help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SEYMOUR, M. L.
Right arrow Articles by SAMPSON, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SEYMOUR, M. L.
Right arrow Articles by SAMPSON, A. P.

Am. J. Respir. Crit. Care Med., Volume 164, Number 11, December 2001, 2051-2056

Leukotriene and Prostanoid Pathway Enzymes in Bronchial Biopsies of Seasonal Allergic Asthmatics

MICHELLE L. SEYMOUR, SABINA RAK, DANIEL ÅBERG, GERT C. RIISE, JOHN F. PENROSE, YOSHIHIDE KANAOKA, K. FRANK AUSTEN, STEPHEN T. HOLGATE, and ANTHONY P. SAMPSON

Division of Respiratory Cell & Molecular Biology, Southampton General Hospital, Southampton, United Kingdom; Allergy Centre, Sahlgrenska University Hospital, Gothenberg, Sweden; and Department of Medicine, Harvard Medical School, Boston, Massachusetts

Cysteinyl-leukotrienes and prostaglandin D2 generated by the 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathways, respectively, cause bronchoconstriction, leukocyte recruitment, and bronchial hyperresponsiveness in asthma. We characterized the cellular expression of 5-LO and COX enzymes using immunohistochemistry on bronchial biopsies from 12 allergic asthmatic patients before and during seasonal exposure to birch pollen. Bronchial responsiveness (p = 0.004) and symptoms (p < 0.005) increased and peak expiratory flow (PEF; p =< 0.02) decreased in the pollen season. In-season biopsies had 2-fold more cells immunostaining for 5-LO (p = 0.02), 5-LO-activating protein (FLAP; p = 0.04), and leukotriene (LT)A4 hydrolase (p = 0.05), and 4-fold more for the terminal enzyme for cysteinyl-leukotriene synthesis, LTC4 synthase (p = 0.02). Immunostaining for COX-1, COX-2, and PGD2 synthase was unchanged. Increased staining for LTC4 synthase was due to increased eosinophils (p = 0.035) and an increased proportion of eosinophils expressing the enzyme (p = 0.047). Macrophages also increased (p = 0.019), but mast cells and T-lymphocyte subsets were unchanged. Inverse correlations between PEF and 5-LO+ cell counts link increased expression of 5-LO pathway enzymes in eosinophils and macrophages within the bronchial mucosa to deterioration of lung function during seasonal allergen exposure.




This article has been cited by other articles:


Home page
 Arch Otolaryngol Head Neck SurgHome page
J. M. Owens, K. R. Shroyer, and T. T. Kingdom
Expression of cyclooxygenase and lipoxygenase enzymes in nasal polyps of aspirin-sensitive and aspirin-tolerant patients.
Arch Otolaryngol Head Neck Surg, June 1, 2006; 132(6): 579 - 587.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. Kikuchi, S. Kikuchi, T. Kobayashi, K. Hagiwara, Y. Sakamoto, M. Kanazawa, and M. Nagata
Eosinophil Trans-Basement Membrane Migration Induced by Interleukin-8 and Neutrophils
Am. J. Respir. Cell Mol. Biol., June 1, 2006; 34(6): 760 - 765.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Wang, P. Chitano, and T. M. Murphy
Length oscillation induces force potentiation in infant guinea pig airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L909 - L915.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Cai, L. Bjermer, and T. S. Halstensen
Bronchial Mast Cells Are the Dominating LTC4S-Expressing Cells in Aspirin-Tolerant Asthma
Am. J. Respir. Cell Mol. Biol., December 1, 2003; 29(6): 683 - 693.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. Tobin
Compliance (COMmunicate PLease wIth Less Abbreviations, Noun Clusters, and Exclusiveness)
Am. J. Respir. Crit. Care Med., December 15, 2002; 166(12): 1534 - 1536.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Asthma, Airway Biology, and Nasal Disorders in AJRCCM 2001
Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 598 - 618.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2001 American Thoracic Society