help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WEICKER, S.
Right arrow Articles by MEHTA, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WEICKER, S.
Right arrow Articles by MEHTA, S.

Am. J. Respir. Crit. Care Med., Volume 163, Number 5, April 2001, 1113-1116

Noninvasive Measurement of Exhaled Nitric Oxide in a Spontaneously Breathing Mouse

SEAN WEICKER, TIMOTHY A. KARACHI, JEREMY A. SCOTT, DAVE G. McCORMACK, and SANJAY MEHTA

A.C. Burton Vascular Biology Laboratory, London Health Science Centre, Department of Pharmacology and Toxicology, and Division of Respirology, Department of Medicine, University of Western Ontario, London, Ontario, Canada

Nitric oxide (NO) has been detected in the exhaled gas of animals and humans. In previous work, investigators have used anesthetized, mechanically ventilated animals to obtain exhaled NO (ENO) measurements, which has unclear effects on the levels of ENO and does not allow for repeated analysis of ENO. We sought to measure ENO from a single, spontaneously breathing mouse. The mouse was placed in a small Plexiglas chamber and allowed to acclimatize before exhaled gas was collected for ENO analysis. Under optimal operating conditions of flow and pressure, the mean concentration of exhaled NO (FENO) of 25 mice was 10.1 ± 1.0 ppb. The maximal variation of FENO when repeatedly measured daily in individual animals was 2.1 ppb. Administration of L-NAME, a nonselective NOS inhibitor, reduced FENO by 51 ± 6% (p < 0.01). Intraperitoneally administered lipopolysaccharide induced acute lung injury and increased FENO by 30 ± 7% (p < 0.05). We have demonstrated that it is possible to noninvasively measure ENO from a single, spontaneously breathing mouse. This novel technique provides a stable, reproducible, and responsive measure of ENO in mice. This technique will be of use in determining cellular and isoform sources of ENO, as well as the role of endogenous NO in lung disease.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
B. T. Ameredes, R. Zamora, J. M. Sethi, H.-L. Liu, L. K. Kohut, A. L. Gligonic, A. M. K. Choi, and W. J. Calhoun
Alterations in Nitric Oxide and Cytokine Production with Airway Inflammation in the Absence of IL-10
J. Immunol., July 15, 2005; 175(2): 1206 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. T. Ameredes, J. M. Sethi, H.-L. Liu, A. M. K. Choi, and W. J. Calhoun
Enhanced nitric oxide production associated with airway hyporesponsiveness in the absence of IL-10
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L868 - L873.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. McCluskie, M. A. Birrell, S. Wong, and M. G. Belvisi
Nitric Oxide as a Noninvasive Biomarker of Lipopolysaccharide-Induced Airway Inflammation: Possible Role in Lung Neutrophilia
J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 625 - 633.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Hjoberg, S. Shore, L. Kobzik, S. Okinaga, A. Hallock, J. Vallone, V. Subramaniam, G. T. De Sanctis, J. A. Elias, J. M. Drazen, et al.
Expression of nitric oxide synthase-2 in the lungs decreases airway resistance and responsiveness
J Appl Physiol, July 1, 2004; 97(1): 249 - 259.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
A. M. Dukelow, S. Weicker, T. A. Karachi, H. M. Razavi, D. G. McCormack, M. G. Joseph, and S. Mehta
Effects of Nebulized Diethylenetetraamine-NONOate in a Mouse Model of Acute Pseudomonas aeruginosa Pneumonia
Chest, December 1, 2002; 122(6): 2127 - 2136.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Asthma, Airway Biology, and Nasal Disorders in AJRCCM 2001
Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 598 - 618.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2001 American Thoracic Society