help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by TAKIZAWA, H.
Right arrow Articles by UMEDA, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by TAKIZAWA, H.
Right arrow Articles by UMEDA, A.

Am. J. Respir. Crit. Care Med., Volume 163, Number 6, May 2001, 1476-1483

Increased Expression of Transforming Growth Factor-beta 1 in Small Airway Epithelium from Tobacco Smokers and Patients with Chronic Obstructive Pulmonary Disease (COPD)

HAJIME TAKIZAWA, MITSURU TANAKA, KAZUTAKA TAKAMI, TAKAYUKI OHTOSHI, KOJI ITO, MASARU SATOH, YASUMASA OKADA, FUMIHIRO YAMASAWA, KAZUHIKO NAKAHARA, and AKIRA UMEDA

Department of Laboratory Medicine and Respiratory Medicine, Tokyo University, School of Medicine, Tokyo, Japan; WHO Collaborating Centre, Tokyo Medical College, Tokyo, Japan; and Department of Diagnostic Radiology, Keio University, School of Medicine, Tokyo, Japan

Tobacco smoke is believed to cause small airway disease and then chronic obstructive pulmonary disease (COPD), but the molecular mechanisms by which small airway obstruction occurs remain unknown. To study the gene expression levels of transforming growth factor (TGF)-beta 1, a potent fibrogenic factor, in small airway epithelium from smokers and patients with COPD, we harvested highly pure samples of epithelial cells from small airways under direct vision by using an ultrathin bronchofiberscope BF-2.7T (outer diameter 2.7 mm with a biopsy channel of 0.8 mm in diameter). The expression levels of TGF-beta 1 were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The mRNA levels of TGF-beta 1 corrected by beta -actin transcripts were significantly higher in the smoking group and patients with COPD than those in nonsmokers (p < 0.01). Furthermore, among smokers and patients with COPD, TGF-beta 1 mRNA levels correlated positively with the extent of smoking history (pack-years) and the degree of small airway obstruction as assessed by measurements of flow-volume curves. Immunocytochemistry of the cells demonstrated more intense stainings for TGF-beta 1 in samples from smokers and patients with COPD than from nonsmokers. Spontaneously released immunoreactive TGF-beta 1 levels from cultured epithelial cells were more elevated in subjects with a history of smoking and patients with COPD than in nonsmokers. Our study showed a close link between smoking and expression of TGF-beta 1 in small airways. Our results also suggested that small airway epithelial cells might be involved in obstructive changes found in smokers and patients with COPD.




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. J. Barnes
The Cytokine Network in Chronic Obstructive Pulmonary Disease
Am. J. Respir. Cell Mol. Biol., December 1, 2009; 41(6): 631 - 638.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
K. F. Chung and I. M. Adcock
Multifaceted mechanisms in COPD: inflammation, immunity, and tissue repair and destruction
Eur. Respir. J., June 1, 2008; 31(6): 1334 - 1356.
[Abstract] [Full Text] [PDF]

Home page
 Ther Adv Respir DisHome page
A. Zanini, A. Chetta, and D. Olivieri
Review: Therapeutic perspectives in bronchial vascular remodeling in COPD
Therapeutic Advances in Respiratory Disease, June 1, 2008; 2(3): 179 - 187.
[Abstract] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Yamasaki, H.-R. Kang, R. J. Homer, S. P. Chapoval, S. J. Cho, B. J. Lee, J. A. Elias, and C. G. Lee
P21 Regulates TGF-{beta}1-Induced Pulmonary Responses via a TNF-{alpha}-Signaling Pathway
Am. J. Respir. Cell Mol. Biol., March 1, 2008; 38(3): 346 - 353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
I-M. Wang, S. Stepaniants, Y. Boie, J. R. Mortimer, B. Kennedy, M. Elliott, S. Hayashi, L. Loy, S. Coulter, S. Cervino, et al.
Gene Expression Profiling in Patients with Chronic Obstructive Pulmonary Disease and Lung Cancer
Am. J. Respir. Crit. Care Med., February 15, 2008; 177(4): 402 - 411.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Liu, S.-C. Yang, S. Sharma, J. Luo, X. Cui, K. A. Peebles, M. Huang, M. Sato, R. D. Ramirez, J. W. Shay, et al.
EGFR Signaling Is Required for TGF-beta1 Mediated COX-2 Induction in Human Bronchial Epithelial Cells
Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 578 - 588.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Elizur, T. L. Adair-Kirk, D. G. Kelley, G. L. Griffin, D. E. deMello, and R. M. Senior
Clara cells impact the pulmonary innate immune response to LPS
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L383 - L392.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Sturrock, T. P. Huecksteadt, K. Norman, K. Sanders, T. M. Murphy, P. Chitano, K. Wilson, J. R. Hoidal, and T. P. Kennedy
Nox4 mediates TGF-beta1-induced retinoblastoma protein phosphorylation, proliferation, and hypertrophy in human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1543 - L1555.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Maunders, S. Patwardhan, J. Phillips, A. Clack, and A. Richter
Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro
Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1248 - L1256.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Churg, H. Tai, T. Coulthard, R. Wang, and J. L. Wright
Cigarette Smoke Drives Small Airway Remodeling by Induction of Growth Factors in the Airway Wall
Am. J. Respir. Crit. Care Med., December 15, 2006; 174(12): 1327 - 1334.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Zandvoort, D. S. Postma, M. R. Jonker, J. A. Noordhoek, J. T. W. M. Vos, Y. M. van der Geld, and W. Timens
Altered expression of the Smad signalling pathway: implications for COPD pathogenesis
Eur. Respir. J., September 1, 2006; 28(3): 533 - 541.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
B. Beghe, E. Bazzan, S. Baraldo, F. Calabrese, F. Rea, M. Loy, P. Maestrelli, R. Zuin, L. M. Fabbri, and M. Saetta
Transforming growth factor-{beta} type II receptor in pulmonary arteries of patients with very severe COPD
Eur. Respir. J., September 1, 2006; 28(3): 556 - 562.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
D. S. Postma and W. Timens
Remodeling in asthma and chronic obstructive pulmonary disease.
Proceedings of the ATS, July 1, 2006; 3(5): 434 - 439.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K.-Y. Lee, K. Ito, R. Hayashi, E. P. I. Jazrawi, P. J. Barnes, and I. M. Adcock
NF-{kappa}B and Activator Protein 1 Response Elements and the Role of Histone Modifications in IL-1{beta}-Induced TGF-{beta}1 Gene Transcription
J. Immunol., January 1, 2006; 176(1): 603 - 615.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
S Baraldo, E Bazzan, G Turato, F Calabrese, B Beghe, A Papi, P Maestrelli, L M Fabbri, R Zuin, and M Saetta
Decreased expression of TGF-{beta} type II receptor in bronchial glands of smokers with COPD
Thorax, December 1, 2005; 60(12): 998 - 1002.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
W. MacNee
Pathogenesis of Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2005; 2(4): 258 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
K. F. Chung
The Role of Airway Smooth Muscle in the Pathogenesis of Airway Wall Remodeling in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2005; 2(4): 347 - 354.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. D. Wang, J. L. Wright, and A. Churg
Transforming Growth Factor-{beta}1 Drives Airway Remodeling in Cigarette Smoke-Exposed Tracheal Explants
Am. J. Respir. Cell Mol. Biol., October 1, 2005; 33(4): 387 - 393.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
P. J. Barnes
New approaches to COPD
Eur. Respir. Rev., September 1, 2005; 14(94): 2 - 11.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P. J. Barnes and R. A. Stockley
COPD: current therapeutic interventions and future approaches
Eur. Respir. J., June 1, 2005; 25(6): 1084 - 1106.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
A. Baydur
Not All That Comes Out Is Hot Air
Chest, May 1, 2005; 127(5): 1482 - 1485.
[Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. F. M. Wouters
Local and Systemic Inflammation in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, April 1, 2005; 2(1): 26 - 33.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
W. MacNee
Pulmonary and Systemic Oxidant/Antioxidant Imbalance in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, April 1, 2005; 2(1): 50 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Izzotti, C. Cartiglia, M. Longobardi, M. Bagnasco, A. Merello, M. You, R. A. Lubet, and S. De Flora
Gene Expression in the Lung of p53 Mutant Mice Exposed to Cigarette Smoke
Cancer Res., December 1, 2004; 64(23): 8566 - 8572.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
P. J. Barnes
Mediators of Chronic Obstructive Pulmonary Disease
Pharmacol. Rev., December 1, 2004; 56(4): 515 - 548.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Ning, C.-J. Li, N. Kaminski, C. A. Feghali-Bostwick, S. M. Alber, Y. P. Di, S. L. Otterbein, R. Song, S. Hayashi, Z. Zhou, et al.
Comprehensive gene expression profiles reveal pathways related to the pathogenesis of chronic obstructive pulmonary disease
PNAS, October 12, 2004; 101(41): 14895 - 14900.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. G. Lee, S. J. Cho, M. J. Kang, S. P. Chapoval, P. J. Lee, P. W. Noble, T. Yehualaeshet, B. Lu, R. A. Flavell, J. Milbrandt, et al.
Early Growth Response Gene 1-mediated Apoptosis Is Essential for Transforming Growth Factor {beta}1-induced Pulmonary Fibrosis
J. Exp. Med., August 2, 2004; 200(3): 377 - 389.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. Elias
The Relationship Between Asthma and COPD: Lessons From Transgenic Mice
Chest, August 1, 2004; 126(2_suppl_1): 111S - 116S.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P.J. Barnes, S.D. Shapiro, and R.A. Pauwels
Chronic obstructive pulmonary disease: molecular and cellularmechanisms
Eur. Respir. J., October 1, 2003; 22(4): 672 - 688.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Cisneros-Lira, M. Gaxiola, C. Ramos, M. Selman, and A. Pardo
Cigarette smoke exposure potentiates bleomycin-induced lung fibrosis in guinea pigs
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L949 - L956.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. Schulz, V. Petrig, K. Wolf, K. Kratzel, M. Kohler, B. Becker, and M. Pfeifer
Upregulation of MCAM in primary bronchial epithelial cells from patients with COPD
Eur. Respir. J., September 1, 2003; 22(3): 450 - 456.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
I. Rahman, A. A. M. van Schadewijk, A. J. L. Crowther, P. S. Hiemstra, J. Stolk, W. MacNee, and W. I. De Boer
4-Hydroxy-2-Nonenal, a Specific Lipid Peroxidation Product, Is Elevated in Lungs of Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., August 15, 2002; 166(4): 490 - 495.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Y. Higashimoto, W. M. Elliott, A. R. Behzad, E. G. Sedgwick, T. Takei, J. C. Hogg, and S. Hayashi
Inflammatory Mediator mRNA Expression by Adenovirus E1A-Transfected Bronchial Epithelial Cells
Am. J. Respir. Crit. Care Med., July 15, 2002; 166(2): 200 - 207.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Jardine, W. MacNee, K. Donaldson, and I. Rahman
Molecular Mechanism of Transforming Growth Factor (TGF)-beta 1-induced Glutathione Depletion in Alveolar Epithelial Cells. INVOLVEMENT OF AP-1/ARE AND Fra-1
J. Biol. Chem., June 7, 2002; 277(24): 21158 - 21166.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Chronic Obstructive Pulmonary Disease, Pollution, Pulmonary Vascular Disease, Transplantation, Pleural Disease, and Lung Cancer in AJRCCM 2001
Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 642 - 662.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2001 American Thoracic Society 		  ATS Coding and Billing Quarterly