Am. J. Respir. Crit. Care Med., Volume 164, Number 5, September 2001, 754-758

Effects of Oral Steroids on Blood CXCR3+ and CCR4+ T Cells in Patients with Bronchial Asthma

KAZUYOSHI KURASHIMA, MASAKI FUJIMURA, SHIGEHARU MYOU, KAZUO KASAHARA, HIDEKI TACHIBANA, NORINAO AMEMIYA, YOSHIHISA ISHIURA, NOBUYUKI ONAI, KOUJI MATSUSHIMA, and SHINJI NAKAO

Third Department of Internal Medicine, School of Medicine, Kanazawa University; and Department of Molecular Preventive Medicine, School of Medicine, Tokyo University

Corticosteroids are widely used in bronchial asthma, but their mechanism of action is not fully understood. The in vitro studies have proposed that human T helper cells, type 1 (Th1) favor expression of CXCR3, whereas Th2 cells favor CCR4. In this study we investigated whether oral prednisolone modulates the balance of peripheral blood CXCR3+ and CCR4+ T cells. We analyzed the T-cell subsets in 28 patients with stable atopic asthma and 13 normal control subjects before and after 2 wk of treatment with prednisolone, 20 mg/d, or placebo in a randomized, double-blind, parallel group study. The numbers of CXCR3+ and CCR4+ memory T cells were measured with a flow cytometer, and expressed as percentages in CD4+/CD45RO+ memory T cells. In the steroid-treated asthma group, there was a decrease in CCR4+ T cells (from 29.3% to 20.3%, p < 0.0001), and an increase in CXCR3+/ CCR4+ ratio (from 1.86 to 2.89, p = 0.0047), whereas there was no change in CXCR3+ T cells. However, the percentages of CCR4+ cells did not change after steroid therapy in normal control subjects. These results suggest that short-term oral corticosteroid modulates the balances of CXCR3+ and CCR4+ cells in patients with asthma.

Keywords: asthma; steroid; chemokine; receptor; blood

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