Am. J. Respir. Crit. Care Med., Volume 164, Number 11, December 2001, 2114-2119

Nontypeable Haemophilus influenzae in the Lower Respiratory Tract of Patients with Chronic Bronchitis

VENKATA BANDI, MICHAEL A. APICELLA, EDWARD MASON, TIMOTHY F. MURPHY, ATHER SIDDIQI, ROBERT L. ATMAR, and STEPHEN B. GREENBERG

Departments of Medicine, Molecular Virology and Microbiology, and Pediatrics, Baylor College of Medicine, Houston, Texas; Ben Taub General Hospital, Houston, Texas; Department of Microbiology, University of Iowa School of Medicine, Iowa City, Iowa; and Department of Medicine, State University of New York at Buffalo, Buffalo, New York

The frequency of colonization and intracellular localization of nontypeable Haemophilus influenzae (NTHi) in the lower respiratory tract was determined in healthy adults and in clinically stable and acutely ill chronic bronchitis (CB) patients. NTHi was recovered from bronchial wash or bronchial brush specimens in 6 of 23 (26%) stable CB patients and in 1 of 15 (7%) CB patients with a respiratory exacerbation. No NTHi (0 of 26) was recovered from lower tract specimens of healthy adults undergoing anesthesia for elective surgery. Molecular typing of NTHi strains revealed that five of nine patients with stable CB had different strains in upper respiratory tract and bronchial wash/brush specimens collected simultaneously. Four stable patients with CB had different strains recovered on repeat bronchoscopy. These results demonstrate the frequent colonization of the lower airways of stable CB patients with multiple strains of NTHi. Bronchial biopsies also were examined for intracellular NTHi by in situ hybridization and immunofluorescence microscopy. Intracellular NTHi were found in 0 of 7 healthy adults, 8 of 24 patients with clinically stable CB, and 13 of 15 acutely ill CB patients. This observation suggests a role for intracellular infection by NTHi in the pathogenesis of exacerbations of CB.

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