of atopy.

Key Words: mucin • MUC5ac • gob-5 • respiratory syncytial virus




This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
S. S. Mohapatra and S. Boyapalle
Epidemiologic, Experimental, and Clinical Links between Respiratory Syncytial Virus Infection and Asthma
Clin. Microbiol. Rev., July 1, 2008; 21(3): 495 - 504.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. A. Wu, J. J. Peluso, J. D. Shanley, L. Puddington, and R. S. Thrall
Murine Cytomegalovirus Influences Foxj1 Expression, Ciliogenesis, and Mucus Plugging in Mice with Allergic Airway Disease
Am. J. Pathol., March 1, 2008; 172(3): 714 - 724.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
M. Kraft, K. B. Adler, J. L. Ingram, A. L. Crews, T. P. Atkinson, C. B. Cairns, D. C. Krause, and H. W. Chu
Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma
Eur. Respir. J., January 1, 2008; 31(1): 43 - 46.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. R. Kim, K. S. Lee, S. J. Park, K. H. Min, K. Y. Lee, Y. H. Choe, Y. R. Lee, J. S. Kim, S. J. Hong, and Y. C. Lee
PTEN Down-Regulates IL-17 Expression in a Murine Model of Toluene Diisocyanate-Induced Airway Disease
J. Immunol., November 15, 2007; 179(10): 6820 - 6829.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. D. Rudd, M. A. Schaller, J. J. Smit, S. L. Kunkel, R. Neupane, L. Kelley, A. A. Berlin, and N. W. Lukacs
MyD88-Mediated Instructive Signals in Dendritic Cells Regulate Pulmonary Immune Responses during Respiratory Virus Infection
J. Immunol., May 1, 2007; 178(9): 5820 - 5827.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
A. E. Hegab, T. Sakamoto, A. Nomura, Y. Ishii, Y. Morishima, T. Iizuka, T. Kiwamoto, Y. Matsuno, S. Homma, and K. Sekizawa
Niflumic Acid and AG-1478 Reduce Cigarette Smoke-Induced Mucin Synthesis: The Role of hCLCA1
Chest, April 1, 2007; 131(4): 1149 - 1156.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
L. G. Wood, H. Powell, T. Grissell, T. T.D. Nguyen, D. Shafren, M. Hensley, and P. G. Gibson
Persistent Airway Obstruction After Virus Infection Is Not Associated With Airway Inflammation
Chest, February 1, 2007; 131(2): 415 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. J. Long, J. P. Sypek, R. Askew, S. C. Fish, L. E. Mason, C. M. M. Williams, and S. J. Goldman
Gob-5 Contributes to Goblet Cell Hyperplasia and Modulates Pulmonary Tissue Inflammation
Am. J. Respir. Cell Mol. Biol., September 1, 2006; 35(3): 357 - 365.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
N. W. Lukacs, M. L. Moore, B. D. Rudd, A. A. Berlin, R. D. Collins, S. J. Olson, S. B. Ho, and R. S. Peebles Jr
Differential Immune Responses and Pulmonary Pathophysiology Are Induced by Two Different Strains of Respiratory Syncytial Virus
Am. J. Pathol., September 1, 2006; 169(3): 977 - 986.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
J-W Jeong, K Y Lee, J P Lydon, and F J DeMayo
Steroid hormone regulation of Clca3 expression in the murine uterus.
J. Endocrinol., June 1, 2006; 189(3): 473 - 484.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. M. Casalino-Matsuda, M. E. Monzon, and R. M. Forteza
Epidermal Growth Factor Receptor Activation by Epidermal Growth Factor Mediates Oxidant-Induced Goblet Cell Metaplasia in Human Airway Epithelium
Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 581 - 591.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. L. Miller, C. Gerard, M. Schaller, A. D. Gruber, A. A. Humbles, and N. W. Lukacs
Deletion of CCR1 Attenuates Pathophysiologic Responses during Respiratory Syncytial Virus Infection
J. Immunol., February 15, 2006; 176(4): 2562 - 2567.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
R. S. Peebles Jr. and B. S. Graham
Pathogenesis of Respiratory Syncytial Virus Infection in the Murine Model
Proceedings of the ATS, August 1, 2005; 2(2): 110 - 115.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. Fabbri, S. P. Peters, I. Pavord, S. E. Wenzel, S. C. Lazarus, W. MacNee, F. Lemaire, and E. Abraham
Allergic Rhinitis, Asthma, Airway Biology, and Chronic Obstructive Pulmonary Disease in AJRCCM in 2004
Am. J. Respir. Crit. Care Med., April 1, 2005; 171(7): 686 - 698.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Oda, P. B. Canelos, D. M. Essayan, B. A. Plunkett, A. C. Myers, and S.-K. Huang
Interleukin-17F Induces Pulmonary Neutrophilia and Amplifies Antigen-induced Allergic Response
Am. J. Respir. Crit. Care Med., January 1, 2005; 171(1): 12 - 18.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2004 American Thoracic Society 		  ATS State of the Art Course 2008