help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on January 31, 2003, doi:10.1164/rccm.200207-736BC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Supplement
Right arrow All Versions of this Article:
200207-736BCv1
167/11/1478    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lesprit, P.
Right arrow Articles by Carbon, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lesprit, P.
Right arrow Articles by Carbon, C.
American Journal of Respiratory and Critical Care Medicine Vol 167. pp. 1478-1482, (2003)
© 2003 American Thoracic Society

Brief Communication

Role of the Quorum-sensing System in Experimental Pneumonia due to Pseudomonas aeruginosa in Rats

Philippe Lesprit, François Faurisson, Olivier Join-Lambert, Françoise Roudot-Thoraval, Maryline Foglino, Christiane Vissuzaine and Claude Carbon

INSERM EMI-U 9933, and Service d'Anatomie Pathologique, Hôpital Bichat, Paris, France; Département de Santé Publique, Hôpital Henri Mondor, Créteil; and Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Centre National de la Recherche Scientifique, Marseille, France

Correspondence and requests for reprints should be addressed to Dr. Philippe Lesprit, Service d'Immunologie Clinique, Hôpital Henri Mondor, 51 avenue du Maréchal de Lattre, 94010 Créteil, France. E-mail: phillipe.lesprit{at}hmn.ap-hop-paris.fr

The virulence of Pseudomonas aeruginosa is partly controlled by the las quorum-sensing system. A rat model of acute pneumonia was used to investigate the pathophysiological impact of this system by comparing the virulence of the wild-type virulent laboratory strain PAO1 with that of its lasR-deleted mutant PAOR. In comparison with PAO1, PAOR was avirulent after an instillation of 106 cfu (mortality rates, 72 versus 0%, respectively; p < 0.0001). A ten-fold higher inoculum slightly increased the mortality rate induced by PAOR (25%), which remained lower than that induced by PAO1 (75%, p = 0.0001). In addition, with both inocula lung and bronchoalveolar lavage bacterial counts were significantly lower in rats infected with PAOR than with PAO1 (p <= 0.01). Histopathological analysis showed that PAO1 induced a drastic vascular congestion and neutrophil infiltration of the lungs, whereas lung injury in rats infected with PAOR was mild with predominantly macrophage infiltration. This study adds evidence that the quorum-sensing system has an important role in the pathophysiology of P. aeruginosa pulmonary infection.

Key Words: cell-to-cell signaling • experimental pneumonia • Pseudomonas aeruginosa




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
C. N. Wilder, G. Allada, and M. Schuster
Instantaneous Within-Patient Diversity of Pseudomonas aeruginosa Quorum-Sensing Populations from Cystic Fibrosis Lung Infections
Infect. Immun., December 1, 2009; 77(12): 5631 - 5639.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Kohler, A. Buckling, and C. van Delden
Cooperation and virulence of clinical Pseudomonas aeruginosa populations
PNAS, April 14, 2009; 106(15): 6339 - 6344.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. F. Teiber, S. Horke, D. C. Haines, P. K. Chowdhary, J. Xiao, G. L. Kramer, R. W. Haley, and D. I. Draganov
Dominant Role of Paraoxonases in Inactivation of the Pseudomonas aeruginosa Quorum-Sensing Signal N-(3-Oxododecanoyl)-L-Homoserine Lactone
Infect. Immun., June 1, 2008; 76(6): 2512 - 2519.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
O. Epaulard, M. Derouazi, C. Margerit, R. Marlu, D. Filopon, B. Polack, and B. Toussaint
Optimization of a Type III Secretion System-Based Pseudomonas aeruginosa Live Vector for Antigen Delivery
Clin. Vaccine Immunol., February 1, 2008; 15(2): 308 - 313.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. J. Kazmierczak, M. Wiedmann, and K. J. Boor
Alternative Sigma Factors and Their Roles in Bacterial Virulence
Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 527 - 543.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. T. Sadikot, T. S. Blackwell, J. W. Christman, and A. S. Prince
Pathogen-Host Interactions in Pseudomonas aeruginosa Pneumonia
Am. J. Respir. Crit. Care Med., June 1, 2005; 171(11): 1209 - 1223.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
E. Valade, F. M. Thibault, Y. P. Gauthier, M. Palencia, M. Y. Popoff, and D. R. Vidal
The PmlI-PmlR Quorum-Sensing System in Burkholderia pseudomallei Plays a Key Role in Virulence and Modulates Production of the MprA Protease
J. Bacteriol., April 15, 2004; 186(8): 2288 - 2294.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. Tobin
Tuberculosis, Lung Infections, Interstitial Lung Disease, Social Issues and Journalology in AJRCCM 2003
Am. J. Respir. Crit. Care Med., January 15, 2004; 169(2): 288 - 300.
[Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Cabrol, A. Olliver, G. B. Pier, A. Andremont, and R. Ruimy
Transcription of Quorum-Sensing System Genes in Clinical and Environmental Isolates of Pseudomonas aeruginosa
J. Bacteriol., December 15, 2003; 185(24): 7222 - 7230.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. S. Prince
New Insights from an Old Model
Am. J. Respir. Crit. Care Med., June 1, 2003; 167(11): 1462 - 1463.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2003 American Thoracic Society 		  Red In Translatin