help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burgess, J. K.
Right arrow Articles by Black, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burgess, J. K.
Right arrow Articles by Black, J. L.
American Journal of Respiratory and Critical Care Medicine Vol 167. pp. 71-77, (2003)
© 2003 American Thoracic Society

Original Article

Expression of Connective Tissue Growth Factor in Asthmatic Airway Smooth Muscle Cells

Janette K. Burgess*, Peter R. A. Johnson*, Qi Ge, Wendy W. Au, Maree H. Poniris, Brent E. McParland, Greg King, Michael Roth and Judith L. Black

Department of Pharmacology, University of Sydney; and Woolcock Institute of Medical Research, Royal Prince Alfred Hospital, Sydney, Australia

Correspondence and requests for reprints should be addressed to Dr. Janette Burgess, Ph.D., Respiratory Research Group, Department of Pharmacology, Bosch Building, D05 University of Sydney, Sydney NSW, Australia, 2006. E-mail: janette{at}pharmacol.usyd.edu.au

There is strong evidence to implicate transforming growth factor-ß in the remodeling that occurs in asthma, as levels are increased in bronchial lavage fluid and gene expression is increased in bronchial tissue. Transforming growth factor-ß is also known to increase the release of collagen from airway smooth muscle. Here we identify for the first time a possible mechanism for the effects of transforming growth factor-ß. Transforming growth factor-ß specifically induces mRNA and protein for connective tissue growth factor in airway smooth muscle, and moreover, we report that the connective tissue growth factor response is greater in airway smooth muscle cultured from patients with asthma compared with patients without asthma. This occurs at both the level of mRNA (37.53 ± 11.62- and 13.59 ± 3.12-fold increase at 24 hours compared with time 0, respectively, p < 0.02) and protein production (67.57 ± 27.80- and 3.58 ± 0.6-fold increase at 24 hours compared with time 0, respectively, p < 0.03). The differential connective tissue growth factor response to transforming growth factor-ß in asthmatic airway smooth muscle identifies a potential role for connective tissue growth factor in the remodeling that is characteristic of severe persistent asthma.

Key Words: asthma • extracellular matrix • transforming growth factor-ß




This article has been cited by other articles:


Home page
 Proc Am Thorac SocHome page
B. G. J. Dekkers, H. Maarsingh, H. Meurs, and R. Gosens
Airway Structural Components Drive Airway Smooth Muscle Remodeling in Asthma
Proceedings of the ATS, December 15, 2009; 6(8): 683 - 692.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. Zuyderduyn, M. B. Sukkar, A. Fust, S. Dhaliwal, and J. K. Burgess
Treating asthma means treating airway smooth muscle cells
Eur. Respir. J., August 1, 2008; 32(2): 265 - 274.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
P. G. Woodruff
Gene Expression in Asthmatic Airway Smooth Muscle
Proceedings of the ATS, January 1, 2008; 5(1): 113 - 118.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. van den Brule, J. Heymans, X. Havaux, J.-C. Renauld, D. Lison, F. Huaux, and O. Denis
Profibrotic Effect of IL-9 Overexpression in a Model of Airway Remodeling
Am. J. Respir. Cell Mol. Biol., August 1, 2007; 37(2): 202 - 209.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P. Bradding
Mast cell regulation of airway smooth muscle function in asthma
Eur. Respir. J., May 1, 2007; 29(5): 827 - 830.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. J. Ammit, L. M. Moir, B. G. Oliver, J. M. Hughes, H. Alkhouri, Q. Ge, J. K. Burgess, J. L. Black, and M. Roth
Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L199 - L206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. Borger, M. Tamm, J. L. Black, and M. Roth
Asthma: Is It Due to an Abnormal Airway Smooth Muscle Cell?
Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 367 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
V. Chan, J. K. Burgess, J. C. Ratoff, B. J. O'Connor, A. Greenough, T. H. Lee, and S. J. Hirst
Extracellular Matrix Regulates Enhanced Eotaxin Expression in Asthmatic Airway Smooth Muscle Cells
Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 379 - 385.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. Boxall, S. T. Holgate, and D. E. Davies
The contribution of transforming growth factor-{beta} and epidermal growth factor signalling to airway remodelling in chronic asthma
Eur. Respir. J., January 1, 2006; 27(1): 208 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. R. A. Johnson, J. K. Burgess, Q. Ge, M. Poniris, S. Boustany, S. M. Twigg, and J. L. Black
Connective Tissue Growth Factor Induces Extracellular Matrix in Asthmatic Airway Smooth Muscle
Am. J. Respir. Crit. Care Med., January 1, 2006; 173(1): 32 - 41.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. K. Burgess, Q. Ge, M. H. Poniris, S. Boustany, S. M. Twigg, J. L. Black, and P. R. A. Johnson
Connective tissue growth factor and vascular endothelial growth factor from airway smooth muscle interact with the extracellular matrix
Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L153 - L161.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
J. L. Black, Q. Ge, S. Boustany, P. R. A. Johnson, M. H. Poniris, A. R. Glanville, B. G. G. Oliver, L. M. Moir, and J. K. Burgess
In vitro studies of lymphangioleiomyomatosis
Eur. Respir. J., October 1, 2005; 26(4): 569 - 576.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. E. Brightling, A. J. Ammit, D. Kaur, J. L. Black, A. J. Wardlaw, J. M. Hughes, and P. Bradding
The CXCL10/CXCR3 Axis Mediates Human Lung Mast Cell Migration to Asthmatic Airway Smooth Muscle
Am. J. Respir. Crit. Care Med., May 15, 2005; 171(10): 1103 - 1108.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. M. Kelly, R. Leigh, P. Bonniaud, R. Ellis, J. Wattie, M. J. Smith, G. Martin, M. Panju, M. D. Inman, and J. Gauldie
Epithelial Expression of Profibrotic Mediators in a Model of Allergen-Induced Airway Remodeling
Am. J. Respir. Cell Mol. Biol., February 1, 2005; 32(2): 99 - 107.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Xie, M. B. Sukkar, R. Issa, U. Oltmanns, A. G. Nicholson, and K. F. Chung
Regulation of TGF-{beta}1-induced connective tissue growth factor expression in airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L68 - L76.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
K. Parameswaran, K. Radford, J. Zuo, L.J. Janssen, P.M. O'Byrne, and P.G. Cox
Extracellular matrix regulates human airway smooth muscle cell migration
Eur. Respir. J., October 1, 2004; 24(4): 545 - 551.
[Abstract] [Full Text] [PDF]

Home page
 QJMHome page
S.T. Holgate
Lessons learnt from the epidemic of asthma
QJM, May 1, 2004; 97(5): 247 - 257.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. L. Black
Asthma--More Muscle Cells or More Muscular Cells?
Am. J. Respir. Crit. Care Med., May 1, 2004; 169(9): 980 - 981.
[Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
S. T. Holgate, J. Holloway, S. Wilson, F. Bucchieri, S. Puddicombe, and D. E. Davies
Epithelial-Mesenchymal Communication in the Pathogenesis of Chronic Asthma
Proceedings of the ATS, April 1, 2004; 1(2): 93 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. Tobin
Asthma, Airway Biology, and Nasal Disorders in AJRCCM 2003
Am. J. Respir. Crit. Care Med., January 15, 2004; 169(2): 265 - 276.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2003 American Thoracic Society 		  SOTA, FL