Am. J. Respir. Crit. Care Med., Volume 164, Number 10, November 2001, 1957-1963

Exposure to Diesel Exhaust Exacerbates Allergen-induced Airway Responses in Guinea Pigs

KEN-ICHI HASHIMOTO, YUKIO ISHII, YOSHIYUKI UCHIDA, TORU KIMURA, KUNIKO MASUYAMA, YUKO MORISHIMA, KUNIYOSHI HIRANO, AKIHIRO NOMURA, TOHRU SAKAMOTO, HIROHISA TAKANO, MASARU SAGAI, and KIYOHISA SEKIZAWA

Department of Respiratory Medicine, University of Tsukuba, and National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan

Diesel exhaust (DE) is a major air pollutant in urban areas. To clarify the effects of DE on the exacerbation of asthma, guinea pigs were exposed 12 h daily to 3 mg/m³ DE or air for 8 wk with or without sensitization to ovalbumin (OVA). In the DE-exposed sensitized animals, both immediate (IAR) and late (LAR) airway responses were enhanced after the inhalation challenge by OVA, compared with the DE-unexposed sensitized animals. Mucus was greatly accumulated in the airways of DE-exposed sensitized animals during IAR. The number of eosinophils and level of sialic acid concentration in bronchial lavage fluids were also significantly higher in the DE-exposed sensitized animals than in the DE-unexposed control animals. During LAR, intercellular spaces of the bronchial epithelium became enlarged in the DE-exposed sensitized animals, showing infiltration by numerous eosinophils. Albumin concentration was significantly higher in the bronchial lavage fluids from the DE-exposed sensitized animals than in those from the DE-unexposed control animals. These results suggest that exposure to DE enhances mucus hypersecretion and eosinophilic inflammation during IAR. DE exposure also increases airway permeability and airway inflammation during LAR. Thus, DE exposure exacerbates allergen-induced airway responses in guinea pigs.

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