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Inflammatory Mediator mRNA Expression by Adenovirus E1A-Transfected Bronchial Epithelial Cells

University of British Columbia, McDonald Research Laboratory, St. Paul's Hospital, Vancouver, British Columbia, Canada

Correspondence and requests for reprints should be addressed to Shizu Hayashi, University of British Columbia McDonald Research Laboratory, St. Paul's Hospital, 1081 Burrard Street, Vancouver, BC, V6Z 1Y6 Canada. E-mail: shayashi{at}mrl.ubc.ca

Lung tissue from patients with emphysema and airway obstruction carries excess adenoviral E1A DNA that is expressed as protein in airway surface epithelium and is associated with an increased inflammatory response. To examine mechanisms by which latent adenoviral infection might amplify the inflammatory process, we transfected primary human bronchial epithelial (HBE) cells from three separate patients undergoing lung resection so that they stably expressed adenovirus E1A. Lipopolysaccharide stimulation of the E1A-transfected HBE cells increased intercellular adhesion molecule-1 and interleukin-8 mRNA and protein expression compared with control cells from the same patient. It also induced greater intercellular adhesion molecule-1 promoter activity and greater nuclear factor-B binding activity of nuclear extracts in E1A transfectants than controls. E1A-positive transfectants constitutively expressed transforming growth factor-ß1 mRNA and protein, whereas this expression was either very low or not detected in control cells. We conclude that adenoviral E1A tranfection transforms primary HBE cells and upregulates their production of mediators that are clinically relevant to the pathogenesis of chronic obstructive pulmonary disease.

Key Words: intercellular adhesion molecule-1 • interleukin-8 • transforming growth factor-ß • adenovirus E1A proteins • lipopolysaccharides

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