Am. J. Respir. Crit. Care Med., Volume 164, Number 8, October 2001, 1501-1507

Differential Role of CD80 and CD86 Molecules in the Induction and the Effector Phases of Allergic Rhinitis in Mice

MITSUHIRO OKANO, MIYUKI AZUMA, TADASHI YOSHINO, HISASHI HATTORI, MICHIHIRO NAKADA, ABHAY R. SATOSKAR, DONALD A. HARN Jr, EIICHI NAKAYAMA, TADAATSU AKAGI, and KAZUNORI NISHIZAKI

Department of Otolaryngology, Pathology, Immunology, and Parasitology, Okayama University Medical School, Okayama, Japan; Department of Molecular Immunology, Division of Oral Health Sciences Graduate School, Tokyo Medical and Dental University, Tokyo, Japan; and Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts

Selective involvement of CD80 and/or CD86 in the differentiation of T-helper (Th)1 and Th2 was seen in several diseases. In this study, we sought to determine the differential roles of CD80 and CD86 in the induction and effector phase of allergic rhinitis using Schistosoma mansoni egg antigen (SEA) as a specific Ag. Intranasal sensitization with SEA in BALB/c mice elicited a strong Th2 response including SEA-specific IgE production, nasal eosinophilia, and IL-4 and IL-5 production by nasal lymphocytes after Ag challenge. Blockade of CD80 at the induction phase significantly inhibited these manifestations, whereas no effect was observed by CD86 blockade. In contrast, the simultaneous blockade of both CD80 and CD86 during the effector phase partially inhibited IgE and IgG₁ production and nasal eosinophilia, although either blockade of CD80 or CD86 during the phase failed to inhibit these responses. Flow cytometric analysis on nasal mononuclear cells showed that CD80 but not CD86 was preferentially expressed on non-B cells by in vitro SEA stimulation in unsensitized mice. However, both CD80 and CD86 expression were significantly augmented by in vitro SEA stimulation in sensitized mice. Our results suggest the differential roles and expression of CD80 and CD86 in the development of allergic rhinitis.

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