help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by GAUVREAU, G. M.
Right arrow Articles by O'BYRNE, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by GAUVREAU, G. M.
Right arrow Articles by O'BYRNE, P. M.

Am. J. Respir. Crit. Care Med., Volume 161, Number 5, May 2000, 1473-1478

Increased Numbers of Both Airway Basophils and Mast Cells in Sputum after Allergen Inhalation Challenge of Atopic Asthmatics

GAIL M. GAUVREAU, JONG M. LEE, RICHARD M. WATSON, ANNE-MARIE A. IRANI, LAWRENCE B. SCHWARTZ, and PAUL M. O'BYRNE

Asthma Research Group, St. Joseph's Hospital, and Department of Medicine, McMaster University, Hamilton, Ontario, Canada; and Departments of Pediatrics and Medicine, Virginia Commonwealth University, Richmond, Virginia

Mast cells and basophils are metachromatic cells that participate in allergic inflammation. Allergen challenge to the airways of atopic asthmatic individuals increases levels of metachromatic cells, which may reflect an increase in mast cells, basophils, or both. We conducted a study to characterize the kinetics of basophil and mast cell recruitment to the airways of atopic asthmatic subjects after allergen inhalation challenge, using monoclonal antibodies specific for each type of cell. Of 19 subjects, 14 developed both early- and late-phase asthmatic responses (dual responders [DRs]), whereas five developed only early asthmatic responses (early responders [ERs]) after allergen inhalation. There was a significant increase in the number of sputum eosinophils (p < 0.002) and basophils (p < 0.002) at 7 h and 24 h after challenge in both ERs and DRs. There was also a significant increase in the number of activated eosinophils (p = 0.00002) and mast cells (p = 0.009) in sputum at 7 h and 24 h after challenge in DRs, but not in ERs (p > 0.4). DRs had a significantly higher number of allergen-induced sputum basophils than did ERs (p < 0.01), and sputum basophils correlated significantly with airway hyperresponsiveness (AHR) to methacholine at 24 h after challenge (r = 0.66, p = 0.002). DRs tended to have higher allergen-induced basophil levels than did ERs, which may contribute to the observed AHR.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
G. M. Gauvreau, L. P. Boulet, D. W. Cockcroft, A. Baatjes, J. Cote, F. Deschesnes, B. Davis, T. Strinich, K. Howie, M. Duong, et al.
Antisense Therapy against CCR3 and the Common Beta Chain Attenuates Allergen-induced Eosinophilic Responses
Am. J. Respir. Crit. Care Med., May 1, 2008; 177(9): 952 - 958.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Obata, K. Mukai, Y. Tsujimura, K. Ishiwata, Y. Kawano, Y. Minegishi, N. Watanabe, and H. Karasuyama
Basophils are essential initiators of a novel type of chronic allergic inflammation
Blood, August 1, 2007; 110(3): 913 - 920.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. J. Lee and M. P. McGarry
When is a mouse basophil not a basophil?
Blood, February 1, 2007; 109(3): 859 - 861.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
J. A. Denburg and S. F. van Eeden
Bone marrow progenitors in inflammation and repair: new vistas in respiratory biology and pathophysiology.
Eur. Respir. J., March 1, 2006; 27(3): 441 - 445.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Iikura, M. Ebisawa, M. Yamaguchi, H. Tachimoto, K. Ohta, K. Yamamoto, and K. Hirai
Transendothelial Migration of Human Basophils
J. Immunol., October 15, 2004; 173(8): 5189 - 5195.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. L. Thurmond, P. J. Desai, P. J. Dunford, W.-P. Fung-Leung, C. L. Hofstra, W. Jiang, S. Nguyen, J. P. Riley, S. Sun, K. N. Williams, et al.
A Potent and Selective Histamine H4 Receptor Antagonist with Anti-Inflammatory Properties
J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 404 - 413.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. C. Dorman, A. Efthimiadis, I. Babirad, R. M. Watson, J. A. Denburg, F. E. Hargreave, P. M. O'Byrne, and R. Sehmi
Sputum CD34+IL-5R{alpha}+ Cells Increase after Allergen: Evidence for In Situ Eosinophilopoiesis
Am. J. Respir. Crit. Care Med., March 1, 2004; 169(5): 573 - 577.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. C. Dorman, R. Sehmi, G. M. Gauvreau, R. M. Watson, R. Foley, G. L. Jones, J. A. Denburg, M. D. Inman, and P. M. O'Byrne
Kinetics of Bone Marrow Eosinophilopoiesis and Associated Cytokines after Allergen Inhalation
Am. J. Respir. Crit. Care Med., March 1, 2004; 169(5): 565 - 572.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. K. Ikeda, M. Miller, J. Nayar, L. Walker, J. Y. Cho, K. McElwain, S. McElwain, E. Raz, and D. H. Broide
Accumulation of Peribronchial Mast Cells in a Mouse Model of Ovalbumin Allergen Induced Chronic Airway Inflammation: Modulation by Immunostimulatory DNA Sequences
J. Immunol., November 1, 2003; 171(9): 4860 - 4867.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. Kraft, R. J. Martin, S. C. Lazarus, J. V. Fahy, H. A. Boushey, R. F. Lemanske Jr., and S. J. Szefler
Airway Tissue Mast Cells in Persistent Asthma: Predictor of Treatment Failure When Patients Discontinue Inhaled Corticosteroids
Chest, July 1, 2003; 124(1): 42 - 50.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C. L. Hofstra, P. J. Desai, R. L. Thurmond, and W.-P. Fung-Leung
Histamine H4 Receptor Mediates Chemotaxis and Calcium Mobilization of Mast Cells
J. Pharmacol. Exp. Ther., June 1, 2003; 305(3): 1212 - 1221.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
R I Ketchell, M D'Amato, M W Jensen, and B J O'Connor
Contrasting effects of allergen challenge on airway responsiveness to cysteinyl leukotriene D4 and methacholine in mild asthma
Thorax, July 1, 2002; 57(7): 575 - 580.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
Leader of the Working Group: A. Efthimiadis, A. Sp, Members of the Working Group:, Q. Hamid, M.M. Kelly, M. Linden, R. Louis, M.M.M. Pizzichini, E. Pizzichini, C. Ronchi, F. Van Overveld, et al.
Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation
Eur. Respir. J., July 1, 2002; 20(37_suppl): 19S - 23s.
[Full Text] [PDF]

Home page
 Eur Respir JHome page
N.G. Carroll, S. Mutavdzic, and A.L. James
Distribution and degranulation of airway mast cells in normal and asthmatic subjects
Eur. Respir. J., May 1, 2002; 19(5): 879 - 885.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Asthma, Airway Biology, and Allergic Rhinitis in AJRCCM 2000
Am. J. Respir. Crit. Care Med., November 1, 2001; 164(9): 1559 - 1580.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
G. M. GAUVREAU, K. N. PARAMESWARAN, R. M. WATSON, and P. M. O'BYRNE
Inhaled Leukotriene E4, But Not Leukotriene D4, Increased Airway Inflammatory Cells in Subjects with Atopic Asthma
Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): 1495 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. Van Den BERGE, H. A. M. KERSTJENS, R. J. MEIJER, D. M. DE REUS, G. H. KOETER, H. F. KAUFFMAN, and D. S. POSTMA
Corticosteroid-induced Improvement in the PC20 of Adenosine Monophosphate Is More Closely Associated with Reduction in Airway Inflammation than Improvement in the PC20 of Methacholine
Am. J. Respir. Crit. Care Med., October 1, 2001; 164(7): 1127 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
G. M. GAUVREAU, G. M. RONNEN, R. M. WATSON, and P. M. O'BYRNE
Exercise-induced Bronchoconstriction Does Not Cause Eosinophilic Airway Inflammation or Airway Hyperresponsiveness in Subjects with Asthma
Am. J. Respir. Crit. Care Med., October 1, 2000; 162(4): 1302 - 1307.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2000 American Thoracic Society 		  Red Transition