help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by YAMADA, T.
Right arrow Articles by NAKANO, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by YAMADA, T.
Right arrow Articles by NAKANO, H.

Am. J. Respir. Crit. Care Med., Volume 162, Number 2, August 2000, 707-715

Enhanced Expression of Hepatocyte Growth Factor by Pulmonary Ischemia-Reperfusion Injury in the Rat

TAKATSUGU YAMADA, MICHIYOSHI HISANAGA, YOSHIYUKI NAKAJIMA, SHINYA MIZUNO, KUNIO MATSUMOTO, TOSHIKAZU NAKAMURA, and HIROSHIGE NAKANO

First Department of Surgery, Nara Medical University, Nara; and Division of Biochemistry, Department of Oncology, Biomedical Research Center, Osaka University Medical School, Osaka, Japan

Hepatocyte growth factor (HGF) has recently been noted to function as a pulmotrophic factor for lung regeneration. The present study was conducted to determine if HGF would be induced in a rat model of pulmonary ischemia-reperfusion (IR) injury, which was established by occlusion of the left lung, and to examine the significance of HGF in subsequent lung repair. The sham-operated rats underwent simple thoracotomy in which the lung was not clamped. We measured the plasma and the tissue levels of HGF by enzyme-linked immunosorbent assays, and the expression of HGF mRNA by Northern blotting. The plasma HGF level was markedly elevated after pulmonary ischemia and reached the peak value on the third postoperative day, being 5-fold higher than that of the sham-operated rats. HGF mRNA expression and the tissue HGF levels were augmented twofold in the ischemic reperfused lung. Immunohistochemical analysis revealed that the infiltrating alveolar macrophages were intensely stained for HGF. DNA synthesis of alveolar epithelial cells, as identified by proliferating cell nuclear antigen (PCNA) staining, was 3-fold higher in the reperfused lung than in the sham-operated lung. Notably, HGF-neutralizing treatment with an anti-HGF antibody reduced DNA synthesis of alveolar epithelial cells in the reperfused lung and aggravated lung injury. This study shows that HGF was induced in the ischemic reperfused lung and may play an important role in regeneration of an injured lung after pulmonary IR.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Quesnel, S. Marchand-Adam, A. Fabre, J. Marchal-Somme, I. Philip, S. Lasocki, V. Lecon, B. Crestani, and M. Dehoux
Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury
Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L334 - L343.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. Skibinski, J. S. Elborn, and M. Ennis
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L69 - L76.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Marchand-Adam, A. Fabre, A. A. Mailleux, J. Marchal, C. Quesnel, H. Kataoka, M. Aubier, M. Dehoux, P. Soler, and B. Crestani
Defect of Pro-Hepatocyte Growth Factor Activation by Fibroblasts in Idiopathic Pulmonary Fibrosis
Am. J. Respir. Crit. Care Med., July 1, 2006; 174(1): 58 - 66.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
E. Dikmen, M. Kara, U. Kisa, C. Atinkaya, S. Han, and U. Sakinci
Human hepatocyte growth factor levels in patients undergoing thoracic operations
Eur. Respir. J., January 1, 2006; 27(1): 73 - 76.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. McKinnon, E. Gherardi, M. Reidy, and D. Bowyer
Hepatocyte Growth Factor/Scatter Factor and MET Are Involved in Arterial Repair and Atherogenesis
Am. J. Pathol., January 1, 2006; 168(1): 340 - 348.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Padela, J. Cabacungan, S. Shek, R. Belcastro, M. Yi, R. P. Jankov, and A. K. Tanswell
Hepatocyte Growth Factor Is Required for Alveologenesis in the Neonatal Rat
Am. J. Respir. Crit. Care Med., October 1, 2005; 172(7): 907 - 914.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Shigemura, Y. Sawa, S. Mizuno, M. Ono, M. Minami, M. Okumura, T. Nakamura, Y. Kaneda, and H. Matsuda
Induction of Compensatory Lung Growth in Pulmonary Emphysema Improves Surgical Outcomes in Rats
Am. J. Respir. Crit. Care Med., June 1, 2005; 171(11): 1237 - 1245.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
N. Shigemura, Y. Sawa, S. Mizuno, M. Ono, M. Ohta, T. Nakamura, Y. Kaneda, and H. Matsuda
Amelioration of Pulmonary Emphysema by In Vivo Gene Transfection With Hepatocyte Growth Factor in Rats
Circulation, March 22, 2005; 111(11): 1407 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
M. Ono, Y. Sawa, N. Fukushima, H. Suhara, T. Nakamura, C. Yokoyama, T. Tanabe, and H. Matsuda
Gene transfer of hepatocyte growth factor with prostacyclin synthase in severe pulmonary hypertension of rats
Eur. J. Cardiothorac. Surg., December 1, 2004; 26(6): 1092 - 1097.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
M. Ono, Y. Sawa, S. Mizuno, N. Fukushima, H. Ichikawa, K. Bessho, T. Nakamura, and H. Matsuda
Hepatocyte Growth Factor Suppresses Vascular Medial Hyperplasia and Matrix Accumulation in Advanced Pulmonary Hypertension of Rats
Circulation, November 2, 2004; 110(18): 2896 - 2902.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. M. Day, G. Thiel, J. Lum, R. D. Chevere, Y. Yang, J. Stevens, L. Sibert, and B. L. Fanburg
Hepatocyte Growth Factor Regulates Angiotensin Converting Enzyme Expression
J. Biol. Chem., March 5, 2004; 279(10): 8792 - 8801.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
N. Hattori, S. Mizuno, Y. Yoshida, K. Chin, M. Mishima, T. H. Sisson, R. H. Simon, T. Nakamura, and M. Miyake
The Plasminogen Activation System Reduces Fibrosis in the Lung by a Hepatocyte Growth Factor-Dependent Mechanism
Am. J. Pathol., March 1, 2004; 164(3): 1091 - 1098.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Wang, Y. Zhou, H. P. Kim, R. Song, R. Zarnegar, S. W. Ryter, and A. M. K. Choi
Hepatocyte Growth Factor Protects against Hypoxia/Reoxygenation-induced Apoptosis in Endothelial Cells
J. Biol. Chem., February 13, 2004; 279(7): 5237 - 5243.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Amano, K. Morimoto, M. Senba, H. Wang, Y. Ishida, A. Kumatori, H. Yoshimine, K. Oishi, N. Mukaida, and T. Nagatake
Essential Contribution of Monocyte Chemoattractant Protein-1/C-C Chemokine Ligand-2 to Resolution and Repair Processes in Acute Bacterial Pneumonia
J. Immunol., January 1, 2004; 172(1): 398 - 409.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Sakamaki, K. Matsumoto, S. Mizuno, S. Miyoshi, H. Matsuda, and T. Nakamura
Hepatocyte Growth Factor Stimulates Proliferation of Respiratory Epithelial Cells during Postpneumonectomy Compensatory Lung Growth in Mice
Am. J. Respir. Cell Mol. Biol., May 1, 2002; 26(5): 525 - 533.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Critical Care Medicine in AJRCCM 2000
Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): 1347 - 1361.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2000 American Thoracic Society 		  ATS Clinical Skills Tests