Am. J. Respir. Crit. Care Med., Volume 164, Number 4, August 2001, 575-579

The Kinetics of Allergen-induced Eotaxin Level in Nasal Lavage Fluid
Its Key Role in Eosinophil Recruitment in Nasal Mucosa

NOBUHISA TERADA, NANAKO HAMANO, WOO JEONG KIM, KOICHI HIRAI, TOSHIHARU NAKAJIMA, HIROKAZU YAMADA, HIROSHI KAWASAKI, TETSUJI YAMASHITA, HIROHISA KISHI, TOMOHIRO NOMURA, TSUTOMU NUMATA, OSAMU YOSHIE, and AKIYOSHI KONNO

Department of Otorhinolaryngology, Chiba University School of Medicine, Chiba, Japan; Department of Bioregulatory Function and Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine, Tokyo, Japan; Division of Clinical Immunology and Immunotherapeutics, Institute of Medical Science Hospital, University of Tokyo, Tokyo, Japan; R&D Mitsubisi Kagaku Bio-clinical Laboratories, Inc., Tokyo, Japan; and Department of Microbiology, Kinki University School of Medicine, Osaka Sayama, Japan

Eotaxin (CCL11) is a potent eosinophil chemoattractant belonging to the C-C chemokine. To evaluate the role of eotaxin in eosinophilic inflammation in nasal mucosa, we investigated the levels of eosinophil chemoattractants in nasal lavage fluids obtained after antigen challenge, compared with eosinophil counts and eosinophil protein X (EPX) levels. In subjects with allergic rhinitis, allergen challenge led to parallel increases in eosinophil counts, levels of EPX, and eotaxin concentrations in nasal lavage fluid. The levels of eotaxin in lavage samples showed strong correlation with lavage levels of eosinophil counts and EPX. Normal subjects had few, if any, eosinophils and EPX as well as the measured parameters in their nasal lavage fluids before and after antigen challenge. In our experiments of eosinophil endothelial transmigration (TEM) assay using the nasal microvascular endothelial cells, eotaxin showed the most potent effect among various eosinophil chemoattractants. In addition, treatment of eosinophils with anti-CCR-3 mAb significantly blocked eosinophil TEM induced by homogenate of nasal mucosa. These results indicate that eotaxin has an important role in eosinophil-dependent inflammation in nasal mucosa and suggest that blocking eotaxin or CCR-3 might be useful for new therapeutic tools of allergic rhinitis.

Keywords: eotaxin; eosinophil; allergic rhinitis; nasal lavage

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