This Article Full Text Full Text (PDF) Online Data Supplement Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fukuse, T. Articles by Wada, H. Search for Related Content PubMed PubMed Citation Articles by Fukuse, T. Articles by Wada, H.

Effect of Adenosine Triphosphate–Sensitive Potassium Channel Openers on Lung Preservation

Department of Thoracic Surgery, Otsu Red-Cross Hospital, Otsu-City; and Department of Thoracic Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan

Correspondence and requests for reprints should be addressed to Tatsuo Fukuse, M.D., Department of Thoracic Surgery, Otsu Red-Cross Hospital, 1-1-35 Nagara, Otsu-City 520-8511, Japan. E-mail: tafukuse{at}f4.dion.ne.jp

ATP-sensitive potassium channel (KATP) openers have been proven to be involved in ischemic preconditioning, which protects ischemic tissue. However, the effect of KATP openers on ischemia–reperfusion injury of the lungs remains unknown. We investigated whether a KATP opener, pinacidil, can attenuate ischemia–reperfusion injury using an ex vivo rat lung model. Heart–lung blocks were flushed and preserved with phosphate-buffered saline (control group) or with one of the solutions containing pinacidil (pinacidil group) or pinacidil + glibenclamide (a KATP blocker) (glibenclamide group). The control and glibenclamide groups showed significantly higher values with respect to shunt fraction, pulmonary arterial pressure, and peak inspiratory pressure than the pinacidil group. The concentrations of total adenine nucleotides and ATP in the lung after reperfusion became significantly lower in the control and glibenclamide groups than in the fresh group. Lipid peroxidation of the lungs increased significantly in the control and glibenclamide groups after reperfusion. State 3 mitochondrial respiration and State 3/4 ratios of mitochondrial respiration were significantly decreased in the lungs of the control and glibenclamide groups. These findings suggested that KATP openers would maintain the mitochondrial respiratory function during lung preservation, prevent lipid peroxidation after reperfusion, and attenuate ischemia–reperfusion injury.

Key Words: ATP-sensitive potassium channel openers • lung preservation • reperfusion injury • mitochondria • ATP

This article has been cited by other articles:

				C. Leroy, A. Dagenais, Y. Berthiaume, and E. Brochiero Molecular identity and function in transepithelial transport of KATP channels in alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L1027 - L1037. [Abstract] [Full Text] [PDF]

				M. de Perrot, M. Liu, T. K. Waddell, and S. Keshavjee Ischemia-Reperfusion-induced Lung Injury Am. J. Respir. Crit. Care Med., February 15, 2003; 167(4): 490 - 511. [Abstract] [Full Text] [PDF]

				J. D. McCully and S. Levitsky The mitochondrial KATP channel and cardioprotection Ann. Thorac. Surg., February 1, 2003; 75(2): S667 - 673. [Abstract] [Full Text] [PDF]

				M. J. Tobin Chronic Obstructive Pulmonary Disease, Pollution, Pulmonary Vascular Disease, Transplantation, Pleural Disease, and Lung Cancer in AJRCCM 2002 Am. J. Respir. Crit. Care Med., February 1, 2003; 167(3): 356 - 370. [Full Text] [PDF]