help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BLACKWELL, T. S.
Right arrow Articles by KERR, L. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BLACKWELL, T. S.
Right arrow Articles by KERR, L. D.

Am. J. Respir. Crit. Care Med., Volume 162, Number 3, September 2000, 1095-1101

Multiorgan Nuclear Factor Kappa B Activation in a Transgenic Mouse Model of Systemic Inflammation

TIMOTHY S. BLACKWELL, FIONA E. YULL, CHIH-LI CHEN, ANNAPURNA VENKATAKRISHNAN, THOMAS R. BLACKWELL, DONNA J. HICKS, LISA H. LANCASTER, JOHN W. CHRISTMAN, and LAWRENCE D. KERR

Department of Medicine, Division of Allergy, Pulmonary, and Critical Care Medicine, Departments of Microbiology and Immunology, and Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee; and Department of Veterans Affairs Medical Center, Nashville, Tennessee

We utilized a line of transgenic mice expressing Photinus luciferase complementary DNA (cDNA) under the control of a nuclear factor kappa B (NF-kappa B)-dependent promoter (from the 5' human immunodeficiency virus-1 [HIV-1] long terminal repeat) to examine the role of NF-kappa B activation in the pathogenesis of systemic inflammation induced by bacterial endotoxin (lipopolysaccharide [LPS]). After intraperitoneal injection of E. coli LPS, these mice displayed a time- and dose-dependent, organ-specific pattern of luciferase expression, showing that NF-kappa B-dependent gene transcription is transiently activated in multiple organs by systemic LPS administration. Luciferase expression in liver could be specifically blocked by intravenous administration of replication-deficient adenoviral vectors expressing a dominant inhibitor of NF-kappa B (Ikappa B-alpha DN), confirming that luciferase gene expression is a surrogate marker for NF-kappa B activation in this line of mice. After treatment with intraperitoneal LPS, the mice were found to have increased lung tissue messenger RNA (mRNA) expression of a variety of cytokines that are thought to be NF-kappa B-dependent, as well as elevated serum concentrations of presumed NF-kappa B-dependent cytokines. In lung tissue homogenates, a close correlation was identified between luciferase activity and KC levels. These studies show that systemic treatment with LPS orchestrates a multiorgan NF-kappa B-dependent response that likely regulates the pathobiology of systemic inflammation.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Han, M. Joo, M. B. Everhart, J. W. Christman, F. E. Yull, and T. S. Blackwell
Myeloid cells control termination of lung inflammation through the NF-{kappa}B pathway
Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L320 - L327.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. S. Musiek, J. D. Brooks, M. Joo, E. Brunoldi, A. Porta, G. Zanoni, G. Vidari, T. S. Blackwell, T. J. Montine, G. L. Milne, et al.
Electrophilic Cyclopentenone Neuroprostanes Are Anti-inflammatory Mediators Formed from the Peroxidation of the {omega}-3 Polyunsaturated Fatty Acid Docosahexaenoic Acid
J. Biol. Chem., July 18, 2008; 283(29): 19927 - 19935.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Yoon, Z. Korade, and B. D. Carter
Protein Kinase A-Induced Phosphorylation of the p65 Subunit of Nuclear Factor-{kappa}B Promotes Schwann Cell Differentiation into a Myelinating Phenotype
J. Neurosci., April 2, 2008; 28(14): 3738 - 3746.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
G. T. Stathopoulos, T. P. Sherrill, W. Han, R. T. Sadikot, F. E. Yull, T. S. Blackwell, and B. Fingleton
Host Nuclear Factor-{kappa}B Activation Potentiates Lung Cancer Metastasis
Mol. Cancer Res., March 1, 2008; 6(3): 364 - 371.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. Cepinskas, K. Katada, A. Bihari, and R. F. Potter
Carbon monoxide liberated from carbon monoxide-releasing molecule CORM-2 attenuates inflammation in the liver of septic mice
Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G184 - G191.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. T. Stathopoulos, T. P. Sherrill, D.-S. Cheng, R. M. Scoggins, W. Han, V. V. Polosukhin, L. Connelly, F. E. Yull, B. Fingleton, and T. S. Blackwell
Epithelial NF-{kappa}B activation promotes urethane-induced lung carcinogenesis
PNAS, November 20, 2007; 104(47): 18514 - 18519.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. C. Glasgow, S. Ramachandran, T. S. Blackwell, T. Mohanakumar, and W. C. Chapman
Interleukin-1beta is the primary initiator of pulmonary inflammation following liver injury in mice
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L491 - L496.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D.-s. Cheng, W. Han, S. M. Chen, T. P. Sherrill, M. Chont, G.-Y. Park, J. R. Sheller, V. V. Polosukhin, J. W. Christman, F. E. Yull, et al.
Airway Epithelium Controls Lung Inflammation and Injury through the NF-{kappa}B Pathway
J. Immunol., May 15, 2007; 178(10): 6504 - 6513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. Mathew, G. Y. Park, H. Cao, A. C. Azim, X. Wang, R. B. Van Breemen, R. T. Sadikot, and J. W. Christman
Inhibitory {kappa}B Kinase 2 Activates Airway Epithelial Cells to Stimulate Bone Marrow Macrophages
Am. J. Respir. Cell Mol. Biol., May 1, 2007; 36(5): 562 - 572.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. M. Alvira, A. Abate, G. Yang, P. A. Dennery, and M. Rabinovitch
Nuclear Factor-{kappa}B Activation in Neonatal Mouse Lung Protects against Lipopolysaccharide-induced Inflammation
Am. J. Respir. Crit. Care Med., April 15, 2007; 175(8): 805 - 815.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
W. Zhou, T. S. Blackwell, K. Goleniewska, J. F. O'Neal, G. A. FitzGerald, M. Lucitt, R. M. Breyer, and R. S. Peebles Jr.
Prostaglandin I2 analogs inhibit Th1 and Th2 effector cytokine production by CD4 T cells
J. Leukoc. Biol., March 1, 2007; 81(3): 809 - 817.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. Zhou, K. Hashimoto, K. Goleniewska, J. F. O'Neal, S. Ji, T. S. Blackwell, G. A. FitzGerald, K. M. Egan, M. W. Geraci, and R. S. Peebles Jr.
Prostaglandin I2 Analogs Inhibit Proinflammatory Cytokine Production and T Cell Stimulatory Function of Dendritic Cells
J. Immunol., January 15, 2007; 178(2): 702 - 710.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. T. Sadikot, H. Zeng, M. Joo, M. B. Everhart, T. P. Sherrill, B. Li, D.-s. Cheng, F. E. Yull, J. W. Christman, and T. S. Blackwell
Targeted Immunomodulation of the NF-{kappa}B Pathway in Airway Epithelium Impacts Host Defense against Pseudomonas aeruginosa.
J. Immunol., April 15, 2006; 176(8): 4923 - 4930.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. B. Everhart, W. Han, T. P. Sherrill, M. Arutiunov, V. V. Polosukhin, J. R. Burke, R. T. Sadikot, J. W. Christman, F. E. Yull, and T. S. Blackwell
Duration and Intensity of NF-{kappa}B Activity Determine the Severity of Endotoxin-Induced Acute Lung Injury.
J. Immunol., April 15, 2006; 176(8): 4995 - 5005.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
R. T. Sadikot and T. S. Blackwell
Bioluminescence Imaging
Proceedings of the ATS, December 1, 2005; 2(6): 537 - 540.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. L. Mora, J. LaVoy, M. McKean, A. Stecenko, K. L. Brigham, R. Parker, and M. Rojas
Prevention of NF-{kappa}B activation in vivo by a cell-permeable NF-{kappa}B inhibitor peptide
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L536 - L544.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. B. Everhart, W. Han, K. S. Parman, V. V. Polosukhin, H. Zeng, R. T. Sadikot, B. Li, F. E. Yull, J. W. Christman, and T. S. Blackwell
Intratracheal administration of liposomal clodronate accelerates alveolar macrophage reconstitution following fetal liver transplantation
J. Leukoc. Biol., February 1, 2005; 77(2): 173 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Rojas, C. R. Woods, A. L. Mora, J. Xu, and K. L. Brigham
Endotoxin-induced lung injury in mice: structural, functional, and biochemical responses
Am J Physiol Lung Cell Mol Physiol, February 1, 2005; 288(2): L333 - L341.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. A. Altemeier, G. Matute-Bello, C. W. Frevert, Y. Kawata, O. Kajikawa, T. R. Martin, and R. W. Glenny
Mechanical ventilation with moderate tidal volumes synergistically increases lung cytokine response to systemic endotoxin
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L533 - L542.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
W. S. Madorin, T. Rui, N. Sugimoto, O. Handa, G. Cepinskas, and P. R. Kvietys
Cardiac Myocytes Activated by Septic Plasma Promote Neutrophil Transendothelial Migration: Role of Platelet-Activating Factor and the Chemokines LIX and KC
Circ. Res., April 16, 2004; 94(7): 944 - 951.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. T. Sadikot, H. Zeng, F. E. Yull, B. Li, D.-s. Cheng, D. S. Kernodle, E. D. Jansen, C. H. Contag, B. H. Segal, S. M. Holland, et al.
p47phox Deficiency Impairs NF-{kappa}B Activation and Host Defense in Pseudomonas Pneumonia
J. Immunol., February 1, 2004; 172(3): 1801 - 1808.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. E. Poynter, C. G. Irvin, and Y. M. W. Janssen-Heininger
A Prominent Role for Airway Epithelial NF-{kappa}B Activation in Lipopolysaccharide-Induced Airway Inflammation
J. Immunol., June 15, 2003; 170(12): 6257 - 6265.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
F. E. Yull, W. Han, E. D. Jansen, M. B. Everhart, R. T. Sadikot, J. W. Christman, and T. S. Blackwell
Bioluminescent Detection of Endotoxin Effects on HIV-1 LTR-driven Transcription in Vivo
J. Histochem. Cytochem., June 1, 2003; 51(6): 741 - 749.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
J. Macdonald, H. F. Galley, and N. R. Webster
Oxidative stress and gene expression in sepsis
Br. J. Anaesth., February 1, 2003; 90(2): 221 - 232.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. T. Sadikot, W. Han, M. B. Everhart, O. Zoia, R. S. Peebles, E. D. Jansen, F. E. Yull, J. W. Christman, and T. S. Blackwell
Selective I{kappa}B Kinase Expression in Airway Epithelium Generates Neutrophilic Lung Inflammation
J. Immunol., January 15, 2003; 170(2): 1091 - 1098.
[Abstract] [Full Text] [PDF]

Home page
 Annals of Clinical & Laboratory ScienceHome page
Z. Liu, Y. Yu, Y. Jiang, and J. Li
Growth Hormone Increases Lung NF-{kappa}B Activation and Lung Microvascular Injury Induced by Lipopolysaccharide in Rats
Ann. Clin. Lab. Sci., April 1, 2002; 32(2): 164 - 170.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Carlsen, J. O. Moskaug, S. H. Fromm, and R. Blomhoff
In Vivo Imaging of NF-{kappa}B Activity
J. Immunol., February 1, 2002; 168(3): 1441 - 1446.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. Tobin
Taxonomy of AJRCCM, a New Series, and a Medley of Metaphors
Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): 1333 - 1335.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Critical Care Medicine in AJRCCM 2000
Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): 1347 - 1361.
[Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. A. Koay, J. W. Christman, B. H. Segal, A. Venkatakrishnan, T. R. Blackwell, S. M. Holland, and T. S. Blackwell
Impaired Pulmonary NF-{kappa}B Activation in Response to Lipopolysaccharide in NADPH Oxidase-Deficient Mice
Infect. Immun., October 1, 2001; 69(10): 5991 - 5996.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. T. SADIKOT, E. D. JANSEN, T. R. BLACKWELL, O. ZOIA, F. YULL, J. W. CHRISTMAN, and T. S. BLACKWELL
High-Dose Dexamethasone Accentuates Nuclear Factor-kappa B Activation in Endotoxin-Treated Mice
Am. J. Respir. Crit. Care Med., September 1, 2001; 164(5): 873 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. M. Brantley, C.-L. Chen, R. S. Muraoka, P. B. Bushdid, J. L. Bradberry, F. Kittrell, D. Medina, L. M. Matrisian, L. D. Kerr, and F. E. Yull
Nuclear Factor-{kappa}B (NF-{kappa}B) Regulates Proliferation and Branching in Mouse Mammary Epithelium
Mol. Biol. Cell, May 1, 2001; 12(5): 1445 - 1455.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2000 American Thoracic Society