help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by KITAMURA, Y.
Right arrow Articles by NAKAJIMA, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by KITAMURA, Y.
Right arrow Articles by NAKAJIMA, H.

Am. J. Respir. Crit. Care Med., Volume 163, Number 3, March 2001, 762-769

Fas/FasL-dependent Apoptosis of Alveolar Cells after Lipopolysaccharide-induced Lung Injury in Mice

YOSHIHIRO KITAMURA, SATORU HASHIMOTO, NARUHIKO MIZUTA, ATSUKO KOBAYASHI, KUNIHIKO KOOGUCHI, IKUYA FUJIWARA, and HIROO NAKAJIMA

Department of Intensive Care and Anesthesiology and Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan

To determine the possible contribution of apoptosis in the pathogenesis of acute lung injury (ALI), we investigated Fas antigen (Fas), Fas ligand (FasL), perforin, granzyme A, and granzyme B expressions in a murine model of ALI after intratracheal instillation of Escherichia coli lipopolysaccharide (LPS: 0.3-30 µg) into the left lung. Lung injury, examined by water-to-dry weight ratio and albumin leakage, demonstrated maximal epithelial injury 1 d after 30 µg LPS instillation. Expressions of the proapoptosis molecules' mRNA were dose-dependently up-regulated, with maximal expression in the early phase in the instilled lung and most apparent 1 d after LPS instillation. Negligible mRNA expression of proapoptosis molecules was observed in noninstilled lungs. The terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) demonstrated positive signals in neutrophils and macrophages as well as in alveolar wall cells of the instilled lung 1 d after LPS instillation. Immunohistochemistry demonstrated that Fas was up-regulated in alveolar and inflammatory cells and FasL-positive inflammatory cells migrated into the air spaces in the LPS-instilled lung. Intratracheal administration of P2 antibody, which is an anti-Fas blocking antibody, attenuated the lung injury after 30 µg LPS instillation without attenuating mRNA expressions of proapoptosis molecules and neutrophil accumulation in the lung. In contrast, concanamycin A, which inhibits the function of perforin, did not alter the outcome after LPS instillation. These results indicate that the Fas/FasL system could be important in the pathogenesis of LPS-induced ALI, and proper regulation of the FasL/Fas system might be important for potential treatment of ARDS.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
H. T. Hassoun, M. L. Lie, D. N. Grigoryev, M. Liu, R. M. Tuder, and H. Rabb
Kidney ischemia-reperfusion injury induces caspase-dependent pulmonary apoptosis
Am J Physiol Renal Physiol, July 1, 2009; 297(1): F125 - F137.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
R. MacRedmond, G. K. Singhera, and D. R. Dorscheid
Erythropoietin inhibits respiratory epithelial cell apoptosis in a model of acute lung injury
Eur. Respir. J., June 1, 2009; 33(6): 1403 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-C. Hou, M.-H. Pai, W.-C. Chiu, Y.-M. Hu, and S.-L. Yeh
Effects of dietary glutamine supplementation on lung injury induced by lipopolysaccharide administration
Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L288 - L295.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Y. P. Bhandary, T. Velusamy, P. Shetty, R. S. Shetty, S. Idell, D. B. Cines, D. Jain, K. Bdeir, E. Abraham, Y. Tsuruta, et al.
Post-Transcriptional Regulation of Urokinase-type Plasminogen Activator Receptor Expression in Lipopolysaccharide-induced Acute Lung Injury
Am. J. Respir. Crit. Care Med., February 15, 2009; 179(4): 288 - 298.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Herold, M. Steinmueller, W. von Wulffen, L. Cakarova, R. Pinto, S. Pleschka, M. Mack, W. A. Kuziel, N. Corazza, T. Brunner, et al.
Lung epithelial apoptosis in influenza virus pneumonia: the role of macrophage-expressed TNF-related apoptosis-inducing ligand
J. Exp. Med., December 22, 2008; 205(13): 3065 - 3077.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Q. Mao, S. Gundavarapu, C. Patel, A. Tsai, F. I. Luks, and M. E. De Paepe
The Fas System Confers Protection against Alveolar Disruption in Hyperoxia-Exposed Newborn Mice
Am. J. Respir. Cell Mol. Biol., December 1, 2008; 39(6): 717 - 729.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Hashimoto, F. Amaya, H. Matsuyama, H. Ueno, S. Kikuchi, M. Tanaka, Y. Watanabe, M. Ebina, A. Ishizaka, S. Tsukita, et al.
Dysregulation of lung injury and repair in moesin-deficient mice treated with intratracheal bleomycin
Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L566 - L574.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. Zhang, S. Tasaka, Y. Shiraishi, K. Fukunaga, W. Yamada, H. Seki, Y. Ogawa, K. Miyamoto, Y. Nakano, N. Hasegawa, et al.
Role of Soluble Receptor for Advanced Glycation End Products on Endotoxin-induced Lung Injury
Am. J. Respir. Crit. Care Med., August 15, 2008; 178(4): 356 - 362.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. E. De Paepe, S. Gundavarapu, U. Tantravahi, J. R. Pepperell, S. A. Haley, F. I. Luks, and Q. Mao
Fas-Ligand-Induced Apoptosis of Respiratory Epithelial Cells Causes Disruption of Postcanalicular Alveolar Development
Am. J. Pathol., July 1, 2008; 173(1): 42 - 56.
[Abstract] [Full Text] [PDF]

Home page
 Lab AnimHome page
J Hamacher, M Arras, F Bootz, M Weiss, R Schramm, and U Moehrlen
Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination
Lab Anim, April 1, 2008; 42(2): 222 - 230.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. S. Tang, M. Mura, R. Seth, and M. Liu
Acute lung injury and cell death: how many ways can cells die?
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L632 - L641.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. Glassroth
Pulmonary Disease Due to Nontuberculous Mycobacteria
Chest, January 1, 2008; 133(1): 243 - 251.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
G. M. Mutlu, D. Machado-Aranda, J. E. Norton, A. Bellmeyer, D. Urich, R. Zhou, and D. A. Dean
Electroporation-mediated Gene Transfer of the Na+,K+-ATPase Rescues Endotoxin-induced Lung Injury
Am. J. Respir. Crit. Care Med., September 15, 2007; 176(6): 582 - 590.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. Perl, C.-S. Chung, U. Perl, J. Lomas-Neira, M. de Paepe, W. G. Cioffi, and A. Ayala
Fas-induced Pulmonary Apoptosis and Inflammation during Indirect Acute Lung Injury
Am. J. Respir. Crit. Care Med., September 15, 2007; 176(6): 591 - 601.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. L. Wang, I. O. Akinci, C. M. Baker, D. Urich, A. Bellmeyer, M. Jain, N. S. Chandel, G. M. Mutlu, and G. R. S. Budinger
The Intrinsic Apoptotic Pathway Is Required for Lipopolysaccharide-Induced Lung Endothelial Cell Death
J. Immunol., August 1, 2007; 179(3): 1834 - 1841.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W.-F. Fang, J. H. Cho, Q. He, M.-C. Lin, C.-C. Wu, N. F. Voelkel, and I. S. Douglas
Lipid A fraction of LPS induces a discrete MAPK activation in acute lung injury
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L336 - L344.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Itoh, H. Obata, S. Murakami, K. Hamada, K. Kangawa, H. Kimura, and N. Nagaya
Adrenomedullin ameliorates lipopolysaccharide-induced acute lung injury in rats
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L446 - L452.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Cuzzocrea, G. Nocentini, R. Di Paola, M. Agostini, E. Mazzon, S. Ronchetti, C. Crisafulli, E. Esposito, A. P. Caputi, and C. Riccardi
Proinflammatory Role of Glucocorticoid-Induced TNF Receptor-Related Gene in Acute Lung Inflammation
J. Immunol., July 1, 2006; 177(1): 631 - 641.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
D. Shitrit, G. L. Baum, R. Priess, A. Lavy, A. B.-G. Shitrit, M. Raz, D. Shlomi, B. Daniele, and M. R. Kramer
Pulmonary Mycobacterium kansasii Infection in Israel, 1999-2004: Clinical Features, Drug Susceptibility, and Outcome
Chest, March 1, 2006; 129(3): 771 - 776.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Perl, C.-S. Chung, J. Lomas-Neira, T.-M. Rachel, W. L. Biffl, W. G. Cioffi, and A. Ayala
Silencing of Fas, but Not Caspase-8, in Lung Epithelial Cells Ameliorates Pulmonary Apoptosis, Inflammation, and Neutrophil Influx after Hemorrhagic Shock and Sepsis
Am. J. Pathol., December 1, 2005; 167(6): 1545 - 1559.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. E. De Paepe, Q. Mao, Y. Chao, J. L. Powell, L. P. Rubin, and S. Sharma
Hyperoxia-induced apoptosis and Fas/FasL expression in lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L647 - L659.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Matute-Bello, J. S. Lee, W. C. Liles, C. W. Frevert, S. Mongovin, V. Wong, K. Ballman, S. Sutlief, and T. R. Martin
Fas-Mediated Acute Lung Injury Requires Fas Expression on Nonmyeloid Cells of the Lung
J. Immunol., September 15, 2005; 175(6): 4069 - 4075.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
D. E. Wesche, J. L. Lomas-Neira, M. Perl, C.-S. Chung, and A. Ayala
Leukocyte apoptosis and its significance in sepsis and shock
J. Leukoc. Biol., August 1, 2005; 78(2): 325 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Jeyaseelan, H. W. Chu, S. K. Young, M. W. Freeman, and G. S. Worthen
Distinct Roles of Pattern Recognition Receptors CD14 and Toll-Like Receptor 4 in Acute Lung Injury
Infect. Immun., March 1, 2005; 73(3): 1754 - 1763.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. A. Neff, R.-F. Guo, S. B. Neff, J. V. Sarma, C. L. Speyer, H. Gao, K. D. Bernacki, M. Huber-Lang, S. McGuire, L. M. Hoesel, et al.
Relationship of Acute Lung Inflammatory Injury to Fas/FasL System
Am. J. Pathol., March 1, 2005; 166(3): 685 - 694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. D. Douillet, W. P. Robinson III, B. L. Zarzaur, E. R. Lazarowski, R. C. Boucher, and P. B. Rich
Mechanical Ventilation Alters Airway Nucleotides and Purinoceptors in Lung and Extrapulmonary Organs
Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 52 - 58.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Bao, Y. Wang, P. Sweeney, A. Chaudhuri, A. I. Doseff, C. B. Marsh, and D. L. Knoell
Keratinocyte growth factor induces Akt kinase activity and inhibits Fas-mediated apoptosis in A549 lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L36 - L42.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Jeyaseelan, H. W. Chu, S. K. Young, and G. S. Worthen
Transcriptional Profiling of Lipopolysaccharide-Induced Acute Lung Injury
Infect. Immun., December 1, 2004; 72(12): 7247 - 7256.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. Nakagomi, O. Kitada, K. Kuribayashi, H. Yoshikawa, K. Ozawa, S. Ogawa, and T. Matsuyama
The 150-Kilodalton Oxygen-Regulated Protein Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice
Am. J. Pathol., October 1, 2004; 165(4): 1279 - 1288.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
X. Peng, P. M. Hassoun, S. Sammani, B. J. McVerry, M. J. Burne, H. Rabb, D. Pearse, R. M. Tuder, and J. G. N. Garcia
Protective Effects of Sphingosine 1-Phosphate in Murine Endotoxin-induced Inflammatory Lung Injury
Am. J. Respir. Crit. Care Med., June 1, 2004; 169(11): 1245 - 1251.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
G. Matute-Bello, R. K. Winn, T. R. Martin, and W. C. Liles
Sustained Lipopolysaccharide-Induced Lung Inflammation in Mice Is Attenuated by Functional Deficiency of the Fas/Fas Ligand System
Clin. Vaccine Immunol., March 1, 2004; 11(2): 358 - 361.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. C. Parker and M. I. Townsley
Evaluation of lung injury in rats and mice
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L231 - L246.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Yamada, H. Kubo, S. Kobayashi, K. Ishizawa, M. Numasaki, S. Ueda, T. Suzuki, and H. Sasaki
Bone Marrow-Derived Progenitor Cells Are Important for Lung Repair after Lipopolysaccharide-Induced Lung Injury
J. Immunol., January 15, 2004; 172(2): 1266 - 1272.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhang, P. Shan, J. Alam, R. J. Davis, R. A. Flavell, and P. J. Lee
Carbon Monoxide Modulates Fas/Fas Ligand, Caspases, and Bcl-2 Family Proteins via the p38{alpha} Mitogen-activated Protein Kinase Pathway during Ischemia-Reperfusion Lung Injury
J. Biol. Chem., June 6, 2003; 278(24): 22061 - 22070.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Ayala, C.-S. Chung, J. L. Lomas, G. Y. Song, L. A. Doughty, S. H. Gregory, W. G. Cioffi, B. W. LeBlanc, J. Reichner, H. H. Simms, et al.
Shock-Induced Neutrophil Mediated Priming for Acute Lung Injury in Mice: Divergent Effects of TLR-4 and TLR-4/FasL Deficiency
Am. J. Pathol., December 1, 2002; 161(6): 2283 - 2294.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. H. Albertine, M. F. Soulier, Z. Wang, A. Ishizaka, S. Hashimoto, G. A. Zimmerman, M. A. Matthay, and L. B. Ware
Fas and Fas Ligand Are Up-Regulated in Pulmonary Edema Fluid and Lung Tissue of Patients with Acute Lung Injury and the Acute Respiratory Distress Syndrome
Am. J. Pathol., November 1, 2002; 161(5): 1783 - 1796.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. De Carlo Massaro, S. Radaeva, L. B. Clerch, and D. Massaro
Lung alveoli: endogenous programmed destruction and regeneration
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L305 - L309.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M.-C. Plotkowski, H. C. C. Povoa, J.-M. Zahm, G. Lizard, G. M. B. Pereira, J.-M. Tournier, and E. Puchelle
Early Mitochondrial Dysfunction, Superoxide Anion Production, and DNA Degradation Are Associated with Non-Apoptotic Death of Human Airway Epithelial Cells Induced by Pseudomonas aeruginosa Exotoxin A
Am. J. Respir. Cell Mol. Biol., May 1, 2002; 26(5): 617 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Critical Care Medicine in AJRCCM 2001
Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 565 - 583.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. M. EDWARDS, M. H. CYNAMON, R. K. R. VOLADRI, C. C. HAGER, M. S. DESTEFANO, K. T. THAM, D. L. LAKEY, M. R. BOCHAN, and D. S. KERNODLE
Iron-cofactored Superoxide Dismutase Inhibits Host Responses to Mycobacterium tuberculosis
Am. J. Respir. Crit. Care Med., December 15, 2001; 164(12): 2213 - 2219.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. S. Hotchkiss, W. M. Dunne, P. E. Swanson, C. G. Davis, K. W. Tinsley, K. C. Chang, T. G. Buchman, I. E. Karl, H. Grassme, S. Kirschnek, et al.
Role of Apoptosis in Pseudomonas aeruginosa Pneumonia
Science, November 30, 2001; 294(5548): 1783a - 1783.
[Full Text] [PDF]

Home page
 JEMHome page
V. M. Borges, H. Falcao, J. H. Leite-Junior, L. Alvim, G. P. Teixeira, M. Russo, A. F. Nobrega, M. F. Lopes, P. M. Rocco, W. F. Davidson, et al.
Fas Ligand Triggers Pulmonary Silicosis
J. Exp. Med., July 16, 2001; 194(2): 155 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. M. Konstadoulakis, E. G. Messaris, S. Hashimoto, A. Kobayashi, and H. Nakajima
ARE CANCER PATIENTS ADEQUATE CANDIDATES FOR STUDYING APOPTOSIS IN SEPTIC AND ARDS MODELS?
Am. J. Respir. Crit. Care Med., May 1, 2001; 163(6): 1500 - 1500.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2001 American Thoracic Society 		  ATS Par News