This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tsuchiya, T. Articles by Nakamura, H. Search for Related Content PubMed PubMed Citation Articles by Tsuchiya, T. Articles by Nakamura, H.

Dendritic Cell Involvement in Pulmonary Granuloma Formation Elicited by Bacillus Calmette-Guérin in Rats

Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan; and Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts

Correspondence and requests for reprints should be addressed to Takafumi Suda, M.D., Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, Shizuoka, 431-3192 Japan. E-mail: suda{at}hama-med.ac.jp

Dendritic cells (DCs) are the most potent antigen-presenting cells that play a central role in initiating the primary immune response. However, their role in granulomatous inflammation has not been well studied. The aim of the present study was to elucidate the role of DCs in granuloma formation. Using a rat model of bacillus Calmette-Guérin (BCG)-elicited pulmonary granulomas, we investigated the distribution of DCs in the granulomas by immunohistochemistry with a rat-DC–specific monoclonal antibody, OX62. We found numerous large, pleiomorphic OX62⁺ cells accumulating at the borders of the pulmonary granulomas. The OX62⁺ cells isolated from the granulomatous lung showed intense surface expression of major histocompatibility complex class II, B7–1, and B7–2, and a lack of T cell– and monocyte/macrophage-specific markers. Their ultrastructural morphology was characteristic of DCs. Functionally, they had potent capacity to stimulate allogeneic T cells as well as purified protein derivative-specific syngeneic T cells in the absence of exogenous peptides. Based on these findings, the OX62⁺ cells infiltrating the granulomas were considered to be DCs expressing BCG-derived peptides. These results indicate that DCs contribute to pulmonary granuloma formation elicited by BCG by means of their potent antigen-presenting function, providing a novel insight into DC function in T cell–mediated granulomatous immune responses.

Key Words: dendritic cells • granuloma • BCG

This article has been cited by other articles:

				M. A.M. Willart, H. Jan de Heer, H. Hammad, T. Soullie, K. Deswarte, B. E. Clausen, L. Boon, H. C. Hoogsteden, and B. N. Lambrecht The lung vascular filter as a site of immune induction for T cell responses to large embolic antigen J. Exp. Med., November 23, 2009; 206(12): 2823 - 2835. [Abstract] [Full Text] [PDF]

				Y. Ozawa, T. Suda, T. Nagata, D. Hashimoto, Y. Nakamura, N. Enomoto, N. Inui, Y. Koide, H. Nakamura, and K. Chida Mucosal Vaccine Using CTL Epitope-Pulsed Dendritic Cell Confers Protection for Intracellular Pathogen Am. J. Respir. Cell Mol. Biol., October 1, 2009; 41(4): 440 - 448. [Abstract] [Full Text] [PDF]

				L. Lei, B. L. Plattner, and J. M. Hostetter Live Mycobacterium avium subsp. paratuberculosis and a Killed-Bacterium Vaccine Induce Distinct Subcutaneous Granulomas, with Unique Cellular and Cytokine Profiles Clin. Vaccine Immunol., May 1, 2008; 15(5): 783 - 793. [Abstract] [Full Text] [PDF]

				T. Naito, T. Suda, K. Suzuki, Y. Nakamura, N. Inui, J. Sato, K. Chida, and H. Nakamura Lung Dendritic Cells Have a Potent Capability to Induce Production of Immunoglobulin A Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 161 - 167. [Abstract] [Full Text] [PDF]

				Yimin and M. Kohanawa A Regulatory Effect of the Balance between TNF-{alpha} and IL-6 in the Granulomatous and Inflammatory Response to Rhodococcus aurantiacus Infection in Mice J. Immunol., July 1, 2006; 177(1): 642 - 650. [Abstract] [Full Text] [PDF]

				S Stenger Immunological control of tuberculosis: role of tumour necrosis factor and more Ann Rheum Dis, November 1, 2005; 64(suppl_4): iv24 - iv28. [Abstract] [Full Text] [PDF]

				M. Buettner, C. Meinken, M. Bastian, R. Bhat, E. Stossel, G. Faller, G. Cianciolo, J. Ficker, M. Wagner, M. Rollinghoff, et al. Inverse Correlation of Maturity and Antibacterial Activity in Human Dendritic Cells J. Immunol., April 1, 2005; 174(7): 4203 - 4209. [Abstract] [Full Text] [PDF]

				K. Kawamura, K. Iyonaga, H. Ichiyasu, J. Nagano, M. Suga, and Y. Sasaki Differentiation, Maturation, and Survival of Dendritic Cells by Osteopontin Regulation Clin. Vaccine Immunol., January 1, 2005; 12(1): 206 - 212. [Abstract] [Full Text] [PDF]

				J. Morimoto, M. Inobe, C. Kimura, S. Kon, H. Diao, M. Aoki, T. Miyazaki, D. T. Denhardt, S. Rittling, and T. Uede Osteopontin affects the persistence of {beta}-glucan-induced hepatic granuloma formation and tissue injury through two distinct mechanisms Int. Immunol., March 1, 2004; 16(3): 477 - 488. [Abstract] [Full Text] [PDF]

				B.-C. Chiu, C. M. Freeman, V. R. Stolberg, J. S. Hu, E. Komuniecki, and S. W. Chensue The Innate Pulmonary Granuloma: Characterization and Demonstration of Dendritic Cell Recruitment and Function Am. J. Pathol., March 1, 2004; 164(3): 1021 - 1030. [Abstract] [Full Text] [PDF]

				M. J. Tobin Tuberculosis, Lung Infections, Interstitial Lung Disease, and Journalology in AJRCCM 2002 Am. J. Respir. Crit. Care Med., February 1, 2003; 167(3): 345 - 355. [Full Text] [PDF]

				D. Dreher and L. P. Nicod Dendritic Cells in the Mycobacterial Granuloma Are Involved in Acquired Immunity Am. J. Respir. Crit. Care Med., June 15, 2002; 165(12): 1577 - 1578. [Full Text] [PDF]