help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SAGEL, S. D.
Right arrow Articles by ACCURSO, F. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SAGEL, S. D.
Right arrow Articles by ACCURSO, F. J.

Am. J. Respir. Crit. Care Med., Volume 164, Number 8, October 2001, 1425-1431

Airway Inflammation in Children with Cystic Fibrosis and Healthy Children Assessed by Sputum Induction

SCOTT D. SAGEL, ROBERT KAPSNER, IRIS OSBERG, MARCI K. SONTAG, and FRANK J. ACCURSO

Department of Pediatrics, University of Colorado Health Sciences Center, Denver, Colorado

A noninvasive method to characterize inflammation and infection in the airways of nonexpectorating children with cystic fibrosis (CF) is needed for clinical and research purposes. Accordingly, we performed sputum inductions by administering 3% saline to 11 healthy control children and 20 children with CF, composed of 7 sputum producers (capable of spontaneously expectorating sputum) and 13 nonproducers. Induced sputum weights were comparable in each group, whereas the amount of induced sputum collected from the CF producers was over 10-fold higher than the spontaneously expectorated samples. We found a significant increase in indices of airway inflammation, including total cell counts, absolute neutrophil counts, interleukin-8 (IL-8) levels, and neutrophil elastase activity in the CF subjects compared with the healthy control subjects. These same indices in the induced sputum specimens from CF producers were significantly correlated with levels in the matched expectorated sputum specimens. Sputum total protein concentration was elevated in the CF groups, whereas urea and albumin levels were not significantly different. Salivary analysis, performed separately, revealed higher levels of IL-8 and total protein in the CF groups. Airway infection, as assessed by quantitative counts of CF-related bacterial pathogens, was also higher in the CF subjects. The same bacterial pathogens, in similar colony counts, were isolated from both the induced and expectorated sputum samples from the CF producers. We conclude that airway inflammation and infection, assessed through sputum induction, are significantly increased in children with CF as compared with healthy children. Furthermore, induced sputum samples are similar to spontaneously expectorated samples in describing both inflammation and infection in the CF airway.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Perez, A. M. van Heeckeren, D. Nichols, S. Gupta, J. F. Eastman, and P. B. Davis
Peroxisome proliferator-activated receptor-{gamma} in cystic fibrosis lung epithelium
Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L303 - L313.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
K J Mackerness, G R Jenkins, A Bush, and P J Jose
Characterisation of the range of neutrophil stimulating mediators in cystic fibrosis sputum
Thorax, July 1, 2008; 63(7): 614 - 620.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. M. Tunney, T. R. Field, T. F. Moriarty, S. Patrick, G. Doering, M. S. Muhlebach, M. C. Wolfgang, R. Boucher, D. F. Gilpin, A. McDowell, et al.
Detection of Anaerobic Bacteria in High Numbers in Sputum from Patients with Cystic Fibrosis
Am. J. Respir. Crit. Care Med., May 1, 2008; 177(9): 995 - 1001.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
H. Mussaffi, E. M. Fireman, M. Mei-Zahav, D. Prais, and H. Blau
Induced Sputum in the Very Young: A New Key to Infection and Inflammation
Chest, January 1, 2008; 133(1): 176 - 182.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
S. D. Sagel, J. F. Chmiel, and M. W. Konstan
Sputum Biomarkers of Inflammation in Cystic Fibrosis Lung Disease
Proceedings of the ATS, August 1, 2007; 4(4): 406 - 417.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Gaggar, Y. Li, N. Weathington, M. Winkler, M. Kong, P. Jackson, J. E. Blalock, and J. P. Clancy
Matrix metalloprotease-9 dysregulation in lower airway secretions of cystic fibrosis patients
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L96 - L104.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Mayer-Hamblett, M. L. Aitken, F. J. Accurso, R. A. Kronmal, M. W. Konstan, J. L. Burns, S. D. Sagel, and B. W. Ramsey
Association between Pulmonary Function and Sputum Biomarkers in Cystic Fibrosis
Am. J. Respir. Crit. Care Med., April 15, 2007; 175(8): 822 - 828.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
C. H Goss and J. L Burns
Exacerbations in cystic fibrosis {middle dot} 1: Epidemiology and pathogenesis
Thorax, April 1, 2007; 62(4): 360 - 367.
[Abstract] [Full Text] [PDF]

Home page
 The Annals of PharmacotherapyHome page
L. M Taylor and R. J Kuhn
Hypertonic Saline Treatment of Cystic Fibrosis
Ann. Pharmacother., March 1, 2007; 41(3): 481 - 484.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
A Clement, A Tamalet, E Leroux, S Ravilly, B Fauroux, and J-P Jais
Long term effects of azithromycin in patients with cystic fibrosis: a double blind, placebo controlled trial
Thorax, October 1, 2006; 61(10): 895 - 902.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child.Home page
S Rao and J Grigg
New insights into pulmonary inflammation in cystic fibrosis.
Arch. Dis. Child., September 1, 2006; 91(9): 786 - 788.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. J. Sloane, R. A. Lindner, S. S. Prasad, L. T. Sebastian, S. K. Pedersen, M. Robinson, P. T. Bye, D. W. Nielson, and J. L. Harry
Proteomic Analysis of Sputum from Adults and Children with Cystic Fibrosis and from Control Subjects
Am. J. Respir. Crit. Care Med., December 1, 2005; 172(11): 1416 - 1426.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. L. Lee, R. C. Rancourt, G. del Val, K. Pack, C. Pardee, F. J. Accurso, and C. W. White
Thioredoxin and dihydrolipoic acid inhibit elastase activity in cystic fibrosis sputum
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L875 - L882.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. McAllister, A. Henry, J. L. Kreindler, P. J. Dubin, L. Ulrich, C. Steele, J. D. Finder, J. M. Pilewski, B. M. Carreno, S. J. Goldman, et al.
Role of IL-17A, IL-17F, and the IL-17 Receptor in Regulating Growth-Related Oncogene-{alpha} and Granulocyte Colony-Stimulating Factor in Bronchial Epithelium: Implications for Airway Inflammation in Cystic Fibrosis
J. Immunol., July 1, 2005; 175(1): 404 - 412.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Linden, M. Laan, and G. P. Anderson
Neutrophils, interleukin-17A and lung disease
Eur. Respir. J., January 1, 2005; 25(1): 159 - 172.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. J. Marshall, B. Perks, K. Bodey, R. Suri, A. Bush, and J. K. Shute
Free Secretory Component from Cystic Fibrosis Sputa Displays the Cystic Fibrosis Glycosylation Phenotype
Am. J. Respir. Crit. Care Med., February 1, 2004; 169(3): 399 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. S. Armstrong
In Celebration of Expectoration: Induced Sputum Indices as Outcome Measures in Cystic Fibrosis
Am. J. Respir. Crit. Care Med., December 15, 2003; 168(12): 1412 - 1413.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. L. Ordonez, N. R. Henig, N. Mayer-Hamblett, F. J. Accurso, J. L. Burns, J. F. Chmiel, C. L. Daines, R. L. Gibson, S. McNamara, G. Z. Retsch-Bogart, et al.
Inflammatory and Microbiologic Markers in Induced Sputum after Intravenous Antibiotics in Cystic Fibrosis
Am. J. Respir. Crit. Care Med., December 15, 2003; 168(12): 1471 - 1475.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. L. Gibson, J. L. Burns, and B. W. Ramsey
Pathophysiology and Management of Pulmonary Infections in Cystic Fibrosis
Am. J. Respir. Crit. Care Med., October 15, 2003; 168(8): 918 - 951.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
L. Saiman, B. C. Marshall, N. Mayer-Hamblett, J. L. Burns, A. L. Quittner, D. A. Cibene, S. Coquillette, A. Y. Fieberg, F. J. Accurso, and P. W. Campbell III
Azithromycin in Patients With Cystic Fibrosis Chronically Infected With Pseudomonas aeruginosa: A Randomized Controlled Trial
JAMA, October 1, 2003; 290(13): 1749 - 1756.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
N. Reinhardt, C.I.U. Chen, D. Loppow, T. Schink, I. Kleinau, R.A. Jorres, U. Wahn, H. Magnussen, and K.P. Paul
Cellular profiles of induced sputum in children with stable cystic fibrosis: comparison with BAL
Eur. Respir. J., September 1, 2003; 22(3): 497 - 502.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
G. E. Carpagnano, P. J. Barnes, D. M. Geddes, M. E. Hodson, and S. A. Kharitonov
Increased Leukotriene B4 and Interleukin-6 in Exhaled Breath Condensate in Cystic Fibrosis
Am. J. Respir. Crit. Care Med., April 15, 2003; 167(8): 1109 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. L. Gibson, J. Emerson, S. McNamara, J. L. Burns, M. Rosenfeld, A. Yunker, N. Hamblett, F. Accurso, M. Dovey, P. Hiatt, et al.
Significant Microbiological Effect of Inhaled Tobramycin in Young Children with Cystic Fibrosis
Am. J. Respir. Crit. Care Med., March 15, 2003; 167(6): 841 - 849.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. P. Rooney, G. M. Denning, B. P. Davis, D. M. Flaherty, J. A. Chiorini, and J. Zabner
Bronchoalveolar Fluid Is Not a Major Hindrance to Virus-Mediated Gene Therapy in Cystic Fibrosis
J. Virol., September 11, 2002; 76(20): 10437 - 10443.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Pediatrics, Surfactant, and Cystic Fibrosis in AJRCCM 2001
Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 619 - 630.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2001 American Thoracic Society