help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WAHLSTRÖM, J.
Right arrow Articles by GRUNEWALD, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WAHLSTRÖM, J.
Right arrow Articles by GRUNEWALD, J.

Am. J. Respir. Crit. Care Med., Volume 163, Number 1, January 2001, 115-121

Analysis of Intracellular Cytokines in CD4+ and CD8+ Lung and Blood T Cells in Sarcoidosis

JAN WAHLSTRÖM, KIANOOSH KATCHAR, HANS WIGZELL, OLLE OLERUP, ANDERS EKLUND, and JOHAN GRUNEWALD

Microbiology and Tumour Biology Centre, Karolinska Institutet, and Department of Medicine, Karolinska Hospital, Stockholm; and Center for BioTechnology, Karolinska Institutet, NOVUM, Huddinge, Sweden

In pulmonary sarcoidosis, activated T cells accumulate in the lungs. We hypothesized that the balance between the T-helper type 1 (Th1) cytokines (interferon [IFN]-gamma and interleukin [IL]-2) and Th2 cytokines such as IL-4, IL-5, and IL-10 might explain differences in clinical outcome in pulmonary sarcoidosis, such as why patients of human leukocyte antigen (HLA) type DR17 have a much better prognosis than those of other HLA types. Peripheral blood lymphocytes (PBL) and lymphocytes obtained by bronchoalveolar lavage (BAL) from HLA-typed sarcoidosis patients, as well as PBL from healthy controls, were stimulated in vitro, fixed, and permeabilized with saponin. Thereafter, cells were stained with fluorescence- labeled antibodies specific for intracellular cytokines (IL-2, IL-4, IFN-gamma , and tumor necrosis factor (TNF)-alpha and cell surface markers CD4 and CD8, and were subjected to flow-cytometric analysis. In bronchoalveolar lavage fluid (BALF), there were significantly greater frequencies of T cells positive for IFN-gamma and TNF-alpha than there were among PBL, and significantly fewer cells positive for IL-4, in both the CD4+ and CD8+ subsets. HLA-DR17-positive patients showed a tendency toward a less pronounced Th1 response that may be related to their good prognosis. Sarcoidosis patients had higher frequencies of cells positive for IFN-gamma , IL-4, and IL-2 in their blood than did healthy controls, a finding that may reflect the systemic nature of sarcoidosis. A clear Th1 cytokine profile of CD4+ as well as of CD8+ T cells was demonstrated in BALF from sarcoidosis patients. This was most pronounced for CD8+ cells, which may therefore make an important contribution to the inflammatory process in the lungs in pulmonary sarcoidosis.




This article has been cited by other articles:


Home page
 Eur Respir JHome page
A. Busuttil, S. S. Weigt, M. P. Keane, Y. Y. Xue, V. Palchevskiy, M. D. Burdick, C. Huang, D. A. Zisman, M. Fishbein, J. P. Lynch III, et al.
CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis
Eur. Respir. J., September 1, 2009; 34(3): 676 - 686.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Mathew, K. L. Bauer, A. Fischoeder, N. Bhardwaj, and S. J. Oliver
The Anergic State in Sarcoidosis Is Associated with Diminished Dendritic Cell Function
J. Immunol., July 1, 2008; 181(1): 746 - 755.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
M. C. Iannuzzi, B. A. Rybicki, and A. S. Teirstein
Sarcoidosis
N. Engl. J. Med., November 22, 2007; 357(21): 2153 - 2165.
[Full Text] [PDF]

Home page
 Clin Med ResHome page
K. G.-J. Ooi, G. Galatowicz, V. L. Calder, and S. L. Lightman
Cytokines and Chemokines in Uveitis - Is there a Correlation with Clinical Phenotype?
Clin. Med. Res., December 1, 2006; 4(4): 294 - 309.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
K. M. Antoniou, I. Tsiligianni, D. Kyriakou, N. Tzanakis, A. Tzouvelekis, N. M. Siafakas, and D. Bouros
Perforin Down-Regulation and Adhesion Molecules Activation in Pulmonary Sarcoidosis: An Induced Sputum and BAL Study
Chest, June 1, 2006; 129(6): 1592 - 1598.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
O. Akbari, J. L. Faul, E. G. Hoyte, G. J. Berry, J. Wahlstrom, M. Kronenberg, R. H. DeKruyff, and D. T. Umetsu
CD4+ invariant T-cell-receptor+ natural killer T cells in bronchial asthma.
N. Engl. J. Med., March 16, 2006; 354(11): 1117 - 1129.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
F. Idali, M. Wiken, J. Wahlstrom, H. Mellstedt, A. Eklund, H. Rabbani, and J. Grunewald
Reduced Th1 response in the lungs of HLA-DRB1*0301 patients with pulmonary sarcoidosis.
Eur. Respir. J., March 1, 2006; 27(3): 451 - 459.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. T. Sawyer, C. E. Parsons, A. P. Fontenot, L. A. Maier, M. M. Gillespie, E. B. Gottschall, L. Silveira, and L. S. Newman
Beryllium-Induced Tumor Necrosis Factor-{alpha} Production by CD4+ T Cells Is Mediated by HLA-DP
Am. J. Respir. Cell Mol. Biol., July 1, 2004; 31(1): 122 - 130.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
M Ota, R Amakawa, K Uehira, T Ito, Y Yagi, A Oshiro, Y Date, H Oyaizu, T Shigeki, Y Ozaki, et al.
Involvement of dendritic cells in sarcoidosis
Thorax, May 1, 2004; 59(5): 408 - 413.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. Grunewald, A. Eklund, and O. Olerup
Human Leukocyte Antigen Class I Alleles and the Disease Course in Sarcoidosis Patients
Am. J. Respir. Crit. Care Med., March 15, 2004; 169(6): 696 - 702.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. A. Maier, D. S. McGrath, H. Sato, P. Lympany, K. Welsh, R. du Bois, L. Silveira, A. P. Fontenot, R. T. Sawyer, E. Wilcox, et al.
Influence of MHC CLASS II in Susceptibility to Beryllium Sensitization and Chronic Beryllium Disease
J. Immunol., December 15, 2003; 171(12): 6910 - 6918.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
H. Fehrenbach, G. Zissel, T. Goldmann, T. Tschernig, E. Vollmer, R. Pabst, and J. Muller-Quernheim
Alveolar macrophages are the main source for tumour necrosis factor-{alpha} in patients with sarcoidosis
Eur. Respir. J., March 1, 2003; 21(3): 421 - 428.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
S. A. Hines, D. M. Stone, M. T. Hines, D. C. Alperin, D. P. Knowles, L. K. Norton, M. J. Hamilton, W. C. Davis, and T. C. McGuire
Clearance of Virulent but Not Avirulent Rhodococcus equi from the Lungs of Adult Horses Is Associated with Intracytoplasmic Gamma Interferon Production by CD4+ and CD8+ T Lymphocytes
Clin. Vaccine Immunol., March 1, 2003; 10(2): 208 - 215.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. R. Moller and E. S. Chen
Genetic Basis of Remitting Sarcoidosis: Triumph of the Trimolecular Complex?
Am. J. Respir. Cell Mol. Biol., October 1, 2002; 27(4): 391 - 395.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Tuberculosis, Lung Infections, Interstitial Lung Disease, and Socioeconomic Issues in AJRCCM 2001
Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 631 - 641.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2001 American Thoracic Society 		  CCM abstracts