help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by KITAMURA, T.
Right arrow Articles by NAKATA, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by KITAMURA, T.
Right arrow Articles by NAKATA, K.

Am. J. Respir. Crit. Care Med., Volume 162, Number 2, August 2000, 658-662

Serological Diagnosis of Idiopathic Pulmonary Alveolar Proteinosis

TAKAYUKI KITAMURA, KANJI UCHIDA, NAOHIKO TANAKA, TOMOKO TSUCHIYA, JUNICHI WATANABE, YOSHITSUGU YAMADA, KAZUO HANAOKA, JOHN F. SEYMOUR, OTTO D. SCHOCH, IAN DOYLE, YOSHIKAZU INOUE, MITSUNORI SAKATANI, SHOJI KUDOH, ARATA AZUMA, TOSHIHIRO NUKIWA, TOMOYUKI TOMITA, MAKOTO KATAGIRI, AKIRA FUJITA, ATSUYUKI KURASHIMA, SHIRO KANEGASAKI, and KOH NAKATA

Department of Pulmonary Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan; School of Medicine, Kitasato University, Kanagawa, Japan; Department of Anesthesiology, Faculty of Medicine, University of Tokyo, Tokyo, Japan; Ludwig Institute for Cancer Research, Parkville, Australia; University Hospital, Zurich, Switzerland; Flinders University, Bedford Park, Australia; National Kinki-Chuo Hospital, Osaka, Japan; Nippon Medical School, Tokyo, Japan; Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan; Tokyo Metropolitan Fuchu Hospital, Tokyo, Japan; and National Tokyo Hospital, Tokyo, Japan

Previously, we reported the specific occurrence of neutralizing autoantibodies against granulocyte-macrophage colony-stimulating factor (GM-CSF) in the bronchoalveolar lavage fluid from 11 Japanese patients with idiopathic pulmonary alveolar proteinosis (I-PAP). The autoantibody was also detected in sera from all 5 I-PAP patients examined. To determine that the existence of the autoantibody is not limited to the Japanese patients, we examined sera from 24 I-PAP patients in five countries and showed that the autoantibody was consistently and specifically present in such patients. Thus, detection of the autoantibody in sera can be used for diagnosis of I-PAP. To establish a simple and convenient method for diagnosis of I-PAP, we developed a novel latex agglutination test using latex beads coupled with recombinant human GM-CSF. GM-CSF binding proteins isolated from the sera using the latex beads were identified as the autoantibodies of IgG1 and IgG2. The titer of the autoantibody determined by this test correlated with that determined by ELISA. Agglutination was positive in 300-fold diluted sera from all 24 I-PAP patients, but negative in sera from four secondary PAP patients, two congenital PAP patients, 40 patients with other lung diseases, and 38 of 40 normal subjects. These results establish that the latex agglutination test is a reliable method for serological diagnosis of I-PAP with high sensitivity (100%) and specificity (98%).




This article has been cited by other articles:


Home page
 ChestHome page
H. Ishii, B. C. Trapnell, R. Tazawa, Y. Inoue, M. Akira, Y. Kogure, K. Tomii, T. Takada, M. Hojo, T. Ichiwata, et al.
Comparative Study of High-Resolution CT Findings Between Autoimmune and Secondary Pulmonary Alveolar Proteinosis
Chest, November 1, 2009; 136(5): 1348 - 1355.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
R. Borie, M-P. Debray, C. Laine, M. Aubier, and B. Crestani
Rituximab therapy in autoimmune pulmonary alveolar proteinosis
Eur. Respir. J., June 1, 2009; 33(6): 1503 - 1506.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Uchida, K. Nakata, T. Suzuki, M. Luisetti, M. Watanabe, D. E. Koch, C. A. Stevens, D. C. Beck, L. A. Denson, B. C. Carey, et al.
Granulocyte/macrophage-colony-stimulating factor autoantibodies and myeloid cell immune functions in healthy subjects
Blood, March 12, 2009; 113(11): 2547 - 2556.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
T. Suzuki, T. Sakagami, B. K. Rubin, L. M. Nogee, R. E. Wood, S. L. Zimmerman, T. Smolarek, M. K. Dishop, S. E. Wert, J. A. Whitsett, et al.
Familial pulmonary alveolar proteinosis caused by mutations in CSF2RA
J. Exp. Med., November 24, 2008; 205(12): 2703 - 2710.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Y. Inoue, B. C. Trapnell, R. Tazawa, T. Arai, T. Takada, N. Hizawa, Y. Kasahara, K. Tatsumi, M. Hojo, T. Ichiwata, et al.
Characteristics of a Large Cohort of Patients with Autoimmune Pulmonary Alveolar Proteinosis in Japan
Am. J. Respir. Crit. Care Med., April 1, 2008; 177(7): 752 - 762.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
F-C Lin, G-D Chang, M-S Chern, Y-C Chen, and S-C Chang
Clinical significance of anti-GM-CSF antibodies in idiopathic pulmonary alveolar proteinosis
Thorax, June 1, 2006; 61(6): 528 - 534.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
M. E. Wylam, R. Ten, U. B. S. Prakash, H. F. Nadrous, M. L. Clawson, and P. M. Anderson
Aerosol granulocyte-macrophage colony-stimulating factor for pulmonary alveolar proteinosis.
Eur. Respir. J., March 1, 2006; 27(3): 585 - 593.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
T. L. Bonfield, N. John, B. P. Barna, M. S. Kavuru, M. J. Thomassen, and B. Yen-Lieberman
Multiplexed Particle-Based Anti-Granulocyte Macrophage Colony Stimulating Factor Assay Used as Pulmonary Diagnostic Test
Clin. Vaccine Immunol., July 1, 2005; 12(7): 821 - 824.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. Tazawa, E. Hamano, T. Arai, H. Ohta, O. Ishimoto, K. Uchida, M. Watanabe, J. Saito, M. Takeshita, Y. Hirabayashi, et al.
Granulocyte-Macrophage Colony-Stimulating Factor and Lung Immunity in Pulmonary Alveolar Proteinosis
Am. J. Respir. Crit. Care Med., May 15, 2005; 171(10): 1142 - 1149.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
P Latzin, M Tredano, Y Wust, J de Blic, T Nicolai, B Bewig, F Stanzel, D Kohler, M Bahuau, and M Griese
Anti-GM-CSF antibodies in paediatric pulmonary alveolar proteinosis
Thorax, January 1, 2005; 60(1): 39 - 44.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
F. Brasch, M. Griese, M. Tredano, G. Johnen, M. Ochs, C. Rieger, S. Mulugeta, K.M. Muller, M. Bahuau, and M.F. Beers
Interstitial lung disease in a baby with a de novo mutation in the SFTPC gene
Eur. Respir. J., July 1, 2004; 24(1): 30 - 39.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
A. Perez IV and R. M. Rogers
Enhanced Alveolar Clearance With Chest Percussion Therapy and Positional Changes During Whole-Lung Lavage for Alveolar Proteinosis
Chest, June 1, 2004; 125(6): 2351 - 2356.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Uchida, K. Nakata, B. C. Trapnell, T. Terakawa, E. Hamano, A. Mikami, I. Matsushita, J. F. Seymour, M. Oh-eda, I. Ishige, et al.
High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis
Blood, February 1, 2004; 103(3): 1089 - 1098.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
B. C. Trapnell, J. A. Whitsett, and K. Nakata
Pulmonary Alveolar Proteinosis
N. Engl. J. Med., December 25, 2003; 349(26): 2527 - 2539.
[Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. L. Bonfield, C. F. Farver, B. P. Barna, A. Malur, S. Abraham, B. Raychaudhuri, M. S. Kavuru, and M. J. Thomassen
Peroxisome Proliferator-Activated Receptor-{gamma} Is Deficient in Alveolar Macrophages from Patients with Alveolar Proteinosis
Am. J. Respir. Cell Mol. Biol., December 1, 2003; 29(6): 677 - 682.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. S. Kavuru, T. L. Bonfield, M. J. Thomassen, J. F. Seymour, and J. J. Presneill
Plasmapheresis, GM-CSF, and alveolar proteinosis
Am. J. Respir. Crit. Care Med., April 1, 2003; 167(7): 1036 - 1037.
[Full Text]

Home page
 ThoraxHome page
J F Seymour, I R Doyle, K Nakata, J J Presneill, O D Schoch, E Hamano, K Uchida, R Fisher, and A R Dunn
Relationship of anti-GM-CSF antibody concentration, surfactant protein A and B levels, and serum LDH to pulmonary parameters and response to GM-CSF therapy in patients with idiopathic alveolar proteinosis
Thorax, March 1, 2003; 58(3): 252 - 257.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P.-Y. Berclaz, Z. Zsengeller, Y. Shibata, K. Otake, S. Strasbaugh, J. A. Whitsett, and B. C. Trapnell
Endocytic Internalization of Adenovirus, Nonspecific Phagocytosis, and Cytoskeletal Organization Are Coordinately Regulated in Alveolar Macrophages by GM-CSF and PU.1
J. Immunol., December 1, 2002; 169(11): 6332 - 6342.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. L. Bonfield, D. Russell, S. Burgess, A. Malur, M. S. Kavuru, and M. J. Thomassen
Autoantibodies against Granulocyte Macrophage Colony-Stimulating Factor Are Diagnostic for Pulmonary Alveolar Proteinosis
Am. J. Respir. Cell Mol. Biol., October 1, 2002; 27(4): 481 - 486.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. S. Kavuru and M. Popovich
Therapeutic Whole Lung Lavage : A Stop-Gap Therapy for Alveolar Proteinosis
Chest, October 1, 2002; 122(4): 1123 - 1124.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. F. Seymour and J. J. Presneill
Pulmonary Alveolar Proteinosis: Progress in the First 44 Years
Am. J. Respir. Crit. Care Med., July 15, 2002; 166(2): 215 - 235.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
O D Schoch, U Schanz, M Koller, K Nakata, J F Seymour, E W Russi, and A Boehler
BAL findings in a patient with pulmonary alveolar proteinosis successfully treated with GM-CSF
Thorax, March 1, 2002; 57(3): 277 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Tuberculosis, Lung Infections, and Interstitial Lung Disease in AJRCCM 2000
Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): 1774 - 1788.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2000 American Thoracic Society 		  Portfolio of grants