help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Armstrong, D. S.
Right arrow Articles by Grimwood, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Armstrong, D. S.
Right arrow Articles by Grimwood, K.
American Journal of Respiratory and Critical Care Medicine Vol 166. pp. 983-987, (2002)
© 2002 American Thoracic Society


Original Articles

Detection of a Widespread Clone of Pseudomonas aeruginosa in a Pediatric Cystic Fibrosis Clinic

David S. Armstrong, Gillian M. Nixon, Rosemary Carzino, Andrea Bigham, John B. Carlin, Roy M. Robins-Browne and Keith Grimwood

Departments of Respiratory Medicine and Microbiology and Infectious Diseases, Royal Children's Hospital; Clinical Epidemiology and Biostatistics Unit, Murdoch Children's Research Institute, Parkville, Victoria; Department of Paediatrics, Monash Medical Centre, Monash University, Clayton, Victoria, Australia; and Department of Paediatrics and Child Health, Wellington School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand

Correspondence and requests for reprints should be addressed to Dr. David Armstrong, Department of Paediatrics, Monash University, Monash Medical Centre, Clayton Road, Clayton, Victoria 3168, Australia. E-mail: d.armstrong{at}southernhealth.org.au

Cross-infection by Pseudomonas aeruginosa between unrelated patients with cystic fibrosis (CF) is believed to be uncommon. After detecting a genotypically identical strain of P. aeruginosa in five unrelated children with CF dying from severe lung disease, we determined its prevalence within a large CF clinic using pulsed-field gel electrophoresis and random amplified polymorphic DNA assays. The clinical status of P. aeruginosa–infected patients was also determined. Between September and December 1999, 152 patients, aged 3.9–20.7 years, provided sputum for culture. P. aeruginosa was detected in 118 children of mean (SD) age 13.5 (3.8) years. The genotyping techniques were concordant, showing that 65 (55%) infected patients carried an indistinguishable or closely related strain. No distinctive antibiogram or environmental reservoir was found. Patients with the clonal strain were more likely than those with unrelated isolates to have been hospitalized in the preceding 12 months for respiratory exacerbations. This study demonstrates extensive spread of a single, clonal strain of P. aeruginosa in a large pediatric CF clinic. Whether this strain is also more virulent than sporadic isolates remains to be determined. As transmissible strains could emerge elsewhere, other CF clinics may also need to consider molecular methods of surveillance for cross-infection.

Key Words: Pseudomonas aeruginosa • cross-infection • cystic fibrosis




This article has been cited by other articles:


Home page
MicrobiologyHome page
S. M. Kirov, J. S. Webb, C. Y. O'May, D. W. Reid, J. K. K. Woo, S. A. Rice, and S. Kjelleberg
Biofilm differentiation and dispersal in mucoid Pseudomonas aeruginosa isolates from patients with cystic fibrosis
Microbiology, October 1, 2007; 153(10): 3264 - 3274.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Microbiol.Home page
H. Vu-Thien, G. Corbineau, K. Hormigos, B. Fauroux, H. Corvol, A. Clement, G. Vergnaud, and C. Pourcel
Multiple-Locus Variable-Number Tandem-Repeat Analysis for Longitudinal Survey of Sources of Pseudomonas aeruginosa Infection in Cystic Fibrosis Patients
J. Clin. Microbiol., October 1, 2007; 45(10): 3175 - 3183.
[Abstract] [Full Text] [PDF]


Home page
MicrobiologyHome page
D. T. Kenna, C. J. Doherty, J. Foweraker, L. Macaskill, V. A. Barcus, and J. R. W. Govan
Hypermutability in environmental Pseudomonas aeruginosa and in populations causing pulmonary infection in individuals with cystic fibrosis
Microbiology, June 1, 2007; 153(6): 1852 - 1859.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Microbiol.Home page
P. Tingpej, L. Smith, B. Rose, H. Zhu, T. Conibear, K. Al Nassafi, J. Manos, M. Elkins, P. Bye, M. Willcox, et al.
Phenotypic Characterization of Clonal and Nonclonal Pseudomonas aeruginosa Strains Isolated from Lungs of Adults with Cystic Fibrosis
J. Clin. Microbiol., June 1, 2007; 45(6): 1697 - 1704.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
L. Jelsbak, H. K. Johansen, A.-L. Frost, R. Thogersen, L. E. Thomsen, O. Ciofu, L. Yang, J. A. J. Haagensen, N. Hoiby, and S. Molin
Molecular Epidemiology and Dynamics of Pseudomonas aeruginosa Populations in Lungs of Cystic Fibrosis Patients
Infect. Immun., May 1, 2007; 75(5): 2214 - 2224.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
S. Van daele, M. Vaneechoutte, K. De Boeck, C. Knoop, A. Malfroot, P. Lebecque, J. Leclercq-Foucart, L. Van Schil, K. Desager, and F. De Baets
Survey of Pseudomonas aeruginosa genotypes in colonised cystic fibrosis patients
Eur. Respir. J., October 1, 2006; 28(4): 740 - 747.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
D. Chambers, F. Scott, R. Bangur, R. Davies, A. Lim, S. Walters, G. Smith, T. Pitt, D. Stableforth, and D. Honeybourne
Factors associated with infection by Pseudomonas aeruginosa in adult cystic fibrosis
Eur. Respir. J., October 1, 2005; 26(4): 651 - 656.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
A. M. Jones
Eradication therapy for early Pseudomonas aeruginosa infection in CF: many questions still unanswered
Eur. Respir. J., September 1, 2005; 26(3): 373 - 375.
[Full Text] [PDF]


Home page
Arch. Dis. Child.Home page
A Bush
How has research in the last five years changed my clinical practice?
Arch. Dis. Child., August 1, 2005; 90(8): 832 - 836.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
D. A. Lewis, A. Jones, J. Parkhill, D. P. Speert, J. R. W. Govan, J. J. LiPuma, S. Lory, A. K. Webb, and E. Mahenthiralingam
Identification of DNA Markers for a Transmissible Pseudomonas aeruginosa Cystic Fibrosis Strain
Am. J. Respir. Cell Mol. Biol., July 1, 2005; 33(1): 56 - 64.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Microbiol.Home page
S. Van daele, R. Verhelst, G. Claeys, G. Verschraegen, H. Franckx, L. Van Simaey, C. de Ganck, F. De Baets, and M. Vaneechoutte
Shared Genotypes of Achromobacter xylosoxidans Strains Isolated from Patients at a Cystic Fibrosis Rehabilitation Center
J. Clin. Microbiol., June 1, 2005; 43(6): 2998 - 3002.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
A. L. Griffiths, K. Jamsen, J. B. Carlin, K. Grimwood, R. Carzino, P. J. Robinson, J. Massie, and D. S. Armstrong
Effects of Segregation on an Epidemic Pseudomonas aeruginosa Strain in a Cystic Fibrosis Clinic
Am. J. Respir. Crit. Care Med., May 1, 2005; 171(9): 1020 - 1025.
[Abstract] [Full Text] [PDF]


Home page
JAMAHome page
Z. Li, M. R. Kosorok, P. M. Farrell, A. Laxova, S. E. H. West, C. G. Green, J. Collins, M. J. Rock, and M. L. Splaingard
Longitudinal Development of Mucoid Pseudomonas aeruginosa Infection and Lung Disease Progression in Children With Cystic Fibrosis
JAMA, February 2, 2005; 293(5): 581 - 588.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
A. M. Jones, M. E. Dodd, J. R. W. Govan, C. J. Doherty, C. M. Smith, B. J. Isalska, and A. K. Webb
Prospective Surveillance for Pseudomonas aeruginosa Cross-Infection at a Cystic Fibrosis Center
Am. J. Respir. Crit. Care Med., February 1, 2005; 171(3): 257 - 260.
[Abstract] [Full Text] [PDF]


Home page
J Med MicrobiolHome page
M. W Syrmis, M. R O'Carroll, T. P Sloots, C. Coulter, C. E Wainwright, S. C Bell, and M. D Nissen
Rapid genotyping of Pseudomonas aeruginosa isolates harboured by adult and paediatric patients with cystic fibrosis using repetitive-element-based PCR assays
J. Med. Microbiol., November 1, 2004; 53(11): 1089 - 1096.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
S.D. Aaron, D. Kottachchi, W.J. Ferris, K.L. Vandemheen, M.L. St. Denis, A. Plouffe, S.P. Doucette, R. Saginur, F.T. Chan, and K. Ramotar
Sputum versus bronchoscopy for diagnosis of Pseudomonas aeruginosa biofilms in cystic fibrosis
Eur. Respir. J., October 1, 2004; 24(4): 631 - 637.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
A.L. Griffiths, D. Armstrong, R. Carzino, and P. Robinson
Cystic fibrosis patients and families support cross-infection measures
Eur. Respir. J., September 1, 2004; 24(3): 449 - 452.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
M.R. O'Carroll, M.W. Syrmis, C.E. Wainwright, R.M. Greer, P. Mitchell, C. Coulter, T.P. Sloots, M.D. Nissen, and S.C. Bell
Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units
Eur. Respir. J., July 1, 2004; 24(1): 101 - 106.
[Abstract] [Full Text] [PDF]


Home page
J Med MicrobiolHome page
F. W. Scott and T. L. Pitt
Identification and characterization of transmissible Pseudomonas aeruginosa strains in cystic fibrosis patients in England and Wales
J. Med. Microbiol., July 1, 2004; 53(7): 609 - 615.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
D.W. Reid, A. Champion, R. Bradbury, and S.M. Kirov
Host response to transmissible Pseudomonas aeruginosa
Eur. Respir. J., March 1, 2004; 23(3): 493 - 494.
[Full Text] [PDF]


Home page
J. Clin. Microbiol.Home page
P. Robinson, R. Carzino, D. Armstrong, and A. Olinsky
Pseudomonas Cross-Infection from Cystic Fibrosis Patients to Non-Cystic Fibrosis Patients: Implications for Inpatient Care of Respiratory Patients
J. Clin. Microbiol., December 1, 2003; 41(12): 5741 - 5741.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
P. M. Farrell, Z. Li, M. R. Kosorok, A. Laxova, C. G. Green, J. Collins, H.-C. Lai, M. J. Rock, and M. L. Splaingard
Bronchopulmonary Disease in Children with Cystic Fibrosis after Early or Delayed Diagnosis
Am. J. Respir. Crit. Care Med., November 1, 2003; 168(9): 1100 - 1108.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
R. L. Gibson, J. L. Burns, and B. W. Ramsey
Pathophysiology and Management of Pulmonary Infections in Cystic Fibrosis
Am. J. Respir. Crit. Care Med., October 15, 2003; 168(8): 918 - 951.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
A.M. Jones, L. Martin, R.J. Bright-Thomas, M.E. Dodd, A McDowell, K.L. Moffitt, J.S. Elborn, and A.K. Webb
Inflammatory markers in cystic fibrosis patients with transmissible Pseudomonas aeruginosa
Eur. Respir. J., September 1, 2003; 22(3): 503 - 506.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Microbiol.Home page
D. Armstrong, S. Bell, M. Robinson, P. Bye, B. Rose, C. Harbour, C. Lee, H. Service, M. Nissen, M. Syrmis, et al.
Evidence for Spread of a Clonal Strain of Pseudomonas aeruginosa among Cystic Fibrosis Clinics
J. Clin. Microbiol., May 1, 2003; 41(5): 2266 - 2267.
[Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
M. J. Tobin
Pediatrics, Surfactant, and Cystic Fibrosis in AJRCCM 2002
Am. J. Respir. Crit. Care Med., February 1, 2003; 167(3): 333 - 344.
[Full Text] [PDF]


Home page
JWatch Infect. DiseasesHome page
Pseudomonas Colonization in CF: Environmental or Person-to-Person Spread?
Journal Watch Infectious Diseases, October 25, 2002; 2002(1025): 10 - 10.
[Full Text]


Home page
Am. J. Respir. Crit. Care Med.Home page
B. W. Ramsey
To Cohort or Not to Cohort: How Transmissible Is Pseudomonas aeruginosa?
Am. J. Respir. Crit. Care Med., October 1, 2002; 166(7): 906 - 907.
[Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2002 American Thoracic Society