Am. J. Respir. Crit. Care Med., Volume 162, Number 3, September 2000, 1052-1057

Lung Volume Reduction Surgery Does Not Improve Diaphragmatic Contractile Properties or Atrophy in Hamsters with Elastase-induced Emphysema

ERIC MARCHAND, PAUL DE LEYN, GHISLAINE GAYAN-RAMIREZ, FRANTISEK PALECEK, VERA de BOCK, RENÉ DOM, and MARC DECRAMER

Respiratory Muscle Research Unit, Laboratory of Pneumology and Respiratory Division, Thoracic Surgery Division, and Neuropathology Department, University Hospitals, Katholieke Universiteit Leuven, Leuven, Belgium; and Respiratory Division, Mont-Godinne University Hospital, Université Catholique de Louvain, Yvoir, Belgium

It is claimed that lung volume reduction surgery (LVRS) improves inspiratory muscle function. As diaphragm structure and function are not directly appraisable in patients, we studied the effects of LVRS on the diaphragm in vitro contractile properties and morphology in hamsters with elastase-induced emphysema. Four months after intratracheal instillation of elastase (40 U/100 g), hamsters underwent either bilateral LVRS (LVRS, n = 11) or a sham operation (SHAM, n = 8). Four animals died during the perioperative period in LVRS (n = 7). Hamsters instilled with saline served as control (CTL, n = 8). Animals were studied at the age of 9 mo. LVRS was associated with a significant 25% decrease in functional residual capacity compared to SHAM (p < 0.05). Compared with CTL, LVRS and SHAM showed a significant 18% and 14% reduction in diaphragm mass, respectively (p = 0.02). LVRS had a significantly decreased twitch tension compared to CTL and SHAM (p < 0.01). Both LVRS and SHAM showed increased resistance to muscle fatigue compared with CTL. The histochemical analysis revealed a significant shift from type IIx/b toward type IIa fibers in LVRS and SHAM compared with CTL. In conclusion, emphysema is associated with functional adaptations but LVRS does not appear to beneficially alter the diaphragm contractile and morphological characteristics in hamsters with elastase-induced emphysema.

This article has been cited by other articles:

				M. Estenne Effect of lung transplant and volume reduction surgery on respiratory muscle function J Appl Physiol, September 1, 2009; 107(3): 977 - 986. [Abstract] [Full Text] [PDF]

				T. L. Clanton and S. Levine Respiratory muscle fiber remodeling in chronic hyperinflation: dysfunction or adaptation? J Appl Physiol, July 1, 2009; 107(1): 324 - 335. [Abstract] [Full Text] [PDF]

				H. E. Fessler, S. M. Scharf, E. P. Ingenito, R. J. McKenna Jr., and A. Sharafkhaneh Physiologic Basis for Improved Pulmonary Function after Lung Volume Reduction Proceedings of the ATS, May 1, 2008; 5(4): 416 - 420. [Abstract] [Full Text] [PDF]

				G. Borzone, L. Liberona, P. Olmos, C. Saez, M. Meneses, T. Reyes, R. Moreno, and C. Lisboa Rat and hamster species differences in susceptibility to elastase-induced pulmonary emphysema relate to differences in elastase inhibitory capacity Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1342 - R1349. [Abstract] [Full Text] [PDF]

				N. Shigemura, Y. Sawa, S. Mizuno, M. Ono, M. Minami, M. Okumura, T. Nakamura, Y. Kaneda, and H. Matsuda Induction of Compensatory Lung Growth in Pulmonary Emphysema Improves Surgical Outcomes in Rats Am. J. Respir. Crit. Care Med., June 1, 2005; 171(11): 1237 - 1245. [Abstract] [Full Text] [PDF]

				M. I. Lewis, M. Fournier, X. Da, H. Li, Z. Mosenifar, R. J. McKenna Jr, and A. H. Cohen Short-term Influences of Lung Volume Reduction Surgery on the Diaphragm in Emphysematous Hamsters Am. J. Respir. Crit. Care Med., October 1, 2004; 170(7): 753 - 759. [Abstract] [Full Text] [PDF]

				M. Decramer Treatment of chronic respiratory failure: lung volume reduction surgery versus rehabilitation Eur. Respir. J., November 16, 2003; 22(47_suppl): 47s - 56s. [Abstract] [Full Text] [PDF]

				F. Laghi and M. J. Tobin Disorders of the Respiratory Muscles Am. J. Respir. Crit. Care Med., July 1, 2003; 168(1): 10 - 48. [Abstract] [Full Text] [PDF]

				M. J. Tobin Compliance (COMmunicate PLease wIth Less Abbreviations, Noun Clusters, and Exclusiveness) Am. J. Respir. Crit. Care Med., December 15, 2002; 166(12): 1534 - 1536. [Full Text] [PDF]

				V. Reynders, E. Marchand, G. Gayan-Ramirez, P. De Leyn, G. Verhoeven, and M. Decramer Alterations in gene expression in hamster diaphragm after emphysema and lung volume reduction surgery Eur. Respir. J., June 1, 2002; 19(6): 1064 - 1071. [Abstract] [Full Text] [PDF]

				E. Marchand, P. De Leyn, G. Gayan-Ramirez, F. Palecek, E. Verbeken, and M. Decramer Effects of lung volume reduction surgery in hamsters with elastase-induced emphysema Eur. Respir. J., March 1, 2002; 19(3): 422 - 428. [Abstract] [Full Text] [PDF]

				J. B. Shrager, D.-K. Kim, Y. J. Hashmi, H. H. Stedman, J. Zhu, L. R. Kaiser, and S. Levine Sarcomeres Are Added in Series to Emphysematous Rat Diaphragm After Lung Volume Reduction Surgery Chest, January 1, 2002; 121(1): 210 - 215. [Abstract] [Full Text] [PDF]

				M. J. TOBIN Chronic Obstructive Pulmonary Disease, Pollution, Pulmonary Vascular Disease, Transplantation, Pleural Disease, and Lung Cancer in AJRCCM 2000 Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): 1789 - 1804. [Full Text] [PDF]